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Citations for PerkinElmer :
51 - 100 of 104 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [a32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [α32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... into female C57BL/6J (JAX strain #000664) mice and tumor burden was measured by bioluminescence (PerkinElmer IVIS Spectrum In Vivo Imaging System, D-luciferin PerkinElmer #122799). Data was analyzed using Living Image software.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer), and the other half was used for immunoblotting.
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL cell lysate was added to 3 mL of Ultima Gold scintillation liquid (Perkin Elmer #6013329). Samples were kept in the dark overnight and 3H-2DG uptake was measured the following day on a Hidex 300 SL scintillation counter ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mL of the cell lysate was counted in a liquid scintillation counter (TRI-CARB 4910TR, PerkinElmer) and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit ...
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bioRxiv - Microbiology 2021Quote: The cytotoxic effect of the wild-type and mutant strains were analyzed by live/dead cell assay using the High Content Screening (HCS) System (Operetta, Perkin Elmer, USA). At 12 h of post-infection ...
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bioRxiv - Microbiology 2023Quote: ... The 3280 compounds were tested in a final concentration of 10 µM with the rhlA-gfp [30] reporter strain in 96 wells microtiter dish (Black Isoplate®, Perkin Elmer). The cells were added in a final concentration of OD600=0.1 and final volume of 200 µl in ABGC medium and incubated at 37°C with shaken (200 rpm) ...
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bioRxiv - Cell Biology 2020Quote: ... Absorbance of the lysates was measured at 420 nm using a spectrophotometer (EnSpire Multimode Plate Reader, Perkin Elmer).
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were transferred into vials and measured using a gamma counter (Wallac 1470 Wizard, PerkinElmer, Waltham, MA, USA). The saturation assay was performed by adding 100 μL of binding buffer with increasing amounts of 125I-NDP-MSH to the cells in the presence or absence of 1 μM of unlabeled NDP-MSH.
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was quantified by measuring luciferase activity in cell lysates using Britelite Plus (Perkin Elmer, Richmond, California) and following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... 20 µL of the lysate was transferred in duplicate in a well of an OptiPlate-96 HB (PerkinElmer) and 100 µL of Luciferase Assay Reagent II was added in each well ...
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bioRxiv - Immunology 2023Quote: ... equal amounts of lysate (six replicates per condition) were spotted onto a 96-well DEAE filter mat (PerkinElmer) and the mat was dried at 60°C ...
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bioRxiv - Cell Biology 2023Quote: ... the rabbit reticulocyte lysate master mix was supplemented with EasyTag L-35-Methionine (4% final reaction volume; PerkinElmer). Canine rough pancreatic microsomes (cRMs ...
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bioRxiv - Cell Biology 2020Quote: ... Radioactivity was measured by liquid scintillation counting (100 μl of lysate in 3.9 ml of Emulsifier-Safe, Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: ... The supernatant and the cell lysate were added into separate scintillation tubes containing Ultima Gold LSC cocktail (Perkin Elmer) and the radioactivity was measured by a TriCarb 3100TR liquid scintillation counter (Perkin Elmer) ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated for 1 hour at room temperature before FRET was detected using an Envision plate reader (PerkinElmer). Emission of Lumi4TM-Tb cryptate was detected at 620 nm and emission of d2-conjugated IP1 at 665 nm ...
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bioRxiv - Microbiology 2022Quote: ... Reactions contained 10 μL rabbit reticulocyte lysate with 500 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes before being chased with 2 μl of 50 mg / mL unlabelled methionine ...
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bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
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bioRxiv - Cell Biology 2023Quote: ... by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC, PerkinElmer) for 1 h at 30 °C at 600 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Pathology 2024Quote: ... The fluorescence intensity (FI) of the medium and lysate was measured using an enzymatic calibrator (Perkin Elmer, Massachusetts, USA) at 469 nm excitation wavelength and 537 nm emission wavelength ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 ug of protein lysate was resolved by SDS-PAGE and transferred onto a Protran pure 0.2 micron nitrocellulose (Perkin Elmer). The membranes were incubated for 1h in 5% skimmed milk and probed with specific primary antibodies (Table S1 ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were prepared with Passive Lysis Buffer and Luciferase activity was measured on a VICTOR X3 plate reader (PerkinElmer). Luciferase assay values are represented in fold change of Fluc (pGL4.23 plasmid ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
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bioRxiv - Immunology 2020Quote: ... luciferase activities of 150 μl lysate transferred from each well to a 96-well black solid plate were measured by a luminometer (PerkinElmer). The 50% inhibitory dose (ID50 ...
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bioRxiv - Immunology 2021Quote: ... cell lysate was loaded onto a 96-well plate (Grenier-BioOne) and mixed with MICROSCINTTM-20 scintillation fluid (Perkin Elmer). Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Genomics 2022Quote: ... 20 µl of the lysate was transferred in duplicate in a well of an OptiPlate-96 HB (PerkinElmer, Waltham, MA) and 100 µl of Luciferase Assay Reagent II was added in each well ...
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the parental cell line ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µ g of lysate was used for the assessment of ATP concentration using an ATP Determination Kit (ATP lite assay, PerkinElmer) as described earlier (Miyoshi et al. ...
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the NP-2 parental cell line ...
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bioRxiv - Microbiology 2023Quote: ... The infectivity in each well was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). The percentage of inhibition was determined by comparing the infectivity with positive control wells without drug inhibition.
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bioRxiv - Microbiology 2024Quote: ... and the infectivity was determined by measuring the RLUs in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infection was considered positive if the RLU value was more than 5-fold higher than the background RLU value in parental NP-2 cell line ...
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bioRxiv - Microbiology 2024Quote: ... The infectivity in each well was determined by measuring the RLU in the cell lysate using the Britelite plus system (PerkinElmer). The percentage of Maraviroc inhibition was determined by comparing the RLU values with the positive control wells without Maraviroc inhibition ...
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bioRxiv - Cancer Biology 2020Quote: ... Both supernatant and lysate were moved to separate counting vials and mixed with 3 mL scintillation fluid (Ultima Gold #6013321, Perkin Elmer). 14C levels were measured in each sample using a Packard Liquid Scintillation Analyzer ...
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bioRxiv - Microbiology 2021Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infectivity was considered positive when the RLU value was at least 5-fold higher than the background RLU value in the parental NP-2 cell line ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Physiology 2022Quote: ... Radioactivity was measured on 150 or 250 μl of muscle lysate by liquid scintillation counting (Ultima Gold and Tri-Carb 4910 TR, Perkin Elmer) and related to the specific activity of the incubation buffer.
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bioRxiv - Immunology 2022Quote: ... 150 μl of lysate was transferred to white solid 96-well plates for the detection of luminescence using a microplate luminometer (PerkinElmer, Ensight). The positive well was determined as ten-fold relative luminescence unit (RLU ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
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bioRxiv - Genetics 2023Quote: ... was added in unison to the lysates and luminescence activity in counts per second were immediately recorded on a Victor X4 plate reader (Perkin Elmer). Using a multichannel pipet ...
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Microbiology 2024Quote: ... and the uptake of 14C(U) l-arginine was measured in the bacterial lysates using a liquid scintillation counter (Perkin Elmer).
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bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μL of lysate was added in triplicate to a 384-well white-walled plate (ProxiPlate-384 Plus, shallow-well microplate, PerkinElmer, Waltham, MA) and read on an EnVision plate reader (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: Coupled in vitro transcription and translation reactions were carried out using the Promega TnT® Coupled Reticulate Lysate System labelled with radioactive [35S] methionine (PerkinElmer Inc.) according to the manufacturer’s protocol ...