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Citations for PerkinElmer :
251 - 300 of 315 citations for Recombinant Mouse Icam1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Immunoreactive proteins were detected with either anti-mouse or anti-rabbit horseradish peroxidase-coupled secondary antibody followed by detection with enhanced chemiluminescence (PerkinElmer).
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
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bioRxiv - Bioengineering 2022Quote: ... cryogels were administered into the dorsal flank of an anesthetized mouse and the fluorescent intensity of the Cy5-HA cryogel was quantified using an IVIS spectrometer (PerkinElmer) at predetermined timepoints and analyzed using LivingImage software (PerkinElmer) ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were injected IP with luciferin (5 mg/mouse; Gold Technologies) and then imaged on a Xenogen IVIS Spectrum imager (PerkinElmer) 10-15 minutes later for 60 seconds ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were injected IP with luciferin (5 mg/mouse; Gold Technologies) and then imaged on a Xenogen IVIS Spectrum imager (PerkinElmer) 10 minutes later ...
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bioRxiv - Neuroscience 2020Quote: Phospho-Syk level from WT mouse primary microglia cultures were determined using AlphaLISA SureFire Ultra kit (PerkinElmer, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... injection of the RediJect inflammation probe (150ul/mouse, 200mg/kg) animals were imaged using the IVIS Spectrum imaging system (PerkinElmer). Bioluminescence images were acquired for 5min with F/stop=1 and binning factor = 8 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensities in the mouse dorsal skins were measured by an in vivo imaging system (IVIS Lumina XRMS Series III, PerkinElmer). The organs and tissues ...
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bioRxiv - Bioengineering 2023Quote: ... tumor engraftment in each mouse was quantified via bioluminescent imaging using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer) and Living Image Software ...
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bioRxiv - Cancer Biology 2023Quote: In ADM inhibition experiments wild type mouse organoids were imaged live after overnight acclimation using the Operetta High Throughput Screen imaging system (PerkinElmer) using 20× objective ...
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bioRxiv - Immunology 2023Quote: ... Tumor growth in live mouse was monitored every 5 days by bioluminescence imaging (BLI) using IVIS Spectrum In Vivo Imaging System (PerkinElmer) after 10mins intraperitoneally injection of 150mg/Kg D-luciferin ...
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bioRxiv - Cancer Biology 2021Quote: The intrinsic and extrinsic fluorescence emission spectra of the proteins were measured using an LS55 spectrofluorometer (Perkin Elmer, Waltham MA, USA). WT or N16D HsTPI (0.5 mg·mL-1 ...
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bioRxiv - Cell Biology 2020Quote: ... the PVDF membrane was washed with TBST and incubated with appropriate secondary antibodies (horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG), followed by enhanced chemiluminescence (ECL; Visual protein, Taipei, Taiwan) and the membrane was examined by ECL spectrophotometry (Perkin Elmer, Geliance 600 Camera Imaging system ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was transferred to a tube and the protein-bound [α-32P]dCTP was quantified in a Beckman Coulter LS 6500 Liquid Scintillation Counter using OptiPhase HiSafe 3 (PerkinElmer) as solvent.
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Microbiology 2020Quote: ... and adhesin were optimized by finding the hook point for the interaction by setting up a matrix of proteins in 96 well AlphaPlates (Perkin Elmer) in 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
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bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Biochemistry 2020Quote: ... with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol) from Perkin Elmer. Protein Assay (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
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bioRxiv - Physiology 2020Quote: ... glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) knock out mice were orally gavaged with 100 μCi of [9,10-3H(N)]-Triolein (Perkin Elmer, NET431001MC) suspended in olive oil ...
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bioRxiv - Immunology 2021Quote: ... The radioactive counts (cpms) of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer). Antibody index for each sample was calculated as follows ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... and hybridised with a probe against mouse Foxj1 according to the manufacturer’s instructions (ACD Systems #317091, #323110 and PerkinElmer #FP1487001KT, #FP1488001KT). Images were acquired using Andor Dragonfly spinning disk confocal microscope ...
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bioRxiv - Bioengineering 2021Quote: ... was drawn around the bottom half of each mouse with the total flux being calculated by Living Image® software (PerkinElmer; Total flux = the radiance (photons/sec ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin concentrations (from tail vein samplings) were determined using an ultrasensitive mouse insulin AlphaLisa detection kit (cat number AL204C) from Perkin Elmer.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Neuroscience 2022Quote: Fixed samples of Drosophila embryo nerve cords and dissociated mouse commissural neurons were imaged using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon 40X objective with Volocity imaging software ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-coupled anti-mouse-HRP was used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer). The signal was normalized to the relative cellular content as determined by immunoblotting of whole cell lysates probed with antibodies.
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...