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Citations for PerkinElmer :
51 - 100 of 256 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
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bioRxiv - Molecular Biology 2023Quote: ... Generated AFC signal is detected after 15 min at RT using Ex/Em = 360/460 nM (Envision, PerkinElmer). Final assay concentrations ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 15 mg/mL (at 5 µL/g BW) of D-Luciferin (Perkin Elmer) and imaged ...
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bioRxiv - Neuroscience 2024Quote: ... Imaging was performed after 15 min with an Opera High Content Screening System (Perkin Elmer, Waltham, MA, USA). In the whole screening ...
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bioRxiv - Neuroscience 2021Quote: ... was added to the chamber for 30 min to inhibit transcription after which slices were transferred to fresh aCSF containing ~10 mCi/ml [35S] Met/Cys (Perkin Elmer) for an additional 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... with the exception that transcription reactions were performed in the presence of 0.25 mM GTP and 60 μCi of α-[32P]-GTP (Perkin Elmer).
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bioRxiv - Developmental Biology 2022Quote: Antisense RNA probes for in situ hybridization were prepared by in vitro transcription using Digoxigenin- or Dinitrophenyl-conjugated UTP (DIG, Roche 11277073910; DNP, PerkinElmer NEL555001EA) as previously described [56] ...
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bioRxiv - Physiology 2022Quote: ... 100 ng of PCR product was used in an in vitro transcription reaction with T7 polymerase (antisense) in the presence of 35S-uridine 5 triphosphate (Perkin Elmer) for radioactive in situ hybridisation.
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bioRxiv - Neuroscience 2021Quote: ... confocal micrographs of around 15 representative worms for each genotype were acquired using a spinning-disc confocal microscope (PerkinElmer). The Index of ATG-9 punctum at Zone 2 of AIY for each genotype was consistent across different confocal settings (data not shown) ...
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bioRxiv - Genetics 2019Quote: ... Membranes were washed with PBS-T for 15 minutes and the signal was developed by ECL Plus (Perkin Elmer) or ECL Prime (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... pH 7.0) and incubated for 15 minutes in pre-warmed uptake buffer that contained the radioactive tracer 65 ZnCl2 (PerkinElmer) and non-radioactive ZnCl2 (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Neuroscience 2022Quote: ... in a 15 mg/mL solution and imaged in an IVIS Lumina II In Vivo Imaging System (Perkin Elmer) at 10-20 minutes post-injection ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Cancer Biology 2024Quote: ... Mesothelial clearance was performed by seeding MeT5A-GFP cells (15) at the density of 1.3x 104 cells/well into 384-well CellCarrier plates (PerkinElmer), pre-coated with 10μg/ml collagen type I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620 ...
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bioRxiv - Biochemistry 2021Quote: ... and SL5 generated via in vitro transcription were treated with Quick Calf Intestinal Alkaline Phosphatase before labeling with [γ-32P]-ATP (Perkin Elmer) and T4 PNK ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... anti-cGMP antibody (PerkinElmer anti-cGMP antibody ...
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bioRxiv - Cell Biology 2020Quote: ... and medium was exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37°C on a spinning disc confocal system (Ultraview Vox, PerkinElmer) with a 100 × 1.4 NA oil-immersion objective ...
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bioRxiv - Cancer Biology 2019Quote: ... Luminescence imaging carried out every 2-3 days using an IVIS luminescence imaging system 10 min after mice were injected with 10 μl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer). Mice were sacrificed by CO2 asphyxiation followed by cervical dislocation if they reached AAALAC-defined endpoints ...
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bioRxiv - Cell Biology 2019Quote: ... and media exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37 °C on a spinning disc confocal system (Ultraview Vox; PerkinElmer) with a 100× 1.4 NA oil-immersion objective ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions containing 200 ng of mRNA and 0.1 mM of D-luciferin were carried out in 5 μL aliquots at 37°C for 15 minutes and the activity of in vitro synthesized luciferase was measured by VictorX5 (PerkinElmer) every 30 seconds.
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bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
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bioRxiv - Cancer Biology 2019Quote: ... mice were injected intra-peritoneally with XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate (15 mg/ml, 100ul/10g; Perkin Elmer). The mice were imaged with Xenogen IVUS Spectrum (Perkin Elmer) ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and imaged onto the active area of two fibre-coupled avalanche photodiode detectors (APDs; Perkin Elmer, SPCM-AQRH-15-FC) using a cubic non-polarizing beam-splitter (Linos ...
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bioRxiv - Immunology 2020Quote: ... and then re-fixed in formalin for 15 minutes followed by antigen retrieval as per the manufacturer’s instructions (Perkin Elmer). Blocking was performed for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... for lipid peroxidation for 15 min before addition of treatments and imaging in an Operetta high-content imaging system (PerkinElmer) in a temperature and CO2-controlled chamber at the indicated time intervals ...
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bioRxiv - Biophysics 2023Quote: ... Fluorescence was collected after a pinhole of 100 μm diameter size (P100D, Thorlabs) by an avalanche photodetector (SPCM-AQR-15, PerkinElmer) connected to an SPC-150 (Becker & Hickl ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
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bioRxiv - Developmental Biology 2023Quote: ... red and green fluorescence at 15 min intervals over 19h on an Opera Phenix™ High-Content Screening System (PerkinElmer) under standard culture conditions.
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bioRxiv - Microbiology 2023Quote: ... Samples were mixed with the DNA absorption solution (for one sample: 50 uL 5 M NaCl, 15 ul magnetic beads (GMG-252-A-100mL – PerkinElmer), 250 uL 7.8 M guanidium chloride ...
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bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Cancer cell growth was monitored in vivo at the indicated times by retro-orbital injection of 100 μl of 15 mg/mL luciferin (Perkin Elmer) dissolved in 1X PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... All biomarkers were measured in triplicate (intra-assay coefficient of variability [CV] < 15% in all cases) using an EnVision microplate reader (PerkinElmer, Finland) and calibrated to the protein concentration in the sample ...
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bioRxiv - Cell Biology 2019Quote: ... injected intraperitoneally with 150 mg/kg of D-Luciferin solution and imaged at 20 minutes post injection (for tumour monitoring) and 15 minutes post injection (for MSCs) using an IVIS Lumina (Perkin Elmer). Exposure times were optimised to ensure sufficient signal was obtained without saturation ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... bright field and digital phase contrast images of 10-15 fields per well were captured on an Operetta automated microscope (Perkin Elmer) at 30 min intervals for approximately 2 hours ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were subsequently washed three times with TBST (15 min each) before visualization of Chemiluminescence activity using Western Lightning ECL reagent (Perkin Elmer) in Chemidoc™ MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... washed twice with the same medium and pulse labeled for 15 min with 35S-Cys/Met (300 μCi, Cat. No. NEG072007MC, Perkin Elmer). After pulse ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were incubated with lids in place for 15 min at room temperature and then luminescence readings were taken using a ViewLux ultra HTS Microplate Imager (Perkin Elmer) with a 2 second exposure time per plate ...
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bioRxiv - Cell Biology 2023Quote: ... and an FF01-531/40-25 filter (Semrock) and was finally detected by an SPCM-AQRH-15 avalanche photodiode (Perkin Elmer). The time-correlated single photon counting module to register detected photon events to excitation pulses was SPC830 (Becker & Hickl) ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated in the dark for 10-15 minutes before luminescence was read on a Victor3V 1420 Multilabel Counter (Perkin Elmer).