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Citations for PerkinElmer :
51 - 100 of 100 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
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bioRxiv - Biophysics 2019Quote: ... The bead slurry was incubated at RT for 30 minutes before reading on an EnVision plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
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bioRxiv - Genomics 2022Quote: ... The slides were blocked in TNB buffer at RT for 30 minutes and incubated with antifluorescein-HRP Conjugate (PerkinElmer) in TNB (diluted 1:1000 ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...
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bioRxiv - Molecular Biology 2022Quote: ... The reverse primer contained an Illumina 3’ adapter sequence and an index sequence to uniquely identify each library (NextFlex DNA barcodes, PerkinElmer). Libraries were purified and quantified using the dsDNA Fluorescence Quantification Assays (DeNovix ...
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bioRxiv - Biophysics 2021Quote: ... Plates were incubated at RT for 40 min and read according to the protocol using an EnVision multilabel reader (PerkinElmer) with the emission window ratio of 665 nm over 620 nm ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated in the dark for 2 h at RT before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of FITC 485 / FITC 535 ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and incubated with TSA Plus Fluorescein (PerkinElmer) according to the manual at RT for 3-15 minutes (3-5 min in H ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and the amplification steps were repeated using anti-DNP-HRP Conjugate (PerkinElmer) and TSA Plus Cyanine 3 (PerkinElmer) ...
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bioRxiv - Biophysics 2023Quote: ... The absorption and emission spectra of the purified protein were recorded at RT (Lambda365, Perkin Elmer and FLS980, Edinburgh Instruments)
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... For the analysis of Dscam and elav alternative splicing primers were labeled with 32P gamma-ATP (6000 Ci/ mmol, 25 mM, Perkin Elmer) with PNK to saturation and diluted as appropriate 69 ...
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bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Microbiology 2021Quote: ... The plate was then incubated for 1h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (Perkin Elmer). Negative control with binding buffer was used to control the assay quality ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were thawed and fixed for two min with 0.1% paraformaldehyde in PBS at RT and then incubated in 50 pM I125-OVTA (2200 Ci/mmol; PerkinElmer; Boston, MA), a selective ...
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bioRxiv - Molecular Biology 2019Quote: ... Two probes were amplified from a plasmid template targeting the pBac arms (see Table S1 for primers) and labelled with CTP α-32P 3000Ci/mmol (Perkin Elmer) using Klenow Enzyme (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... The reverse primer R3 (5′-GCGCCGGTAGTTGCCCTTCTC-3′) was labeled at the 5′ end using [γ-32P]-ATP (PerkinElmer Inc., Waltham, MA, USA) and T4 polynucleotide kinase (Takara) ...
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bioRxiv - Biochemistry 2021Quote: ... the R2R and primer extension (PE) DNA primers (100 pmol) were labeled with [γ-32P]ATP (125 pmol; 6,000 Ci/mmol; 150 μCi/ μl; PerkinElmer Life Sciences) by incubating the DNA with T4 polynucleotide kinase (10 units ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 μl of Universal PCR Master Mix (PerkinElmer Applied Biosystems) and 7.7 μl of nuclease-free water ...
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bioRxiv - Bioengineering 2022Quote: ... The reactions were terminated with 1M H3PO4 after 20 minutes incubation at RT and optical density (OD) was measured by a spectrophotometer at 450nm (PerkinElmer EnVision 2105).
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bioRxiv - Bioengineering 2022Quote: ... The reactions were terminated with 1M H3PO4 after 20minutes incubation at RT and optical density (OD) was measured by a spectrophotometer at 450nm (PerkinElmer EnVision 2105).
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bioRxiv - Microbiology 2023Quote: ... Cycle sequencing was carried out using both forward and reverse primer of each fragment in a GeneAmp9600 thermal cycler (Perkin Elmer, Waltham, USA) employing 30 cycles at 96°C for 10 s ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR products were validated using a LabChip GX II (Perkin Elmer) and sequenced using PlateSeq PCR Kits (Eurofins ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were used as template to incorporate radioactive 32P-dCTP (Perkin Elmer) using the High Prime kit (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were then transferred to GeneScreen Plus Hybridization Transfer Membrane (Perkin Elmer) and detected by hybridization using a non-radioactive method as described in Tomé et al.30.
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were sequenced with the BigDye terminator kit (Perkin Elmer Applied Biosystems) using ABI3100 capillary sequencer (Perkin Elmer Applied Biosystems) ...
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bioRxiv - Biochemistry 2020Quote: ... Quantitative PCR (qPCR) was performed on an ABI 7900HT sequence detection system (Perkin Elmer, Applied Biosystems) using TaqMan™ Gene Expression Assays (FAM-labelled ...
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were cleaned with the Biomek FX robot using the chemagic SEQ Pure20 Kit (PerkinElmer, CMG-458). Clean PCR amplicons from the same DNA sample were pooled to generate 137 libraries that were sequenced by Illumina MiSeq v3 ...
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bioRxiv - Genetics 2021Quote: ... DNA fragments were detected using a mix of five probes labelled by PCR amplification with 32P dCTP (Perkin Elmer) described in Fig S1 ...
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bioRxiv - Plant Biology 2023Quote: ... The size and abundance of the resultant PCR products were then analyzed and quantified by LabChip GX bioanalyzer (PerkinElmer). The expression analyses represent two to three biological replicates ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... Probes were prepared by random priming of corresponding PCR products (5’ and GFP templates) in the presence of [αP32]CTP (PerkinElmer BLU513H100UC). Unincorporated nucleotides were removed using a Sephadex G-50 column (GE Healthcare 28-9034-08) ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 120 ng PCR product was mixed with 7.5 mM ribonucleotide solution (ATP, GTP, UTP, CTP) and 133 nM α-32P-ATP (Perkin Elmer) as a tracer ...
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bioRxiv - Immunology 2022Quote: ... The PCR products were analyzed by heteroduplex mobility assay using microfluidic capillary electrophoresis system caliper LabChip GX (PerkinElmer, Villebon-sur-Yvette, France). The homozygous Il34-/- specimen were identified from Il34+/+ littermate as previously described (13).
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bioRxiv - Molecular Biology 2023Quote: ... were prepared in in vitro transcription reaction on linear PCR-generated template containing RNAImod (4) with 500 μM ATP or NAD and 25 μM [α 32P] CTP (Perkin Elmer) using hexa-histidine tagged E ...
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bioRxiv - Molecular Biology 2019Quote: ... the templates of dsRNA were amplified from the above pBluescript KS (+) vectors inserted diap1 sequences in each insect by PCR using AmpliTaq Gold (Perkin Elmer, Boston, USA). The PCR primers are described in Table S8 (T7-KS/T7-SK) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were added to the PCR product and sequencing was conducted using the automated DNA Sequencer (ABI 3130xl Genetic Analyzer; Perkin Elmer/Applied Biosystems). Using the software Genemapper ver ...
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bioRxiv - Molecular Biology 2020Quote: ... Double-stranded DNA probes were synthesized by random-prime labeling of PCR fragments with [α-32P]dATP and [α-32P]dTTP (PerkinElmer, Waltham, MA). Probe locations are as follows with numbering relative to the start codon of the indicated protein-coding gene ...