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Citations for PerkinElmer :
451 - 500 of 671 citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cells were then washed with Krebs-Ringer bicarbonate HEPES (KRBH) buffer and then given 400 μL with KRBH buffer loaded with 0.8 μCi/well [3H]-2-deoxyglucose (PerkinElmer, NET549A001MC) and 0.5 mM unlabeled 2-deoxyglucose and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Genetics 2019Quote: ... PRMT7 wild-type and mutants were incubated with putative substrates in PBS with 2 μCi of S-adenosyl-[methyl-3H]methionine ([3H]AdoMet) (55-85 Ci/mmol, PerkinElmer) for 18 h at 26°C ...
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bioRxiv - Microbiology 2019Quote: ... and 500 µl were transferred to 2 ml Eppendorf tubes and spiked with 2.5 µCi of 32P-orthophosphoric acid (Perkin Elmer). The cultures were grown for two generations (OD600 0.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... was next added to the cultures and cells were labeled for 2 min with [2,8-3H]-adenine (NET06300 Perkin Elmer) at 0 ...
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bioRxiv - Bioengineering 2020Quote: Bioprinted SK-N-BE(2) spheroids were prepared as described earlier in a 96-well plate (Cell-Carrier, Perkin Elmer). After incubating the plate for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Microbiology 2023Quote: ... A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... The bioluminescent signal emitted by the disseminated tumors was measured at 7-day intervals for 2 months using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). The weight of the mice was recorded before each measurement.
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 12 hr following TNFα addition using the Opera Phenix Plus High-Content Screening System (PerkinElmer), with two biological replicates (n = 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 48 hr following TNFα addition using the Opera QEHS imaging system (PerkinElmer). Nuclear RELA and PCNA intensities were measured using Nuclitrack software (Cooper et al. ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 25-45 images were acquired on an Opera Phenix high-content imaging system (objective 20x, z-stacks of 2 μm, PerkinElmer). Image analysis was performed using Harmony imaging analysis software (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Physiology 2024Quote: ... or 120 nM of insulin in fresh serum-starvation medium for 30 min before adding 2 μL.mL-1 D-[14C(U)] glucose (NEC042B005MC; Perkin Elmer) for a further 90 min ...
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bioRxiv - Systems Biology 2019Quote: The enteroid transcriptomics libraries were constructed using the NEXTflex™ Rapid Directional RNA-Seq Kit (Perkin Elmer, 5138-07) using the polyA pull down beads from Illumina TruSeq RNA v2 library construction kit (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific libraries with unique barcodes were prepared using the NEXTFLEX Rapid Directional RNA-Seq kit 2.0 according to manufacturer’s instructions (Perkin Elmer). Libraries were pooled in equal molar amounts and sequenced on an Illumina HiSeq 2500 to obtain roughly 40 million reads per sample.
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bioRxiv - Molecular Biology 2020Quote: ... and then was probed with an antisense RNA Nluc probe that was transcribed with α-32P UTP (Perkin Elmer) as described (Arvola et al ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from standard cultures were prepared according to manufacturer’s protocol using the NextFlex Combo-Seq mRNA/miRNA kit (PerkinElmer), to capture both the mRNA and miRNA in the samples ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-10 ug RNA was separated in 1.8% formaldehyde agarose gel and transferred to a positively charged nylon overnight (GeneScreen plus, PerkinElmer). Subsequent steps were as for the small RNA blots.
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated from 150ng total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer, Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were processed according to the NEXTFLEX® Small RNA-Seq Kit v4 with UDIs (PerkinElmer, #NOVA-5132-32) with a 1 µg starting input and 12 cycles of PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... RRID: AB_2340590) were used as secondary antibody and blots were detected using ECL detection (PerkinElmer (PE), NEL105001EA) ...
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bioRxiv - Neuroscience 2020Quote: ... POD-conjugated antibody labeling of mRNA was proceeded with TSA Plus DNP signal amplification (Perkin Elmer) and a subsequently labeling with anti-DNP-KLH-488 antibodies (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Then those sections were stained with anti-MYC antibody with TSA plus fluorescein system (Perkin Elmer) followed by antigen retrieval to remove antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... After incubation in HRP-linked secondary antibody followed by ECL reagent (Western Lightning Plus-ECL (PerkinElmer) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... the membrane was probed with the appropriate antibodies and detected with Western Lightning Plus-ECL (PerkinElmer) using a Gbox (SYNGENE) ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were probed overnight at 4 °C with indicated antibodies and developed by chemiluminescence (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... the primary antibody was detected using Opal Polymer HRP Ms + Rb (PerkinElmer Opal Multicolor IHC system). The PerkinElmer Opal Multicolor IHC system was utilized to visualize antibody staining per manufacturer protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The Lmx1a antibody detection capability was improved using the Tyramide Signal Amplification kit (TSA, Perkin Elmer), in accordance with manufacturers instructions.
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bioRxiv - Physiology 2024Quote: ... Fluorescein was detected with a horseradish peroxidase-conjugated anti-fluorescein antibody (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized by using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
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bioRxiv - Neuroscience 2019Quote: ... 100µl from each of the wells was transferred to a vial and scintillation fluid (4ml) added (Optiphase Hisafe 2, PerkinElmer, UK). Radioactivity was measured using a Packard Tri-Carb 2900TR liquid scintillation counter (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: HUVEC cells were seeded in 6 cm2 plates (4 x 105 cells/well) and 8 hours after plating were pulsed for 2 hours with tritiated uridine (Uridine [5,6-3H]; Perkin Elmer; NET367001MC) at final concentration of 2 µCi/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the amount of tritiated acetate in the organic phase was measured by liquid scintillation counting for 2 min (Tri-Carb 2900TR, Perkin Elmer). Each sample was assayed in triplicate and counts were corrected for background by subtraction of counts obtained using whole blood samples devoid of cholinesterase activity and treated in a similar fashion ...
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bioRxiv - Plant Biology 2019Quote: The concentration of PINA was calculated by measuring optical density at 280 nm with a UV-spectrophotometer (Perkin Elmer Lambda 2) and by using an extinction coefficient of PINA (31.10 M−1 cm−1 or 2.376 g−1 L.cm−1) ...
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bioRxiv - Physiology 2021Quote: ... a longer time was required to achieve steady state) a single bolus of 12 μCi of [1-14C]-2-deoxy-D-glucose (Perkin Elmer) in 96 μl of saline was administered ...
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bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...