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Citations for PerkinElmer :
301 - 350 of 614 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... in total volume of 18 μl using Protein A acceptor and Streptavidin donor beads (PerkinElmer). Varying amounts of the prefusion and postfusion form of RSV F protein in PBS supplemented with mucin (10 mg/L) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection from the membranes was performed with Western Lightning ECL Pro substrate (PerkinElmer NEL121001EA).
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was detected using the Western Lighting Plus-ECL substrate from PerkinElmer (Waltham, MA) on a G:BOX Chemi XX6 gel doc system (Syngene) ...
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bioRxiv - Immunology 2024Quote: ... The target proteins were visualized by Western Lightning PLUS-ECL or Ultra kit (Perkin Elmer). The images were acquired by Azure Biosystems C300 or Thermo Fisher Scientific iBright FL1000 ...
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bioRxiv - Physiology 2020Quote: ... TSA® Plus fluorophore for channel 2 (cyanine 3, PerkinElmer; 1:1000; 30 min), HRP blocker (15 min) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μM mononucleosomes and 5.0 μM S-[methyl-14C]-adenosyl-l-methionine (PerkinElmer, #NEC363050UC) in the presence or absence of stimulatory or control peptides in concentrations as indicated in the text ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... which contains inhibitors and 1 μCi D-[2-3H]-glucose (23.4 Ci/mmol, PerkinElmer). After 30 s’ reaction ...
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bioRxiv - Physiology 2020Quote: ... 0.35 ml of transport solution with 0.5 µCi/ml [3H]2-deoxy-glucose (PerkinElmer) was added to the cells for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: The oligonucleotide substrates (Supplementary Table 2) were 5’-labelled with [γ-32P]ATP (PerkinElmer) and T4 polynucleotidyl kinase (PNK ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL 10X T4 PNK buffer and 2 μL [γ-32P] ATP (Perkin Elmer). The volume was adjusted to 10 μL with water and incubated at 37°C for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... a bolus injection of 2-[1,2-3H(N)]deoxy-D-glucose (PerkinElmer, Boston, Massachusetts) (0.4 μCi/g body weight ...
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bioRxiv - Cell Biology 2023Quote: ... The colorimetric reaction was read by Wallac 1420 Victor 2 (Perkin Elmer, Waltham, MA) or Synergy H1 microplate reader (BioTek ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then pulsed with 2.20 MBq per sample with Express Protein Labeling Mix (Perkin Elmer) and incubated for 2.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the radiolabeled proteins were detected and quantified using a Cyclone Plus Phosphor Imager system (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... Cultures were labeled with 80 µCi/mL [35S] EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer) for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and protein expression signals were detected using a chemiluminescence detection kit (Perkin Elmer, Waltham, MA, USA) and visualised using a chemiluminescence imaging system (Fusion Solo S ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using Western Lighting Plus ECL detection reagent (Perkin Elmer, Waltham, MA, USA) and imaged with the ChemiDoc TouchTM system (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... was added for 2 minutes before reading with a PerkinElmer Envision instrument (PerkinElmer, Waltham, MA). 50% neutralization titer (ID50 ...
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bioRxiv - Neuroscience 2021Quote: ... d(CH2)5 [Tyr(Me)2,Thr4,Orn8,(125I)Tyr9-NH2] (125I-OVTA, PerkinElmer, Inc.). An adjacent series of slides ...
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bioRxiv - Cancer Biology 2020Quote: ... Multiplex stained slides were imaged using the Vectra Multispectral Imaging System version 2 (Perkin Elmer). Filter cubes used for multispectral imaging were DAPI (440–680 nm) ...
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bioRxiv - Microbiology 2020Quote: ... 100 pmol of DNA was incubated with 2 μL 5mCi [γ32-P]ATP (Perkin Elmer) and 4 μL T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Cancer Biology 2022Quote: ... PRKACA inhibiting activity was screened using the EZ Reader 2 electrophoretic mobility shift platform (PerkinElmer). PRKACA enzyme (0.007 ng/mL ...
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bioRxiv - Biochemistry 2021Quote: ... The washed sepharose was then directly added to 2 mL Ultima GoldTM scintillation cocktail (PerkinElmer). Radioactivity was read by a Tri-Carb 2910TR scintillation counter (PerkinElmer) ...
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bioRxiv - Physiology 2020Quote: ... i.p.) and a trace amount of [1-14C]2-deoxy-D-glucose (10 μCi; PerkinElmer). After 40 min ...
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bioRxiv - Neuroscience 2020Quote: ... Radioactivity of the filter paper was measured in a Microbeta 2 Scintillation Counter (Perkin Elmer) after addition of 3 mL Ultima-Gold Scintillation Fluid ...
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bioRxiv - Molecular Biology 2022Quote: ... Luciferase measurements were performed every 1 to 2 hours on an EnVision plate reader (PerkinElmer). Results were analysed by employing python (v3.8.5) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 pmol of DNA was incubated with 2 μL 5 mCi [γ32-P]ATP (PerkinElmer) and 4 μL T4 PNK (New England BioLabs ...
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bioRxiv - Physiology 2023Quote: ... ip) and a trace amount of 2-deoxy-D-[1-14C] glucose (10 µCi; PerkinElmer). Forty minutes later ...
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bioRxiv - Microbiology 2023Quote: ... Infected cells were lysed 2 days after infection with a britelite plus (PerkinElmer, Cat#6066769), and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Physiology 2024Quote: ... Filters were dissolved in 2 ml liquid scintillation fluid (Filter Count, PerkinElmer, Waltham, MA, USA) and counted ...
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bioRxiv - Cell Biology 2019Quote: ... detection of proteins from the membranes was performed with Western Lightning ECL Pro solution (Perkin Elmer, #NEL121001EA).
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bioRxiv - Immunology 2021Quote: Data processing was done with Elucidator software (Protein Expression Data Analysis System (Ver. 4.0.0.ror2.31, Ceiba Solutions/ PerkinElmer) for label-free quantitation based on comparison of MS1 feature volume ...
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bioRxiv - Immunology 2019Quote: ... Cells were then distributed (80 µg of proteins per well) in a 96-well microplate (Optiplate, PerkinElmer). BRET2 between RLuc8 and GFP10 was measured 1 min after addition of 5 µM coelenterazine 400a/Deep blue C (Gentaur) ...
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bioRxiv - Cell Biology 2019Quote: ... eluted proteins were incubated overnight at 4 °C and measured with a microplate reader analyzer (EnVision, PerkinElmer). Excitation spectra were obtained collecting emission at 515 ± 15 nm ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Cell Biology 2022Quote: ... For the host protein response screen 10,000 cells/well were seeded on 96 well microplates (Perkin Elmer, CellCarrier-96 Ultra Microplates ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation of yeast cells (82) and in vivo labeling of iron-binding proteins with 55FeCl3 (Perkin Elmer) and immunoprecipitation of 55Fe/S proteins and scintillation counting were performed as published for yeast (59 ...
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bioRxiv - Cancer Biology 2024Quote: Leucine incorporation into proteins was assayed using 3H-Leucine (cat. # NET460001MC) obtained from Perkin Elmer (Waltham, MA), as described in a previous publication [33].
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bioRxiv - Molecular Biology 2021Quote: Groups of five oocytes were incubated with 0.5 mCi/ml EasyTag EXPRESS 35S protein labeling mix (PerkinElmer, NEG772007MC) in 100 µl complete OR-2 buffer at 23°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the separated proteins were electrophoretically transferred to a polyvinylidene difluoride (PVDF) membranes (NEF1002001PK, PerkinElmer, Waltham, MA, USA) by 400 mA for 75 minutes ...
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bioRxiv - Microbiology 2019Quote: ... and a final concentration of 125 µMci/mL of EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer, USA). Concentrations used for incubation correspond to 100- ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Streptavidin Donor beads (Cat # 6760002) and Protein A acceptor beads (Cat # AL101) were acquired from PerkinElmer (Waltham, MA).
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were detected by western blot analysis by using an enhanced chemiluminescence system (Western Lightning– ECL, PerkinElmer). Antibodies used were specific for PTEN (Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: ... 2020 submitted): namely in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
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bioRxiv - Neuroscience 2022Quote: ... 25-30 μg of membrane protein was combined with a fixed concentration (0.57-5.18 nM) of 3H-diprenorphine (PerkinElmer) and concentration curves of KUNB106 or positive control (naloxone for mu and delta ...