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Citations for Mabtech :
51 - 100 of 126 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Plates were then washed 6x with PBS and 0.5μg/mL of Streptavidin-ALP (Mabtech) was added to IFN-γ plates at 100μL/well ...
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bioRxiv - Immunology 2020Quote: ... ELISpot assays were performed using the Macaca fascicularis IFN-γ ELISpot PLUS plates (MABTECH). 96-well ELISpot plates precoated with capture antibody were blocked with RPMI1640 medium overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The number of spot-forming units was enumerated using an ELISPOT plate reader (Mabtech).
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bioRxiv - Immunology 2024Quote: ... and plates were incubated with biotinylated IFNγ monoclonal antibodies (MAb) (7-B6-1; Mabtech) for 2 h at 37°C and developed as described previously39 ...
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bioRxiv - Cancer Biology 2024Quote: Cultured T cells were transferred to an enzyme-linked immunospot (ELISpot) plate (Mabtech, Sweden) and incubated for 20 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Afterwards plate was washed with PBS and Streptavidin-550 (Mabtech, Cat no. 3310-11-1000) diluted in PBS-0.1% BSA 1:200 was added ...
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bioRxiv - Immunology 2020Quote: ... plates were washed and incubated with anti-IgG-biotin and/or anti-IgA-biotin (Mabtech) for 2 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
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bioRxiv - Immunology 2020Quote: ... and the plates were incubated with the detection antibody 7-B6-1-biotin (Mabtech, Sweden) diluted 1:1000 in PBS + 0.5% FCS and incubated for two hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, MabTech), followed by incubation with Streptavidin-AP (MabTech ...
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bioRxiv - Immunology 2020Quote: ... aliquots of PBMC (105/well) were seeded in duplicate wells of 96-well plates (MABTECH) pre-coated with the primary IFN-γ antibody and stimulated with mitogens PHA ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2023Quote: ... Developed spots on the ELISpot plate were then enumerated using an ELISpot reader (Mabtech, Sweden). The reactivity was determined by measuring the fold increase in the number of spots of PBMCs treated with mutant peptides relative to those treated with wild type peptides ...
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bioRxiv - Cancer Biology 2024Quote: ... Developed spots on the ELISpot plate were then counted using an ELISpot reader (Mabtech, Sweden). Reactivity was determined by measuring the fold increase in the number of spots in PBMCs treated with mutant peptides compared to those treated with wild-type peptides ...
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bioRxiv - Microbiology 2022Quote: ... The air-dried plates were read using the automated ELISPOT reader (Mabtech IRIS FluoroSpot/ELISpot reader) for calculating spot-forming cells.
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bioRxiv - Immunology 2023Quote: ... Washed plates were incubated with alkaline-phosphatase-conjugated mAb for IFN-γ (Mabtech #7-B6-1) and with biotinylated mAb for IL-2 (Mabtech #MT8G10) ...
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bioRxiv - Immunology 2022Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech; 1:2000), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... Plates were substrated for 8-12 minutes using 0.5µM filtered 3’3’5’5’-Tetramethylbenzidine (TMB substrate) (Mabtech, 3652-F10) and the plates were evaluated with an AID automated microplate ELISpot reader and associated software (AID ELISpot Version 7.0).
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bioRxiv - Immunology 2020Quote: ... 96 well plates were coated with a mixture of antibodies against IFN-α (Mabtech, MT1/3/5) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2024Quote: ... 1×104 CD3+ T cells/well were plated in pre-coated IFNγ/IL2/TNF Fluorospot plates (Mabtech) and cultured overnight in medium alone (negative control ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were plated in each well of the pre-coated Immobilon-FL PVDF 96 well plates (Mabtech), stimulated with the respective antigen at the respective concentration of 5 μg/ml and incubated at 37°C in a humidified CO2 incubator for 20-24 hrs ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS and then incubated with anti-monkey or anti-mouse IFNγ mAb biotin (Mabtech) for two hours ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed a further six times and developed for 20 min with BCIP/NBT Substrate (Mabtech). All assays were performed in duplicate ...
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bioRxiv - Immunology 2020Quote: ... plates were coating with 50 μL of anti-porcine IFN-γ mAb pIFNγ-I (5 µg/ ml; Mabtech) and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution ...
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bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Then plates were washed with PBST and 50 µl of 1 µg/ml streptavidin (Mabtech, 3310-9-1000) was added to each well of ELISpot plates ...
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bioRxiv - Microbiology 2021Quote: ... Splenocytes were counted and 100,000 cells/well added to the plates of the Mouse IFN-g ELISpotPLUS (HRP) kit (MABTECH). Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... Miltenyi Biotech) for 24 h in ELISpot plates coated with anti-mouse-IFN antibody (Mabtech AB, Nacka Strand, Sweden). The procedures were carried out according to the manufacturer’s instructions (Mabtech ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay plates were prepared and processed as per the manufacturer’s instructions (3321-4HPT-10, Mabtech Inc., Cincinnati, OH). ELISpots were enumerated by machine (CTL S6 Entry M2 ...
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bioRxiv - Immunology 2024Quote: ... plates were washed with PBS and biotinylated anti-human IFNγ antibody (2.5 μg mL-1, 7-B6-1, MabTech) was added for 2 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: Plates were coated overnight at 4°C with an antibody mixture containing mouse anti-human IFNγ (Mabtech, 1-D1K). The coated plates were then prepped with stimuli as follows ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Pathology 2022Quote: ... The cells were incubated for 20 hours at 37°C in 5% CO2 incubator in pre-coated Ferret IFNγ-ELISpot plates (3112-4APW-2, Mabtech). The assay was performed following the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody and Streptavidin-HRP were added successively after cell lysis.Then AEC Peroxidase Substrate were added and spots counted using an ELISpot plate reader (Mabtech IRIS™ Fluorospot Reader, Mabtech). Spot numbers were evaluated using Mabtech Apex™ software v.1.1.52.121.
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS/0.05% Tween 20 and incubated with streptavidin – alkaline phosphatase conjugate (Strep-ALP, Mabtech AB, Sweden). After a final wash ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed six times and developed using VECTASTAIN® Elite ABC-HRP according to the manufacturer’s instructions (Mabtech). All assays were performed in duplicate ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS/0.05% Tween 20 and incubated with streptavidin – alkaline phosphatase conjugate (Strep-ALP, Mabtech AB, Sweden). After a final wash ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2022Quote: ... we washed off the cells and developed the plates using biotinylated R4-6A2 anti-IFN-γ (Mabtech #3321-6-250) and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250 ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...