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Citations for PerkinElmer :
51 - 100 of 117 citations for Smooth Muscle Actin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Sections were subsequently blocked for 30 minutes in TNB Blocking Buffer (PerkinElmer) followed by incubation with an anti-human CD31 monoclonal antibody (1:30 ...
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Neuroscience 2022Quote: ... calcium imaging was recorded in both the vm1 and vm2 vulval muscles simultaneously and ratiometric analysis of the calcium recordings was performed in Volocity (PerkinElmer) to generate traces of calcium transients ...
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bioRxiv - Neuroscience 2021Quote: ... The cryosections were permeabilized and blocked in TSA blocking reagent (Perkin Elmer FP1020). Primary antibodies anti-BK 1:100 Antibodies Inc AB_224953 ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked by TN Blocking buffer (TSA Plus Fluorescence System; PerkinElmer, MA, USA). Primary antibodies were incubated for more than 18 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween20) and then blocked in TNT/block [0.5% blocking reagent (PerkinElmer FP1012)/TNT] for 1 hour at RT before ON incubation with anti-DIG (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PBS washing and treated with TNB blocking reagent (Perkin Elmer, FP1012) for 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in TNB (Blocking Reagent [Perkin Elmer; 0.05% in TN]; 30 min, RT), incubated with anti-DIG-POD antibody (Roche ...
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bioRxiv - Immunology 2021Quote: ... Peptide masses were initially predicted by ChemDraw Professional 15.0 (PerkinElmer, Massachusetts, USA) and compared to acquired masses after LC-MS measurements.
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bioRxiv - Microbiology 2021Quote: ... an equal volume of biotinylated SRC1 peptide/Europium-labeled streptavidin (Perkin Elmer) was added to biotynlated RORγ/APC-labeled strepavidin (Perkin Elmer) ...
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bioRxiv - Microbiology 2019Quote: ... The actin and ZIKV probes were randomly labeled with α32P dATP (Perkin Elmer) using a RadPrime labeling kit (Invitrogen).
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bioRxiv - Physiology 2022Quote: ... Radioactivity was measured on 150 or 250 μl of muscle lysate by liquid scintillation counting (Ultima Gold and Tri-Carb 4910 TR, Perkin Elmer) and related to the specific activity of the incubation buffer.
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bioRxiv - Microbiology 2021Quote: ... pre-incubated with blocking buffer (TNB) supplied in the TSA Plus kit (Perkin Elmer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were blocked in 3% hydrogen peroxide followed by TSA blocking buffer (Perkin Elmer #FP1012). Tissues were then incubated with rabbit Sera against MmuPV1 L1 (provided by Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 nM to 100 M using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte ...
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bioRxiv - Bioengineering 2019Quote: ... Aliquots of the peptide were deposited on a “Golden Gate” diamond ATR (PerkinElmer, USA). PBS was used as a background spectrum ...
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bioRxiv - Neuroscience 2021Quote: ... slides were blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% blocking reagent from PerkinElmer) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... and then blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% blocking reagent from PerkinElmer) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with TNB blocking buffer (from TSA Plus Cyanine 3 System, NEL744001KT, PerkinElmer, Waltham, MA, US) for 1 hr at RT and incubated with anti-DIG-POD (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed in PBS-T for 30 minutes and then blocked with 0.5% blocking buffer (PerkinElmer, FP120) for 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... muscle and femur samples were weighed and uptake of radioactivity was measured by a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). To determine the effect of unlabeled ligand on the tumor uptake ...
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bioRxiv - Neuroscience 2021Quote: ... placed in 0.5 % H2O2 in PBS for 30 minutes and then blocked with TNB solution (0.1M Tris-HCl, pH 7.5, 0.15M NaCl, 0.5% blocking reagent from PerkinElmer, NEL701A001KT) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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bioRxiv - Neuroscience 2020Quote: ... 3% TritonX-100) and TNB (0.1 M Tris-HCl, 0.15 M NaCl, 0.5% Perkin Elmer blocking reagent) buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were blocked by incubation with Tris-NaCl blocking buffer (TNB) (0.1 M Tris-HCl, pH 7.5, 150 mM NaCl, and 0.5% w/v blocking reagent, PerkinElmer) for analysis of Podoplanin ...
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bioRxiv - Developmental Biology 2022Quote: ... and blocked for 1 hour in TNB buffer (0.1M Tris-HCl, pH 7.5, 0.15M NaCl, 0.5% Blocking reagent [PerkinElmer FP1020]) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Compound plates containing synthetic peptides in DMEM/BSA were placed in a MicroBeta LumiJet luminometer (PerkinElmer). After loading ...
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bioRxiv - Molecular Biology 2021Quote: Peptide or small molecules stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Reactions were performed with 50 nM ULight-Glycogen Synthase (Ser641/pSer657) peptide (Perkin Elmer #TRF0131-M), 15 μM ATP ...
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bioRxiv - Molecular Biology 2022Quote: Peptide-or small molecule-stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of peptide remaining bound was measured using scintillation counting in Ultima Gold (Perkin Elmer). Experiments were performed a minimum of three times to generate an overall mean and standard deviation ...
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bioRxiv - Biochemistry 2022Quote: ... The structures of NTV and the substrate peptide were drawn in ChemDraw 20.0 (Perkin Elmer, USA) and optimized to generate 3D conformations using the LigPrep module in Maestro.
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bioRxiv - Cancer Biology 2021Quote: ... The sections were then incubated in a Tris–NaCl-blocking buffer (TNB-buffer, FP1020, PerkinElmer, Waltham, MA, USA) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... For immunofluorescence staining sections were blocked with TNB blocking buffer (0.1 M Tris-HCl, pH 7.5; 0.15 M NaCl; 0.5% (w/v) blocking reagent (PerkinElmer, Cat # FP1020) for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% PerkinElmer TSA blocking reagent) 30 min and incubated in primary antibodies overnight ...
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bioRxiv - Bioengineering 2020Quote: ... tissue was incubated for at least 30 minutes in blocking solution (TNB, Perkin Elmer, with 0.1% Triton X-100) and if a mouse primary ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Genetics 2019Quote: ... The slides were blocked in TNB buffer (0.1 M Tris-HCl pH 7.5, 0.15 M NaCl, 0.5% Blocking Reagent; PerkinElmer, Waltham, MA, USA) at RT for 45 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... Dehydrated coverslips were overlaid with 0.3 μg/ml TAMRA–OO-(CCCTAA)3 or 0.3 μg/mL Alexa 488–OO-(CCCTAA)3 telomeric PNA probe (Panagene) in PNA hybridization solution (70% deionized formamide, 0.25% (v/v) NEN blocking reagent (PerkinElmer), 10 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Substrate peptides or nucleosomes were mixed in a solution containing 9µM tritiated S-Adenosyl Methionine (SAM, Perkin Elmer) cofactor ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... 2.7 mM KCl with 0.2% Triton X-100 and then blocked by shaking for 60 min in TSA Blocking Reagent (Perkin Elmer). Sections were left shaking for 1-3 nights at 4°C in primary antibodies ...
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bioRxiv - Biochemistry 2021Quote: In vitro peptide-based Aur A assays were carried out using a Caliper LapChip EZ Reader platform (Perkin Elmer), which monitors real-time phosphorylation-induced changes in the mobility of a fluorescently labelled Kemptide peptide substrate (5’-FAM-LRRASLG-CONH2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...