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Citations for PerkinElmer :
1 - 50 of 117 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE gels were transferred to PVDF membranes (Perkin Elmer). Membranes were blocked in 1x PBST containing 5% (w/v ...
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bioRxiv - Evolutionary Biology 2023Quote: Western blotting was performed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western Lightning Plus-ECL (PerkinElmer) as previously described 50 ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were resolved by 12% SDS-PAGE and dried gels were exposed to a Cyclone Plus Storage Phosphor Screen (PerkinElmer). The screen was scanned using a Typhoon TRIO Variable Mode Imager (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... proteins were further analyzed by SDS-PAGE (LabChip™ GXII, Perkin Elmer). Peptides ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 ug of protein lysate was resolved by SDS-PAGE and transferred onto a Protran pure 0.2 micron nitrocellulose (Perkin Elmer). The membranes were incubated for 1h in 5% skimmed milk and probed with specific primary antibodies (Table S1 ...
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bioRxiv - Cell Biology 2019Quote: ... loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit; PerkinElmer). For Flag immunoprecipitations ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then separated with SDS-PAGE and detected by phosphor imaging (Cyclone Plus storage phosphor system, Perkin Elmer).
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bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then solubilized with 200μL of 1% SDS 0.2N NaOH and transferred to a scintillation vial with 5mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339). Radioactivity was measured with the TopCount scintillation counter (Perkin Elmer) ...
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bioRxiv - Immunology 2019Quote: ... Results were obtained using the SDS Software (Perkin Elmer) and evaluated using Excel (Microsoft) ...
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bioRxiv - Cell Biology 2020Quote: ... Single oocytes were transferred into Pico Prias scintillation vials (Perkin Elmer) and lysed in 200 μl 5 % SDS ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cells were manual tracked using Volocity software (V6.1.1, Perkin Elmer) for the course of the experiment ...
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bioRxiv - Bioengineering 2019Quote: ... Agarose gels were imaged by a gel-doc imaging system (Geliance 1000, PerkinElmer, USA) imaging system ...
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bioRxiv - Biophysics 2022Quote: ... followed by detection with SPCM-AQRH-14-TR single-photon avalanche diodes (PerkinElmer).
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bioRxiv - Biochemistry 2023Quote: ... microfluidic CE-SDS platform and a ProteinEXact Reagent Kit (Perkin Elmer, CLS150466) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel was treated with En3hance (Perkin Elmer), dried and exposed on film.
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bioRxiv - Physiology 2023Quote: ... 5 mL of Insta-gel Plus (PerkinElmer) was added ...
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bioRxiv - Synthetic Biology 2019Quote: ... Single-cell fluorescence was then analyzed by flow cytometry using a GALLIOS cytometer (Perkin Elmer) or with a MACSQuantTM VYB cytometer (Miltenyi Biotec GmbH) ...
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bioRxiv - Biophysics 2021Quote: ... The fluorescence was detected with two single-photon avalanche diode detectors (τ-SPAD, Perkin Elmer) using Time-correlated Single Photon Counting with the TimeHarp 200 board (HydraHarp 400 ...
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bioRxiv - Neuroscience 2019Quote: ... 5% SDS) with 25 ng of radioactively labeled probe ([α-32P]-dGTP, PerkinElmer) according to manufacturer instructions (Random Primers DNA Labeling System ...
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bioRxiv - Molecular Biology 2020Quote: ... The destained gels were soaked in EN3HANCE (PerkinElmer) according to the manufacturer’s instructions and visualized by fluorography ...
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bioRxiv - Plant Biology 2019Quote: ... agarose gel electrophoresis and via LabChIP GXII (PerkinElmer) analysis ...
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bioRxiv - Biophysics 2020Quote: ... Single-photon avalanche diodes were used for detection (SPCM-AQR-15, PerkinElmer, or τ-SPADs, PicoQuant). All experiments involving freely diffusing molecules were performed in µ-slide sample chambers (ibidi ...
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bioRxiv - Neuroscience 2021Quote: ... Image analysis scripts for single-cell analysis were developed using Acapella® Studio 5.1 (PerkinElmer Inc.) and the integrated Acapella® batch analysis as part of the Columbus® system.
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bioRxiv - Neuroscience 2023Quote: ... 25 single-plane images were acquired using a high-content screening system (Opera Phenix, Perkin Elmer) using a 40x water immersion objective (NA 1.1) ...
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bioRxiv - Physiology 2023Quote: ... Automated (unbiased) confocal single-cell fluorescence measurement was performed by Operetta CLS™ (Operetta– Perkin Elmer) at 40× magnification.
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bioRxiv - Systems Biology 2023Quote: ... were taken in a single plane using an automated spinning disk confocal microscope (Evotec Opera, PerkinElmer) with a 60x water-immersion objective.
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bioRxiv - Cancer Biology 2019Quote: ... stained single-fluorescence and unstained tissue was recorded with the Nuance Multispectral Imaging System (Version 3.0.2, PerkinElmer). A spectral library was built from the extracted spectra to enable the quantitative separation and spectral unmixing ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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bioRxiv - Biophysics 2023Quote: ... USA for the green channel) and focused onto the single photon detectors (SPCM, AQR 14, PerkinElmer, USA). A custom LabVIEW program was used for data acquisition.
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bioRxiv - Biochemistry 2019Quote: ... Gel was Coomassie stained and soaked in EN3HANCE (PerkinElmer). Dried gel was then exposed to film for one week at −80°C ...
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bioRxiv - Cancer Biology 2021Quote: ... For single cell invasion analysis spheroids in the above conditions were imaged using an Opera Phenix (Perkin Elmer) with a 5x (need NA ...
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bioRxiv - Synthetic Biology 2021Quote: Single-cell fluorescence of the samples was then analyzed by flow cytometry using a GALLIOS cytometer (Perkin Elmer) or with a MACSQuant™ VYB cytometer (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... Single-color FISH was developed by a TSA-Plus TMR System according to the manufacturer⍰s protocol (PerkinElmer) using a digoxigenin-labeled probe and an anti-dig-HRP antibody (Roche Applied Science) ...
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bioRxiv - Microbiology 2021Quote: ... of which one was [5’-32P] single-end labelled with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol-1) and T4 polynucleotide kinase (Thermofisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Images are reported as extended z-projections or a single plan (RNA ISH) collected using Volocity (Perkin Elmer) or Zen (Carl Zeiss ...
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bioRxiv - Genomics 2021Quote: ... Samples were treated with a single probe at a time and in combination with TSA Plus Cyanine5 (PerkinElmer) at a dilution of 1:1500 ...
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bioRxiv - Bioengineering 2022Quote: ... Pellets were resuspended in water and analyzed with a NexION 2000B single quadropole ICP-MS (PerkinElmer, Waltham, MA). A Meinhard nebulizer was used with a cyclonic glass spray chamber for the introduction of the sample ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in water and analyzed with a NexION 2000B single quadropole ICP-MS (PerkinElmer, Waltham, MA). A Meinhard nebulizer was used with a cyclonic glass spray chamber for the introduction of the sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Dried gels were exposed to a Storage Phosphor Screen (PerkinElmer) and the screen scanned in a Typhoon FLA7000 using phosphor autoradiography mode ...
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bioRxiv - Molecular Biology 2021Quote: ... Dried gels were exposed to a Storage Phosphor Screen (PerkinElmer), the screen was then scanned in a Typhoon FLA7000 using the phosphor autoradiography mode ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Images of single stained tissues for each reagent were used to build spectral libraries of the single dyes by using the Nuance Multispectral Imaging System (Version 3.0.2, PerkinElmer Inc.). These spectral libraries were used to unmix the original multispectral images obtained with the Vectra imaging system (Suppl ...
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bioRxiv - Cancer Biology 2023Quote: ... Multispectral images were unmixed using spectral libraries built from images of single stained tissues for each reagent using the inForm Advanced Image Analysis software (inForm 2.4.6; PerkinElmer/Akoya). A selection of 10-15 representative multispectral images spanning all nine tissue sections was used to train the inForm software (tissue segmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... a library from spectral peaks emitted by each fluorophore from single stained slides was created with the Advanced Image Analysis software (InForm 2.4, PerkinElmer) and used for spectral unmixing of multispectral images allowing for identification of all markers of interest ...