Labshake search
Citations for PerkinElmer :
301 - 350 of 503 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: Chemiluminescence detection of the proteins was performed using Western Lightning ECL-Plus (Perkin Elmer) and an Odyssey CLx device (LI-COR) ...
-
bioRxiv - Microbiology 2024Quote: ... Each collected sample was incubated with EasyTag EXPRESS-35S protein labelling mix (Perkin Elmer) at 23 µCi mL-l for 2 min at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... 50% and 25% MIA PaCa-2 cells) were grown in CellCarrier Spheroid ULA 96-well Microplates (6055330, PerkinElmer, Waltham, MA), as described previously ...
-
bioRxiv - Cell Biology 2020Quote: ... solid-state laser lines and a cooled 14-bit frame transfer EMCCD C9100-50 camera (Hamamatsu) and is run by Volocity (PerkinElmer). When imaging strains expressing the CRY2-Cdc42ΔCaaX and/or CRY2 systems ...
-
bioRxiv - Cancer Biology 2022Quote: Whole slide scans were captured at 10x with the PerkinElmer Vectra3 Slide Scanner and ~50 regions of interest (ROIs) per tumor chosen manually with PhenoChart (PerkinElmer). ROIs were imaged at 20x resolution and imported into InForm analysis software (PerkinElmer) ...
-
bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
-
bioRxiv - Biochemistry 2021Quote: ... Total radioactivity in each phosphorylation reaction was determined by mixing 50 µL aliquots of each reaction (in duplicate) thoroughly with 150 µL Microscint-40 (PerkinElmer) by pipetting the mixture at least 50 times ...
-
bioRxiv - Microbiology 2021Quote: ... and fluorescence at 355 nm excitation and 460 nm emission was monitored every 5 minutes for 50 minutes using an Envision plate reader (PerkinElmer). Rate of substrate cleavage was determined using linear regression of the raw data values obtained during the time course ...
-
Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
-
bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
-
bioRxiv - Cancer Biology 2022Quote: ... in 100-µL sterile PBS were injected into the left cardiac ventricle of female 5-week-old NSG mice as described previously (50) and in vivo imaging was performed (IVIS Spectrum, PerkinElmer) immediately to verify widespread seeding of tumor cells ...
-
bioRxiv - Cell Biology 2019Quote: ... solid-state laser lines and a cooled 14-bit frame transfer EMCCD C9100-50 camera (Hamamatsu) and is run by Volocity (PerkinElmer). When imaging strains expressing the OptoQ61L and/or Opto systems ...
-
bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
-
Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... 100 nM GBR12909 (to block binding to dopamine transporter) and 50 pM [125I]RTI-55 (NEX272, Perkin Elmer, Boston, MA) were added to the buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... Infection levels were inferred from the amount of luminescence in relative light units (RLUs) after adding 50 μL Britelite plus reagent (PerkinElmer) to 50 μL of media containing cell (i.e ...
-
bioRxiv - Immunology 2022Quote: ... This reaction was stopped by the addition of 50 ul of 2M H2SO4 prior to spectrophotometric analysis at 450 nm (PerkinElmer).
-
bioRxiv - Neuroscience 2022Quote: ... Drugs were added to the plates using a 50 nL solid pin tool attached to a Janus automated workstation (PerkinElmer), for a final screening concentration of 100 nM ...
-
bioRxiv - Neuroscience 2022Quote: ... 1.49- NA and an EM-CCD camera (C9100-50; Hamamatsu Photonics) under the control of Improvision UltraVIEW VoX system (PerkinElmer). Live cell imaging buffer (Invitrogen ...
-
bioRxiv - Developmental Biology 2023Quote: ... samples were washed 5x with TNT buffer and the second florescent signal was detected by incubating the samples in Cy5-TSA diluted 1:50 in Diluent buffer (PerkinElmer). The samples were washed at least 4x for 15 minutes each wash and then stained with Hoechst at 4°C overnight on a shaker before being put into Scale A2.
-
bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Gels were stained with Coomassie dye and bands containing mononucleosomes were excised (with serum albumin as a supporting lane marker) and incubated in a solution of 50% Solvable (PerkinElmer) and 50% water at 50°C for three hours ...
-
bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were mixed with the DNA absorption solution (for one sample: 50 uL 5 M NaCl, 15 ul magnetic beads (GMG-252-A-100mL – PerkinElmer), 250 uL 7.8 M guanidium chloride ...
-
bioRxiv - Immunology 2023Quote: ... Platinum (0.5ppt to 10ppb standards) and sulfur (50 ppb to 500ppb standards) standard curves were generated using single element standards (PerkinElmer) to quantitatively measure levels of platinum and sulfur ...
-
bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed in Maleic buffer and then PBS at room temperature prior to incubation with 50 µl amplification diluent with 1 µl Tyramide-Cy3 (Perkin Elmer) for 3 h at room temperature in the dark ...
-
bioRxiv - Molecular Biology 2021Quote: ... The re-collimated beam is divided by means of a 50/50 non-polarising beam splitter cube and is detected by two single-photon avalanche diode detectors (SPCM-AQR-13, Perkin Elmer). Time-traces of fluorescence intensity from the two detectors were cross-correlate to avoid after-pulsing artefacts ...
-
bioRxiv - Microbiology 2021Quote: ... a 150-μl volume of cells was resuspended and mixed by pipette with 50 μl of the luciferase substrate reagent BriteLite Plus (Perkin Elmer) in white 96-well plates ...
-
bioRxiv - Cancer Biology 2020Quote: ... Harmony high content analysis software was used to analyze the images and to count formation of spheres with diameter ≥ 50 µm (Version 4.8; PerkinElmer, Hamburg, Germany).
-
bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
-
bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
-
bioRxiv - Neuroscience 2019Quote: ... Detection was visualized with tyramide-488 1:50 in supplied amplification buffer from the TSA-Plus in situ kit (Perkin Elmer).
-
bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293T cells were seeded and 24 hours later transfected with 50 nM of siPOOLs targeting RPL24 or non-targeting siPOOLs as control (ON-TARGETplus siRNA, Horizon, Perkin Elmer) using HiPerFect transfection reagent (301705 ...
-
bioRxiv - Microbiology 2023Quote: ... collected water samples (50 mL) were immediately spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h under in situ natural illumination ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: WT or ARE-LUC expressing HepG2 were grown in either 24-well or 96-well plates to approximately 70% confluence when the medium was changed to serum-free medium supplemented with 100 nM 75Se (University of Missouri Research Reactor; specific activity of ∼500 Ci/g) or 50 uCi/ml 35S Met/Cys (Perkin Elmer). For drug or control treatments ...
-
bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
-
bioRxiv - Molecular Biology 2023Quote: ... the SPOT arrays were incubated in methylation buffer containing additionally 50 nM SETD6 and 0.76 µM labelled [methyl-3H]-AdoMet (Perkin Elmer Inc.) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 μl of media from each sample and 50 μl of the enzyme and substrate mix was pipetted in a well of the imaging plate (PerkinElmer, 6055300). The plate was briefly mixed and incubated for 1 h at RT avoiding exposure to light ...
-
bioRxiv - Neuroscience 2023Quote: ... The slides were thawed and fixed for two min with 0.1% paraformaldehyde in PBS at RT and then incubated in 50 pM I125-OVTA (2200 Ci/mmol; PerkinElmer; Boston, MA), a selective ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
-
bioRxiv - Microbiology 2024Quote: ... The filters were rinsed with 35 ‰ sodium chloride (NaCl) solution [50] and transferred into 7 mL scintillation vials containing a 6 mL scintillation cocktail (Ultima Gold ™ Cocktail, PerkinElmer). The radioactivity of the filters was determined using a Tri-Carb 3110TR liquid scintillation counter [51] ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Reagents for iodination of proteins were obtained from the following sources: Na125I (PerkinElmer, Waltham, MA), 1,3,4,6-tetrachloro-3α,6α-diphenyl-glycouril (Iodogen® ...
-
bioRxiv - Genetics 2022Quote: ... Proteins were detected using the Western Lightning Plus-ECL chemiluminescent substrate (Perkin Elmer, Cat #NEL103001EA) and autoradiograph film processed in an X-ray film developer.
-
bioRxiv - Cell Biology 2020Quote: ... HA-binding protein staining was achieved with 1/100 strepavidin HRP (Perkin Elmer, Massachusetts, USA) solution for 30 min at room temperature and 3,3’-diaminobenzidine (Liquid DAB + substrate Chromagen System ...
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... followed by incubation with 35S-Met/Cys (30 mCi/mL EXPRESS Protein Labeling Mix, PerkinElmer) for an additional 30 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Viral titer was determined using p24 ELISA test measuring viral capsid protein p24 (Perkin Elmer, performed in Biomedicum Functional Genomics Unit) ...
-
bioRxiv - Cell Biology 2020Quote: ... protein signals were detected via enhanced chemiluminescence (Western Lightning Plus ECL, Perkin Elmer, Waltham, MA) followed by exposure to film (CL-XPosure ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were detected with the chemiluminescence detection reagent Perkin Western Lightning plus ECL (Perkin Elmer) and visualized using a radiology film processor (Curix 60 ...
-
bioRxiv - Microbiology 2022Quote: ... in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
-
bioRxiv - Molecular Biology 2023Quote: ... in total volume of 18 μl using Protein A acceptor and Streptavidin donor beads (PerkinElmer). Varying amounts of the prefusion and postfusion form of RSV F protein in PBS supplemented with mucin (10 mg/L) ...