Labshake search
Citations for PerkinElmer :
1 - 50 of 291 citations for Yellow Fever virus Envelope protein sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
bioRxiv - Bioengineering 2024Quote: ... Vivo-Tag 680 XL (PerkinElmer #NEV11119) was used as a fluorescent dye to track idLFA1 and control murine serum albumin (MSA ...
-
bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
-
bioRxiv - Microbiology 2020Quote: ... All virus supernatant was quantified via p24 ELISA (Perkin Elmer).
-
bioRxiv - Immunology 2020Quote: ... Serum-virus mixtures were then transferred to 96-well plates (PerkinElmer), and 1.0×104 MDCK-SIAT1-PB1 cells51,92 were added into each well ...
-
bioRxiv - Cell Biology 2019Quote: ... Easy-Tag [35S]-Cys/Met was purchased from PerkinElmer Life Sciences ...
-
bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
-
bioRxiv - Immunology 2024Quote: ... the plates were centrifuged at 500 × g for 5 min and 20 µl of supernatant from each well was transferred to a 96 well DELFIA Yellow Plate (PerkinElmer) and combined with 200 µl of europium ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... pseudotyped virus treated hESC-derived cardiomyocytes in CellCarrier-96 Ultra Plates (PerkinElmer) were washed 3x with 100 μL PBS before complete submersion in 2-4% formaldehyde solution for 30 mins ...
-
bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
-
bioRxiv - Cell Biology 2020Quote: ... The alpha-glucosidase activity was determined by measuring the yellow-colored para-nitrophenol released from pNPG at 405 nm (EnVision Multimode plate reader, Perkin Elmer, USA). The results were expressed as the percentage of the blank.
-
bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) and by real time PCR ...
-
bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
-
bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) or by qPCR ...
-
bioRxiv - Cell Biology 2021Quote: ... and 100μl Easy Tag Express 35S Methionine/Cysteine (Perkin Elmer NEG 772002MC) at either 0 or 24h after transformation with a 6xMyc-Pex14 (pAMW-Pex14 ...
-
bioRxiv - Microbiology 2019Quote: ... WSN-GFP virus expression was detected by the Operetta-High Content Imaging System (PerkinElmer Inc.), and the percentage of GFP-positive cells were analyzed by Harmony4.1 software (PerkinElmer Inc.) ...
-
bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
-
bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
-
bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
-
bioRxiv - Bioengineering 2022Quote: ... Two avalanche photodiodes detect the fluorescent photons (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
-
bioRxiv - Immunology 2020Quote: ... The virus infectivity titers were determined by measuring the luciferase activity using Britelite luciferase substrate (PerkinElmer Inc.) in a luminometer (Victor X2 ...
-
bioRxiv - Microbiology 2022Quote: ... the virus was identified using liquid scintillation analyzer TRI-carb 2910 TR (Perkin Elmer, Waltham, Massachusetts, US). The virus containing fractions were collected and pelleted as earlier ...
-
bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
-
bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
-
bioRxiv - Cell Biology 2022Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
-
bioRxiv - Biophysics 2020Quote: ... The fluorescence was directed to a fiber coupled APD (SPCM-AQR-14-FC, Perkin Elmer), which was connected to a time-correlated single photon counting module (SPC830 ...
-
bioRxiv - Neuroscience 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
-
bioRxiv - Cell Biology 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
-
bioRxiv - Immunology 2021Quote: ... The luciferase signal was determined in live animals one day after virus injection and 10 minutes after intraperitoneal injection of 150 mg/kg D-luciferin (PerkinElmer) using the in vivo imaging system NightOWL II LB 983 (Berthold ...
-
bioRxiv - Neuroscience 2022Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
-
bioRxiv - Neuroscience 2019Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
-
bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
-
bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... we used the TTL signal from an avalanche photo diode (APD, SPCM-AQRH-13-FC, Perkin Elmer). We illuminated the APD with natural light ...
-
bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
-
bioRxiv - Immunology 2020Quote: ... The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2, PerkinElmer Life Sciences).
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
-
bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... and imaged onto the active area of two fibre-coupled avalanche photodiode detectors (APDs; Perkin Elmer, SPCM-AQRH-15-FC) using a cubic non-polarizing beam-splitter (Linos ...
-
bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
-
bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
-
bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
-
bioRxiv - Biophysics 2020Quote: ... a fast and mild filtration step is performed and the tag-PDZ concentrations were measured by capillary electrophoresis instrument (LabChip GXII, PerkinElmer, Massachusets, USA). A detailed protocol of how to run the holdup assay in an automatic way using liquid handling robots can be found in [29] ...
-
bioRxiv - Neuroscience 2023Quote: ... brains were incubated during 2 weeks at 37°C in presence of an AT8 antibody that specifically binds hyperphosphorylated tau (pSer202/Thr205/PSer208, produced at Janssen Pharmaceutica) and is directly labelled with a near-infrared fluorescent tag (PerkinElmer VivoTag 680XL). Brain samples were then dehydrated ...
-
bioRxiv - Biophysics 2019Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
-
bioRxiv - Bioengineering 2020Quote: MTs were transferred with a tip quickly coated in fetal calf serum (FCS) into a CellCarrier-384 Ultra Microplates (6057300, Perkin Elmer). They were then fixed in 4% PFA (P6148 ...