Labshake search
Citations for PerkinElmer :
451 - 500 of 1280 citations for Total Phosphatase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
-
bioRxiv - Cancer Biology 2021Quote: ... High Content Screening system with confocal microplate imaging for readout and image analysis was performed using Columbus 2.4.0 (PerkinElmer, Waltham, Massachusetts, USA). The major measuring parameter of our assay was the K/EGFP level detected per cell and per well in relation to the total number of surviving cells after a 72 h treatment with compounds ...
-
bioRxiv - Microbiology 2022Quote: ... Cells (2 × 104 cells/well) were seeded into 96-well plates (Cell Carrier-96well Ultra microplate; PerkinElmer Inc., Waltham, MA, USA) and differentiated to macrophages with phorbol 12-myristate 13-acetate (10 ng/mL ...
-
bioRxiv - Microbiology 2022Quote: ... growth inhibition was determined by measuring absorbance at 595 nm in a Victor3 microplate reader (Perkin Elmer Inc., Waltham, MA, USA) [41] [42].
-
bioRxiv - Neuroscience 2022Quote: ... were added to each well and incorporation of 33Pi and quantification of incorporated cpm was determined with a microplate scintillation counter (Microbeta, Perkin Elmer).
-
bioRxiv - Microbiology 2023Quote: ... 100 μl of cell cultures from at least three biological replates were placed in a black 96-well plate in duplicate, and the fluorescence (485 nm excitation, 538 nm emission) was measured in a microplate reader (Enspire, Perkin Elmer). To measure the saeP1 activity during cell culture for 24 hr ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plates were incubated with lids in place for 15 min at room temperature and then luminescence readings were taken using a ViewLux ultra HTS Microplate Imager (Perkin Elmer) with a 2 second exposure time per plate ...
-
bioRxiv - Cell Biology 2023Quote: HT-1080 cells were seeded with a cell number of 1000 cells/well in 25µl cell culture media on PhenoPlate™ 384-well microplates (PerkinElmer, 6057308) with the MultiFlo Microplate Dispenser (BioTek ...
-
bioRxiv - Cell Biology 2023Quote: NMuMG cells expressing either EGFP-BIK-L61G or EGFP-PUMA4E were seeded (3,000 cells per well) in multiple wells (at least 3) of a 384-well microplate (CellCarrier-384 ultra, B128 SRI/160; Perkin Elmer) and allowed to grow for 24 h before staining with the nuclear dye DRAQ5 (5nM ...
-
bioRxiv - Biochemistry 2023Quote: ... The cells were finally resuspended in the same buffer and aliquoted into three plastic scintillation vials for counting using a MicroBeta2 microplate counter (Perkin Elmer).
-
bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... Incubations were done at room temperature (24°C) for 4h and the end-point fluorescence reading obtained with a multimode microplate reader (Envision or EnSight, Perkin Elmer) equipped with excitation 405 nm and emission 535 nm filters ...
-
bioRxiv - Cancer Biology 2023Quote: ... LN-229 cells were seeded at a density of 70,000 cells/well on poly-D-Lysine-coated ViewPlateTM-96 F TC 96-well black polystyrene clear bottom microplates (PerkinElmer, #6005182) in 100µl full medium ...
-
bioRxiv - Microbiology 2024Quote: ... Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer). OD600 of cell-free medium was removed from all cell density values ...
-
bioRxiv - Neuroscience 2024Quote: ... so that 25uL of each diluted compound was added per well of a 96-well plate (PhenoPlate 96-well microplate, PerkinElmer, 6055302) to make up desired concentrations.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the fluorescence transfer is measured at λex=337 nm and λem=620 and 665 nm using a microplate reader (Envison, Perkin Elmer). The cAMP concentration is determined by dividing the signal measured at 665 nm by that measured at 620 nm (ratio) ...
-
Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Assays were conducted in UniFilter-96 GF/B plates (PerkinElmer) with 1 ug of membranes per well in a final volume of 300 μl binding buffer consisting of 20 mM HEPES ...
-
bioRxiv - Neuroscience 2020Quote: Reagents for TR-FRET assays were purchased from Perkin Elmer. Reactions were performed with 50 nM ULight-Glycogen Synthase (Ser641/pSer657 ...
-
bioRxiv - Cell Biology 2021Quote: ... Assay was read in a multiplate reader Envision (Perkin Elmer). Results were fitted to a dose-response curve with variable slope and constraints of 0 and 100 for bottom and top ...
-
bioRxiv - Microbiology 2020Quote: ... An ATPlite Luminescence Assay System (PerkinElmer, Waltham, Massachusetts, United States) was used to test for cytotoxicity ...
-
bioRxiv - Cancer Biology 2020Quote: ... cellular ATP levels were measured using ATPlite Luminescence Assay (PerkinElmer). Data were normalized to the number of cells under DMSO conditions ...
-
bioRxiv - Cancer Biology 2020Quote: The ATP-monitoring ATPlite luminescence assay system from Perkin Elmer Inc ...
-
bioRxiv - Cancer Biology 2022Quote: ... then cell viability was measured using ATPlite Luminescence Assay (PerkinElmer).
-
bioRxiv - Microbiology 2021Quote: ... as determined with a PE Britelite Plus Assay (PerkinElmer, 6066769), and the results were read on a luminometer (PerkinElmer) ...
-
bioRxiv - Cell Biology 2020Quote: ... Luminescence ATP detection assay system (ATPlite) was purchased from PerkinElmer. For western blot analysis ...
-
bioRxiv - Biophysics 2022Quote: ... These assays were performed using LS 55 spectrofluorimeter (Perkin Elmer). Three consecutive spectra were averaged after blank correction for obtaining the final LS and ThT spectra.
-
bioRxiv - Cell Biology 2022Quote: ... dissolved in 1X Alpha-LISA assay buffer (Perkin Elmer, AL000F). Plates read by laser irradiation of donor beads at 680 nm generating chemiluminescent emission from the acceptor bead at 615 nm using EnVision Multimode Plate Reader (Perkin Elmer).
-
bioRxiv - Cancer Biology 2022Quote: ... or the LabChip RNA Pico Sensitivity Assay (PerkinElmer, Waltham, MA) before library generation ...
-
bioRxiv - Cell Biology 2022Quote: ... Assays were performed in white 384-well plates (Optiplates, PerkinElmer) and AlphaLISA readings made on a Pherastar FS (BMG Labtech ...
-
bioRxiv - Biophysics 2022Quote: ... All assays utilized 1 nM of radioligand ([3H]-Neurotensin, PerkinElmer). For dissociation assays ...
-
bioRxiv - Cancer Biology 2023Quote: ... Viability was assessed using ATPlite Luminescence Assay System (Perkinelmer, #6016949) and Calcein AM (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... then cell viability was measured using ATPlite Luminescence Assay (PerkinElmer).
-
bioRxiv - Biochemistry 2021Quote: ... and SL5 generated via in vitro transcription were treated with Quick Calf Intestinal Alkaline Phosphatase before labeling with [γ-32P]-ATP (Perkin Elmer) and T4 PNK ...
-
A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Total fluorescence was read on a multimodal plate reader (PerkinElmer Envision). The pH profiling assay was performed as above ...
-
bioRxiv - Molecular Biology 2021Quote: ... The plates were read at a wavelength of 460 nm using the VICTOR 3 V 1420 Multi-Label Microplate Reader (Perkin Elmer, USA). All reactions were carried out in triplicate using both antigenic peptides in separate experiments ...
-
bioRxiv - Cell Biology 2019Quote: ... Plates were left at room temperature for 10 min before imaging the ATP-coupled luminescence using a ViewLux microplate imager (PerkinElmer, Waltham, MA).
-
bioRxiv - Microbiology 2021Quote: ... Microbial growth was evaluated by turbidity of the medium by absorbance measurement (λ: 595nm) in a Victor3 microplate reader (1420 Multilabel Counter / Victor3 - Perkin Elmer), after 24 hours of incubation at 37 ° C.
-
bioRxiv - Cell Biology 2021Quote: RPE1-mAG-hGem cells were seeded at 1500 cells per well into 96-well Cell Carrier-96 ultra microplates (PerkinElmer, Woodbridge, ON) for at least 6 hr prior to treatment ...
-
bioRxiv - Cell Biology 2021Quote: RPE1-mAG-hGem cells were seeded at 1500 cells per well into 96-well Cell Carrier-96 ultra microplates (PerkinElmer, Woodbridge, ON) for at least 6 hr prior to treatment ...
-
bioRxiv - Cancer Biology 2020Quote: Resulting cells were ultra-concentrated in 10 μl of DPBS and plated onto wells of a CellCarrier-96 Ultra Microplates (PerkinElmer, Cat# 6055302). Plates were then centrifuged at 1800g for 10 minutes to allow the cells stick to the surface of the wells ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 μL of lysate was added in triplicate to a 384-well white-walled plate (ProxiPlate-384 Plus, shallow-well microplate, PerkinElmer, Waltham, MA) and read on an EnVision plate reader (PerkinElmer ...
-
bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
-
bioRxiv - Cell Biology 2021Quote: RPE1-mAG-hGem cells were seeded at 1500 cells per well into 96-well Cell Carrier-96 ultra microplates (PerkinElmer, Woodbridge, ON) for at least 6 hr prior to treatment ...
-
bioRxiv - Biophysics 2022Quote: ... The fluorescence of the supernatant was measured at 490 nm/535 nm (excitation/emission) using a Victor X5 Multilabel Microplate Reader (Perkin Elmer, USA). The data were fitted to a curve using a biexponential decay formula to obtain the half-life of the PEGylated formulations using the Origin 2022b software.
-
bioRxiv - Biochemistry 2022Quote: ... Detection reagents were incubated with reaction time points for 2 hours at room temperature in 384 well white microplates (PerkinElmer OptiPlate-384) then TR-FRET emission at 665 nm and 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
-
bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
-
bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
-
bioRxiv - Cell Biology 2024Quote: ... transfected cells were harvested using phenol red-free DMEM and seeded at a density of 1.0 × 104 cells/well onto CellCarrierTM-96 Ultra microplates (Perkin Elmer, Waltham, MA). Then ...
-
bioRxiv - Cancer Biology 2021Quote: ATP production was measured with the ATPlite Luminescence Assay System (PerkinElmer) according to manufacturer’s instructions.