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Citations for PerkinElmer :
151 - 200 of 844 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Physiology 2021Quote: ... Serum insulin was analyzed using time-resolved immunofluorometric assay (AutoDELFIA, PerkinElmer, Finland). Raw counts of the RNA-seq gene reads were normalized by variance stabilizing transformation.
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bioRxiv - Cancer Biology 2023Quote: ... BLI signal was tracked over time using Living Image software (Perkin Elmer). BLI signal at endpoint was log-transformed and means were compared between the vehicle and control groups with a two-tailed t test ...
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bioRxiv - Immunology 2023Quote: ... before Time-Resolved Fluorescence (TRF) was detected by Envision plate-reader (PerkinElmer). TRF of target cell alone medium was set as spontaneous release ...
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bioRxiv - Cell Biology 2019Quote: ... detection of proteins from the membranes was performed with Western Lightning ECL Pro solution (Perkin Elmer, #NEL121001EA).
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bioRxiv - Molecular Biology 2020Quote: ... pH 6.8).(3) Detection reactions were conducted in 96-well black half-area microplates (PerkinElmer, Waltham, MA) and sealed with MicroAmp optical adhesive film (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: AlphaScreen cMyc detection and Proxiplate-384 Plus 384-wells plates were purchased from Perkin Elmer (MA, USA). Lewy body proteins bearing a N-terminal GFP tag and α-SYN labeled with N-terminal mCherry-Myc were co-expressed in the cell-free system by adding mixed DNA vectors in 10 μL of the Leishmania tarentolae-based cell-free system (to a final DNA concentration of 30 nM for the GFP-vector and 60 nM for the Cherry-vector) ...
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bioRxiv - Developmental Biology 2019Quote: ... Both instruments were modified to enable detection using silicon Avalanche Photo Detectors (SPCM-AQR-1X; PerkinElmer, USA) for imaging and FCS ...
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bioRxiv - Developmental Biology 2023Quote: ... we used the Tyramide signal amplification (TSA) detection system (PerkinElmer, MA, USA, or Biotium, Inc. CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The detection of cAMP-dependent expression was performed using an Envision plate reader (PerkinElmer, Waltham, MA, USA), based on luciferase-based luminescence ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at RT with following blocking with TSA blocking reagent (Perkin Elmer, Waltham, MA, USA, FP1020) as 0.5% solution in PBS pH 7.4 for another 1h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were incubated at RT for 30 min and measured with EnVision multi-plate reader (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... Detection reactions were incubated for 3 hours at 37°C on a VICTOR Nivo fluorescent plate reader (PerkinElmer) with fluorescence measurements taken every 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Intrahepatic tumor growth was assessed by live luciferase detection using IVIS Lumina II CCD camera system (Perkin Elmer). Data were acquired and analysed using the Living Image Software version 4.3 (Perkin Elmer) ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were treated with the Western Lightning Plus ECL detection reagent (PerkinElmer Life Sciences, catalog no. NEL103001EA) or Clarity Western ECL substrate (Bio-Rad) ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... After the detection of the radioactive signals with a PhosphoImager (GE Healthcare Perkin Elmer Cyclone Typhoon FLA 7000), the membrane was incubated for 1 h at RT in 6 M HCl ...
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bioRxiv - Microbiology 2023Quote: ... ATP production was measured as an indicator of viability using ATPlite™ Luminescence ATP Detection Assay System (PerkinElmer,). Luminescence was read using BioTek Synergy Hybrid Multi-Mode reader (BioTek,) ...
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bioRxiv - Plant Biology 2023Quote: ... All peaks were examined manually for background detection and interference using Syngistix for ICP version 3.0 (Perkin Elmer).
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bioRxiv - Immunology 2019Quote: ... and scanned at RT using 20X objective on the Vectra® 3 automated quantitative pathology imaging system (PerkinElmer) (Fig ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 30 min at RT with streptavidin-HRP (Perkin Elmer) dil ...
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bioRxiv - Microbiology 2020Quote: ... for 5 min at RT and measured at 450 nm by the microplate reader Victor Nivo (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: Culture supernatants were assayed for RT activity by surveying for 3H-thymidine incorporation (3H-TTP; Perkin Elmer #NET221A001MC). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Generated AFC signal is detected after 15 min at RT using Ex/Em = 360/460 nM (Envision, PerkinElmer). Final assay concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... and the width/length ratio were calculated over time using Harmony software (PerkinElmer).
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bioRxiv - Cell Biology 2019Quote: ... Time-lapse images were acquired at 1s interval using the Volocity software (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... Time traces were acquired for 30 s using a SPQR14 avalanche photodiode (PerkinElmer), and autocorrelation functions were produced in real-time using a Flex02-02D correlation card (Correlator.com) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed three times with PBS and lysed in MicroScint-20 (PerkinElmer). Scintillation counting was performed on a MicroBeta TriLux (PerkinElmer).
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images were acquired at 1s interval using the Volocity software (PerkinElmer).
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bioRxiv - Cell Biology 2023Quote: ... and time-lapse images were taken and post-processed using Volocity software (PerkinElmer).
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bioRxiv - Immunology 2022Quote: ... PCR products are visualized on Caliper LabChip (PerkinElmer).
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bioRxiv - Microbiology 2021Quote: ... The final Western blotting bands were visualized using an enhanced chemiluminescence protein detection system (PerkinElmer Life Sciences, Boston, MA).
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bioRxiv - Cancer Biology 2022Quote: ... the reaction was stopped using 5 µL 40 µM EDTA in 1X LANCE Detection buffer (Perkin Elmer, CR97-100) for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences, Massachusetts, USA).
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bioRxiv - Pathology 2021Quote: ... Cell viability was measured 72 hours after treatment using an ATPlite 1step detection assay (#6016739, PerkinElmer, Inc., Waltham, MA). To assess synergy between OTX008 and the PI3K inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... and the protein band reacting with the antiserum was detected using a chemiluminescence detection system (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
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bioRxiv - Biophysics 2019Quote: ... The bead slurry was incubated at RT for 30 minutes before reading on an EnVision plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
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bioRxiv - Genomics 2022Quote: ... The slides were blocked in TNB buffer at RT for 30 minutes and incubated with antifluorescein-HRP Conjugate (PerkinElmer) in TNB (diluted 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Agencourt AMPure XP beads and PCR amplified using KAPA hot start High-Fidelity 2X PCR Master Mix and NextFlex index primers (Bioo Scientific, PerkinElmer) for 12 cycle by following thermocycler cycles ...
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bioRxiv - Cell Biology 2019Quote: Time-lapse imaging was acquired using an UltraVIEW Vox spinning-disk microscope (Perkin Elmer) and Volocity software (v.6.3 ...
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bioRxiv - Cell Biology 2021Quote: ... PI+/total cells over time were quantified with using the Harmony Analysis Software (PerkinElmer).
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bioRxiv - Immunology 2021Quote: ... Immunoreactive proteins were detected with either anti-mouse or anti-rabbit horseradish peroxidase-coupled secondary antibody followed by detection with enhanced chemiluminescence (PerkinElmer).
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bioRxiv - Microbiology 2019Quote: ... was achieved by addition of GDP- or UDP-GloTM nucleotide detection reagent following manufacturers’ instructions and analyzed via luminescence using a Victor 2 microplate reader (PerkinElmer).
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bioRxiv - Neuroscience 2022Quote: ... membranes and immunoblots were carried out using primary antibodies (Table S4) followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies (Table S5) and chemoluminiscent detection (Western Lightning, PerkinElmer). All antibodies were diluted in PBS containing 5% semi-skimmed milk and 0.1% Tween-20 ...
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bioRxiv - Developmental Biology 2022Quote: ... A series of incubations was then performed to amplify the signal of the hybridized probe and label target probes for the assigned fluorescence detection channel (target probe was labeled for the assigned fluorescence detection channels Opal 520, Opal 570, Opal 650 and Opal 780, PerkinElmer). Nuclei were stained using a DAPI nuclear stain (RNAscope ref 323108 ...
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bioRxiv - Neuroscience 2020Quote: ... Detection was conducted on white-opaque plates using the Perkin Elmer EnVision 2105 Multimode Plate Reader (Perkin Elmer 2015-0010) using the Ultra-Sensitive Luminescence Detection mode ...
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bioRxiv - Neuroscience 2021Quote: ... Three-plex detection of IL-4Ra on glutaminergic and GABAergic neurons was achieved using Opal 520 (1:1000, Perkin Elmer), Opal 570 (1:1500 ...