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Citations for Mabtech :
101 - 150 of 177 citations for Flap endonuclease 1 FEN 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: IFN-γ ELISpot assays were performed using the Human IFN-γ ELISpot PLUS ALP kit (Mabtech) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Immunology 2023Quote: ... Washed plates were incubated with alkaline-phosphatase-conjugated mAb for IFN-γ (Mabtech #7-B6-1) and with biotinylated mAb for IL-2 (Mabtech #MT8G10) ...
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bioRxiv - Immunology 2019Quote: IL-8 was quantified in plasma by Human IL-8 (CXCL8) ELISA development kit (3560-1H, Mabtech) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: T cell responses were assessed by ELISPOT assay using a Human IFNγ ELISPOTPro kit (Mabtech, NS, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay was performed on treated PBMCs using ELISpot Pro: Human IFN-γ (ALP) kit (Mabtech, Sweden), following manufacture’s protocol ...
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bioRxiv - Immunology 2021Quote: ... supplemented with the TLR7 and TLR8 agonist imidazoquinoline resiquimod (R848, 1 μg/ml; Mabtech AB, Nacka, Sweden), and recombinant human IL-2 (10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution; Mabtech) after overnight incubation ...
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bioRxiv - Immunology 2020Quote: ... supplemented with the TLR7 and TLR8 agonist imidazoquinoline resiquimod (R848, 1 µg/ml; Mabtech AB, Nacka, Sweden), and recombinant human IL-2 (10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...
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bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 1 μg/ml of the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Mabtech) diluted in PBS-0.5% FCS for 2 h ...
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bioRxiv - Immunology 2020Quote: ... and immediately washed 6x with 200μL ddH2O and then coated with 100μL/well of PBS + 15μg/mL primary anti-human granzyme B antibody (clone GB10, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were detected by Human IFN-γ ELISpot Basic Kit (ALP) (Mabtech AB, 3420-2A). The plates were developed using the AP Conjugate Substrate Kit (Biorad ...
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bioRxiv - Immunology 2020Quote: ... On Day 7 of expansion the CD8 population was assessed by Human IFN-γ 96 well ELISPOT (Mabtech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Immunology 2020Quote: ... a commercial COVID peptide pool (SARS-CoV-2 S N M O defined peptide pool [3622-1] – Mabtech), and CEF were included as assay controls at a concentration of 1µg/ml ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-mouse IFN-γ R4-6A2 mAb (1:1000 dilution; Mabtech) diluted in PBS with 1% FBS for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... The CD40L-biotin was detected by adding a 1:1,000 dilution of Streptavidin-HRP (Mabtech, 3310-9-1000) and the signal was developed by addition of TMB substrate (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was detected by addition of alkaline phosphatase-conjugated anti-human IgG (clone MT78; Mabtech, Cincinnati, OH), followed by p-nitrophenyl phosphate substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... The ELISpot assay was performed on treated PBMCs using the ELISpot Pro: Human IFN-g (ALP) kit (Mabtech, Sweden), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Detection of Perforin and CHI3L1 in iNKT cell culture supernatants was performed using the Human Perforin Elisa Flex (Mabtech) and the Human CHI3L1 DuoSet ELISA (R&D systems ...
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bioRxiv - Bioengineering 2021Quote: ... and wells incubated with 100 µL of 1 µg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2020Quote: ... and wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2023Quote: ... The plates were then washed 5 times with PBS and a 1:1000 dilution of ALP-conjugated streptavidin (Mabtech) was added ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2021Quote: ... and IgA production by each sorted B-cell subset were evaluated by using the Human IgG/IgM and IgG/IgA FluoroSpot kits (MABTECH), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IL-6 levels in serum from patients and healthy controls were measured in freshly thawed serum using human IL-6 ELISA development kit (Mabtech), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... all at 10 µg/ml except for the CEF pool (CEF extended peptide pool for human CD8 T cells, Mabtech) used at 5 µg/ml and cultured overnight at 37°C ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... The IFNγ+ MAIT cell spots were then developed with an indirect immunostain approach sequentially using a biotinylated anti-human IFNγ antibody (Mabtech), ExtraAvidin conjugated by alkaline phosphatase (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and the wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 6x times with PBST and plated with streptavidin-HRP conjugate (1:1000 dilution; catalogue #3310-9-1000, Mabtech) for 1 hour at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... This method is similar to before(Depla et al., 2008) and were performed using a Human IFN-gamma ELISpot PLUS kit (Mabtech, Sweden) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... The number of B cells secreting SARS-CoV-2 RBD-specific IgG and total IgG were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS kit (Mabtech AB)6.
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bioRxiv - Immunology 2020Quote: IFN-γ and IL-2 secreting T cells were detected using Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kits according to the manufacture’s protocol (Mabtech AB). The plates pre-coated with capturing monoclonal anti-IFN-γ and anti-IL-2 were incubated overnight in RPMI-1640 medium containing 10% FCS supplemented with a mixture containing the SARS-CoV-2 peptide pool (scanning or defined pools) ...
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled freshly recombinantly produced proteins and the mAb 107 of the Human IgE ELISA development kit HRP (Mabtech, Nacka Strand, Sweden), which was used for standardization ...
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bioRxiv - Bioengineering 2023Quote: ... were collected for ELISpot assays using ELISpot Plus: Monkey IFN-γ (HRP) and ELISpot Plus: Human IL-4 (HRP) kits (Mabtech). 2 × 105 PBMCs seeded onto 96 well plates in the kits were incubated with 1 μg of PepMixTM SARS-CoV-2 (Spike Glycoprotein ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2019Quote: ... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...