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Citations for PerkinElmer :
251 - 300 of 843 citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... The time-resolved fluorescence resonance energy transfer (TR-FRET) was measured on an EnVision plate reader (Perkin Elmer). Activity of the SMG1i against other PI3 kinase family members (PI3Kα ...
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bioRxiv - Genomics 2021Quote: ... Samples were treated with a single probe at a time and in combination with TSA Plus Cyanine5 (PerkinElmer) at a dilution of 1:1500 ...
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bioRxiv - Biochemistry 2021Quote: ... and the luminescence signal was measured in an EnVision Multilabel Plate Reader (PerkinElmer, Integration time 0.5 sec/well). To investigate reaction kinetics ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT and signal amplification for 8 min using Cy3 (NEL704A001KT, Perkin Elmer) 1:50 in amplification diluent ...
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bioRxiv - Bioengineering 2023Quote: ... and counted immediately three times for 5 minutes each on a Tri-Carb 2900TR Liquid Scintillation Counter (PerkinElmer). The average DPM from the three technical replicate readings was utilized as the readout ...
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bioRxiv - Immunology 2023Quote: ... Images for quantification were captured at various time points before the luminescent signal reached the saturation intensity and analyzed with Living image 4.3.1 software (PerkinElmer). Regions of interest (ROI ...
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bioRxiv - Cancer Biology 2024Quote: ... was added to all the wells of the plate at the final concentration of 0.2μM followed by 24-hour time-lapse microscopy using the OperaPhenix microscope (PerkinElmer). Image acquisition and quantification of POPO™-1 Iodide (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... was added in 5μl of media to the wells in quadruplicate as well as a negative (DMSO 0.1%) and positive control (BzCl 100μM) followed by time-lapse microscopy using the OperaPhenix microscope (PerkinElmer) for 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR products were validated using a LabChip GX II (Perkin Elmer) and sequenced using PlateSeq PCR Kits (Eurofins ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with oxidants at the indicated time in a 96-well plate (Perkin Elmer, Waltham, MT, USA). After removing the medium and washing with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... flash energy/light exposure high/medium and integration lag/counting time 400/400μs (Victor X4 multilabel plate reader, PerkinElmer).
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bioRxiv - Cancer Biology 2020Quote: ... Time-lapse movies and snapshot images were generated and analyzed by Harmony High-Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Cancer Biology 2021Quote: ... Slides were washed 3 times in PBS and incubated with tyramide Cy3 1:150 in amplification buffer (Perkin Elmer) for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times in TBST prior to incubation in ECL solution (Western Lightning Plus, Chemiluminescent Substrate, PerkinElmer) for 5 min and membrane development.
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bioRxiv - Biochemistry 2019Quote: ... Plates were then washed 6 times with PBST and buffer containing PBS and 0.2 µg/ml Eu3+-streptavidin (PerkinElmer) and incubated at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... The time-resolved fluorescence resonance energy transfer (TR-FRET) signals were detected by an EnVision multilabel plate reader (PerkinElmer) with the emission window ratio of 665 nm over 620 nm under 320 nm excitation ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were then rinsed in TBST 3 times and treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA). Membranes were exposed to GE Healthcare Hyperfilm ECL (28906839 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were washed three times with PBS and bioluminescence measured (luciferin 0.3 mg/mL, Xenogen IVIS 50; Perkin Elmer). We used 24-well transwell inserts and plates (5 μm 29442-118 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed 3 times with wash buffer (PBS + 1 mM CaCl2 + 0.1% BSA) before adding Microscint-20 (PerkinElmer) for tritium concentration measurement using a TriLux MicroBeta2 plate reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: The time series images acquired using the Opera Phenix were analysed using the Harmony phenoLOGIC Software (v4.9; Perkin Elmer). The total fluorescence was measured and averaged across six fields per well ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... OPAL kit (PerkinElmer) fluorophores were used and prepared according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were used as template to incorporate radioactive 32P-dCTP (Perkin Elmer) using the High Prime kit (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed in TBST at least 3 times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839).
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bioRxiv - Cancer Biology 2020Quote: ... the two replicates of the 6 h treatment time point for the H1299 cell line were acquired on an equivalent Opera Phenix imaging system (PerkinElmer). Quantitative analysis of the data was carried out using the metrics and analysis methods detailed below ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pulse-labeled for the indicated times with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). After the pulse ...
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bioRxiv - Plant Biology 2022Quote: ... STR16 and STR18 to oxidize roGFP2 was analyzed in vitro by ratiometric time-course measurements on a fluorescence plate reader (EnSight multimode plate reader, PerkinElmer) with excitation at 400 ± 10 and 480 ± 10 nm and detection of emitted light at 520 nm with a bandwidth of 10 nm ...
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bioRxiv - Microbiology 2021Quote: ... The time-course measurements (Ex/Em=320/420 nm) were performed in the EnSpire multi-mode plate reader (Perkin Elmer). The initial linear regions in Fig ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were then rinsed in TBST three times and then treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839 ...
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bioRxiv - Cancer Biology 2022Quote: ... Brain tumors were detected and monitored over time using Fluc bioluminescence imaging using a Xenogen IVIS 200 imaging system (PerkinElmer) following intraperitoneal (i.p. ...
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bioRxiv - Molecular Biology 2020Quote: ... The signals of time-resolved fluorescence resonance energy transfer (TR-FRET) assay were measured in an Envision plate reader (PerkinElmer) with a TR-FRET mode (Ex=320 ...
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bioRxiv - Immunology 2021Quote: ... The inguinal LNs were extracted for imaging DLN and were imaged at indicated time points post injection using an in vivo imaging system (IVIS) Spectrum (PerkinElmer). The fluorescence imaging data were analyzed by Living Image software (PerkinElmer).
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were washed 5 min 3 times and developed using enhanced chemiluminiscence reagent (Western Lightning Plus-ECL; Perkin Elmer). The following primary antibodies were used ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times in 1 x PBS and imaged using the Opera Phenix high-content microscope (Perkin Elmer) at 63x magnification.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and imaged using the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). A minimum of nine fields were acquired per well using 20x water immersion objectives in confocal mode ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and imaged using the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). A minimum of nine fields were acquired per well using 63x water immersion objectives in confocal mode ...
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bioRxiv - Biochemistry 2023Quote: ... The hydrolysis reaction was then measured by absorbance at 630nm (read time of 0.1s per well, Victor 3 from Perkin Elmer) after incubating with malachite green reagent for a minimum of 8min under agitation.
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bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended and seeded as mentioned before in either 96- well (NRG1, EREG, AREG supplementation experiments,) or 384-well (time resolved EGF/NRG1 titration, PerkinElmer) imaging plates ...
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bioRxiv - Immunology 2023Quote: ... Signals of time resolved-fluorescence resonance energy transfer (TR-FRET) were collected by an EnVision 2105 multimode plate reader (PerkinElmer). Intracellular cAMP levels were calculated as instructed by the manufacturer.
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bioRxiv - Biochemistry 2024Quote: ... signals from time-resolved fluorescence resonance energy transfer (TR-FRET) were recorded using an EnVision 2105 multimode plate reader (PerkinElmer). The determination of intracellular cAMP levels was based on the TR-FRET signals of the samples in comparison to cAMP standards.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... signals of time resolved-fluorescence resonance energy transfer (TR-FRET) were detected by an EnVision 2105 multimode plate reader (PerkinElmer). Intracellular cAMP levels were calculated according to the TR-FRET signals of the samples and cAMP standards.
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bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were then transferred to GeneScreen Plus Hybridization Transfer Membrane (Perkin Elmer) and detected by hybridization using a non-radioactive method as described in Tomé et al.30.
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Cancer cell growth was monitored in vivo at the indicated times by retro-orbital injection of 100 μl of 15 mg/mL luciferin (Perkin Elmer) dissolved in 1X PBS ...