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Citations for Mabtech :
101 - 150 of 211 citations for 7 8 9 10 TETRAHYDRO 6H AZEPINO 2 1 B QUINAZOLIN 12 YLIDENEAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse IL-17A ELISpotPLUS kit ALP (MABTECH, Cat# 3521-4APW-2) was performed following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... coated with IFN-γ capture antibody (10 mg/mL; Mabtech) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the Monkey IFN-γ ELISpotPLUS kit (MABTECH 3421M-APT-10) was used as directed by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and Monkey IL17A ELISpotPLUS Kit (Mabtech, Cat #3520M-4APW-10) were used following manufacturer’s instructions with 2×105 freshly isolated PBMC added to each well ...
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bioRxiv - Immunology 2022Quote: ... The 96-well ELISpot plates (MabTech, Cat# 3654-TP-10) were pre-wetted with 35% ethanol and washed five times with sterile water plus 1 time with PBS ...
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bioRxiv - Immunology 2021Quote: ... Each peptide pool (2 µg/ml) and αCD28 (0.1 µg/ml, Mabtech) were added to the cells and plates were incubated for 48h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... were coated with monoclonal antibodies to bovine IFNγ and IL-2 (MABTECH). Duplicate wells were seeded with 1×105 cells per well of cryopreserved bovine PBMCs (rested overnight before use ...
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bioRxiv - Immunology 2021Quote: ... or monoclonal anti-human CD3 (Mabtech, Sweden, Cat. 3420-2AST-10) at a 1:1000 dilution for positive control wells ...
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bioRxiv - Immunology 2021Quote: Monkey IFN-γ ELISpotPro plates (Mabtech, Sweden, Cat#3421M-2APW-10) were prepared according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI 1640 media (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... we used antigen-specific IFN-γ ELISpot kits (MABTECH, 3321-4HPT-10) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... wells were washed and incubated with Streptavidin-ALP (Mabtech, Cat. 3310-10) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Monkey IFN-γ ELISpotPro (alkaline phosphatase) plates (Mabtech, Sweeden, Cat#3421M-2APW-10) were blocked for a minimum of 2 hours with RPMI 1640 (Corning) ...
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bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ and IL-2-secreting cells were detected using ELISpot kits (DAKEWE and MABTECH) according to the manufacture protocol and spots were counted using an ELISpot Analyzer AT-SPOT(SINSAGE).
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bioRxiv - Immunology 2024Quote: ... Frequencies of IFN-γ or IL-2-secreting T cells were assessed using commercial kits (Mabtech) according to the manuals ...
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bioRxiv - Immunology 2022Quote: ... IFNγ ELISPOT was performed using the Mouse IFNγ ELISPOTPLUS kit (MABTECH product # 3321-4HPW-10). ELISPOT plates were analyzed using ImmunoSpot CTL plate reader (CTL ImmunoSpot S6 Entry Analyzer ...
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bioRxiv - Immunology 2024Quote: ... then followed by addition of 100 µL/well of ELISpot substrate (catalogue #3651-10; Mabtech). Plates were incubated in the dark for up to 25 minutes while the spot formation was closely monitored ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Immunology 2022Quote: ... we developed the spots by adding 70 µL/well of BCIP/NBT-plus substrate (Mabtech #3650-10) to the wells ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS, Mabtech). Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech) ...
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bioRxiv - Microbiology 2022Quote: IL-4 and IL-2 ELISPOT assays were performed with mouse IL-4 ELISPOTPLUS kits and mouse IL-2 ELISPOTPLUS kits according to the manufacturer’s instructions (Mabtech). IFN-γ ELISPOT assays were performed with mouse IFN-γ Precoated ELISPOT kits according to the manufacturer’s instructions (Dakewe Biotech) ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 S antigen-specific T lymphocyte response in mice was assessed by IFN-γ ELISpotPLUS kits (Mabtech) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Pathology 2022Quote: ... The cells were incubated for 20 hours at 37°C in 5% CO2 incubator in pre-coated Ferret IFNγ-ELISpot plates (3112-4APW-2, Mabtech). The assay was performed following the manufacturers protocol ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... the 2CZ T cell hybridoma was used and the IL-2 secreted upon its activation was quantified using an ELISPOT assay (Mabtech) as previously described 7.
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bioRxiv - Immunology 2021Quote: ... (mAbs 1-D1K; Mabtech, Stockholm, Sweden) at a concentration of 10ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-HRP (Mabtech – 1/1000 dilution) was added for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay plates were prepared and processed as per the manufacturer’s instructions (3321-4HPT-10, Mabtech Inc., Cincinnati, OH). ELISpots were enumerated by machine (CTL S6 Entry M2 ...
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Microbiology 2022Quote: ... all at 10 µg/ml except for the CEF pool (CEF extended peptide pool for human CD8 T cells, Mabtech) used at 5 µg/ml and cultured overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... plates were developed using reagents and following procedures and incubation periods described in the pig IFNγ ELISpot Plus kit (3130-4HPW-10, Mabtech). Spots were counted under a dissection microscope (Leica GZ6) ...
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bioRxiv - Immunology 2023Quote: ... Commercially available ELISPOT plates for evaluation of IL-4 (MuIL4, Immunospot, Cellular Technology Limited) and IFNg (MuIFNg, Immunospot, Cellular Technology Limited; cat# 3110-4APW-10, rabbit, MabTech) were used ...
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bioRxiv - Immunology 2023Quote: ... Wells were then washed again and spots were developed with filtered substrate solution BCIP/ NBT-plus (Mabtech, Cat. 3650-10). When distinct spots were visible ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Microbiology 2022Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2023Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... pulsed with WT1328-348 (10 µg/ml) followed by measurement of specific cytokine production using the human IFN-γ ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.