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Citations for Mabtech :
101 - 150 of 198 citations for 7 Boc 3 chloro 5 6 7 8 tetrahydroimidazo 1 2 a pyrazine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2020Quote: ... An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech, Nacka, Sweden). The cells were assayed at 2 x 105 cells per well ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgA biotin (Mabtech, Cat no. 3865-6-250) at (1:500 ...
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bioRxiv - Immunology 2021Quote: ... The remaining time-points were evaluated using Monkey IFNγ/IL-4 FluoroSpot FLEX kit and Monkey IL-2 FluoroSpot FLEX kit (Mabtech, X-21A16B and X-22B) following manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 6 times and the pNPP solution (Mabtech, Sweden) was added at 50 μl per well ...
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bioRxiv - Immunology 2023Quote: ... The plates were then washed 5 times with PBS and a 1:1000 dilution of ALP-conjugated streptavidin (Mabtech) was added ...
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bioRxiv - Immunology 2021Quote: ... followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250, RRID:AB_907273) and visualization by Streptavidin horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2020Quote: ... Plates were substrated for 8-12 minutes using 0.5µM filtered 3’3’5’5’-Tetramethylbenzidine (TMB substrate) (Mabtech, 3652-F10) and the plates were evaluated with an AID automated microplate ELISpot reader and associated software (AID ELISpot Version 7.0).
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bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
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bioRxiv - Immunology 2023Quote: ... mouse IFN-γ/IL-5 (X-41A43B; Mabtech) and IFN-γ/IL-2/TNF-α (X-41A42B45W ...
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bioRxiv - Immunology 2021Quote: ... IFN-α was quantified from culture media using a pan-subtype ELISA (MABTECH 3425-1H-6).
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bioRxiv - Immunology 2024Quote: ... then incubated at room temperature with biotinylated secondary goat anti-mouse IgG (catalogue #3825-6-250, Mabtech) at a 2 µg/mL concentration in the dark for 2 hours ...
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bioRxiv - Microbiology 2020Quote: Murine IFN-γ and IL-5 ELISPOT assays were performed following manufacturer’s procedures for mouse IFN-γ and IL-5 ELISPOT kit (Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2020Quote: ... In some experiments an IL-5 development ELISA was used (Mabtech). Measured concentrations were then adjusted to pg cytokine per million PBMC at the start of the assay ...
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bioRxiv - Immunology 2020Quote: IL-6 levels in serum from patients and healthy controls were measured in freshly thawed serum using human IL-6 ELISA development kit (Mabtech), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... we washed off the cells and developed the plates using biotinylated R4-6A2 anti-IFN-γ (Mabtech #3321-6-250) and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250 ...
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bioRxiv - Immunology 2020Quote: ... The plate was washed and IFN-I assayed using a mixture of biotinylated detection antibodies against IFN-α (Mabtech, MT2/4/6). Avidin-conjugated alkaline phosphatase (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Secreted levels of IL-1ß and IL-6 were quantified using sandwich ELISA according to the manufacturer’s instruction (MabTech AB, Nacka, Sweden). Absorbance was measured at a wavelength of 405 nm using a microplate reader (Molecular Devices Corp ...
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bioRxiv - Genomics 2020Quote: ... Cells were stimulated with soluble anti-CD3 (5 μg/mL; MabTech, Cincinnati, OH) and anti-CD28 antibodies (10ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... and Granzyme B (Biozol, #3485-1H-20 for CD276- and CD19-CAR T cells; MabTech, #3486-1H-6 for F8-FR4-CAR T cells) following the manufactures’ instructions ...
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bioRxiv - Immunology 2023Quote: TNFα ELISA was performed with the supernatant from T cell and LCL co-culture as described above using the human TNFα ELISABASIC kit (Mabtech, Cat. 3512-1H-6), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl of anti-mouse IFN-γ mAb AN18 (5 µg/ ml; Mabtech) overnight at 4°C before being washed and blocked with cRMPI 10% FBS for 1 hr at 37C ...
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bioRxiv - Microbiology 2023Quote: ... that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb) AN18 (Mabtech). The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech ...
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bioRxiv - Microbiology 2022Quote: ... or IL-5 were determined using a mouse enzyme-linked immunospot (ELISpot) assay kit (Mabtech, Cincinnati, OH) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... plates were coating with 50 μL of anti-porcine IFN-γ mAb pIFNγ-I (5 µg/ ml; Mabtech) and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution ...
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bioRxiv - Immunology 2020Quote: ... and 5 μg/mL anti-perforin capture antibody mix (capture αPrf; Mabtech, combination of clones Pf-80/164). The resulting surfaces were micropatterned as described before with 10 μg/mL αLFA-1 + 10 μg/mL αCD16 + 5 μg/mL capture αPrf + 10 μg/mL BSA-AF555 ...
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bioRxiv - Immunology 2020Quote: ... The detection antibody solution composed of PBS + 5 μg/mL anti-perforin conjugated with biotin (clone Pf-344, Mabtech) was added to the slide chambers ...
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bioRxiv - Immunology 2019Quote: ... were co-cultured with GLC peptide-specific T cells (5×104 cells/ per well) for 20 hours as per manufacturer’s protocol (Mabtech, Sweden).
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bioRxiv - Immunology 2019Quote: ... NS1-specific IFNγ and IL-5 production by ZIKV-specific splenic T cells were enumerated by Enzyme Linked ImmunoSpot (ELISPOT) assay following manufacturer’s instructions (Mouse IFN-γ/IL-5 ELISpot Basic kits; Mabtech). Mouse splenocytes were left unstimulated or stimulated with an NS1 peptide pool (PepMix ZIKV NS1 Ultra ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS, Mabtech). Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech) ...
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bioRxiv - Immunology 2021Quote: ... Reactivity was assessed by specific IFN-γ release by ELISpot assay by coating ELISpot plates (MAHAS4510) with IFN-γ capture antibody (MABTECH Cat# 3420-3-250, RRID:AB_907283) followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250 ...
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bioRxiv - Immunology 2021Quote: Murine IFN-γ and IL-5 ELISpot assays were performed following the manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2021Quote: ... (mAbs 1-D1K; Mabtech, Stockholm, Sweden) at a concentration of 10ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-HRP (Mabtech – 1/1000 dilution) was added for 30 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 1 hour with streptavidin-HRP (diluted 1:100 with D-PBS, Mabtech, 3310-9-1000), and then washed again ...
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bioRxiv - Microbiology 2022Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2023Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 at a concentration of 1×105 cells/mL media on plates previously coated with anti-human IFNγ (Mab 1-D1K; Mabtech, Stockholm, Sweden). Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1 ...
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bioRxiv - Immunology 2020Quote: ... Controls included 1×105 effector CD8+ T cells co-cultured with anti-human CD3 monoclonal antibody (0.5 µg/mL, Mabtech, 3605-1-1000), or with media alone ...
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-alkaline phosphatase (Mabtech, 1:1,000 dilution). Spots were visualised using BCIP/NBT plus substrate and counted via ELISPOT plate reader.
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-alkaline phosphatase (Mabtech; 1:1,000 dilution). Spots were visualised using BCIP/NBT plus substrate (Mabtech ...
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bioRxiv - Immunology 2021Quote: ... M ORF3a and ORF7a proteins (#3622-1, Mabtech AB)57 ...
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bioRxiv - Bioengineering 2021Quote: ... with 1:1000 diluted streptavidine-ALP preparations from Mabtech. After washing ...
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bioRxiv - Cancer Biology 2021Quote: ... with 1:1000 diluted streptavidine-ALP preparations from Mabtech. After washing ...
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bioRxiv - Immunology 2020Quote: ... M ORF3a and ORF7a proteins (#3622-1, Mabtech AB) 19 ...
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bioRxiv - Immunology 2020Quote: ... with 1:1000 diluted streptavidine-ALP preparations from Mabtech. After washing ...