Labshake search
Citations for PerkinElmer :
251 - 300 of 548 citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
-
bioRxiv - Cell Biology 2020Quote: ... washed twice with the same medium and pulse labeled for 15 min with 35S-Cys/Met (300 μCi, Cat. No. NEG072007MC, Perkin Elmer). After pulse ...
-
bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
-
bioRxiv - Microbiology 2022Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [α32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... probes were fluorescently labeled with Opal Dyes (Opal 520 product no. FP1487001KT, Opal 570 product no. FP1488001KT, Opal 690 product no. FP1497001KT; Perkin Elmer). Autofluorescence from lipofuscin was quenched using the reagent TrueBlack (Biotium ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
-
bioRxiv - Neuroscience 2024Quote: L5 PNs within 200 µm coronal slices of thy1-GFP-labeled dendrite arbors were imaged at 100x on an UltraVIEW VoX spinning disc confocal (Perkin Elmer) Nikon Ti-E-Eclipse microscope ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested after 48 hr transfection and nuclear extracts were prepared as previously described.78 The two strands of short probe was labeled with [γ-32P]-adenosine triphosphate (PerkinElmer). After that ...
-
bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
-
bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...
-
bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 μCi/ml and at given time points ...
-
bioRxiv - Cancer Biology 2020Quote: ... The reverse primer R3 (5′-GCGCCGGTAGTTGCCCTTCTC-3′) was labeled at the 5′ end using [γ-32P]-ATP (PerkinElmer Inc., Waltham, MA, USA) and T4 polynucleotide kinase (Takara) ...
-
bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
-
bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
-
bioRxiv - Biochemistry 2021Quote: ... the R2R and primer extension (PE) DNA primers (100 pmol) were labeled with [γ-32P]ATP (125 pmol; 6,000 Ci/mmol; 150 μCi/ μl; PerkinElmer Life Sciences) by incubating the DNA with T4 polynucleotide kinase (10 units ...
-
bioRxiv - Neuroscience 2021Quote: ... Biotin-labeled second-round aRNA was generated with an NTP-mix containing Biotin-11-CTP and Biotin-16-UTP (PerkinElmer, Boston, MA) at 2 mM ...
-
bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
-
bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
-
bioRxiv - Biophysics 2023Quote: ... stand and a 40 nt oligo-dT at the 5′ end was labeled at the 3′ terminus with [α-32P] dCTP (Perkin Elmer) and annealed to the single-stranded DNA M13mp18(24) ...
-
bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
-
bioRxiv - Developmental Biology 2020Quote: ... mouse dissociated commissural neurons and mouse dorsal spinal cord explants were imaged using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon 40X objective (for nerve cords and neurons ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... OsteoSense 680EX (80 nmol/kg/mouse, PerkinElmer, NEV10020EX). After 48 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
-
bioRxiv - Genetics 2019Quote: ... with the exception that the blue and green probes were labeled with coumarin-5-dUTP (custom synthesis, Perkin Elmer Life and Analytical Sciences) and fluorescein-12-dUTP (Perkin Elmer) ...
-
bioRxiv - Biochemistry 2019Quote: ... cells were stained with Draq5 and Rhodamine-labeled Annexin V and image acquisition was performed using the Opera QEHS confocal microscope (Perkin Elmer, Woodbridge, ON) with a 20x air objective ...
-
bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
-
bioRxiv - Biochemistry 2021Quote: ... alone or in the presence of 100 μM Na2S was assayed for its ability to autophosphorylate in a reaction containing 50 µCi of [γ-32P]-labeled ATP (6000 Ci/mmol, PerkinElmer Health Sciences), 100 mM Tris-HCl ...
-
bioRxiv - Physiology 2022Quote: ... The assay was a mobility shift assay based on charge differences before and after electrophoretic separation of product from fluorescently labeled substrate read using a Caliper EZ Reader (Perkin Elmer, Boston, MA). All reactions took place in the presence of excess nicotinamide adenine dinucleotide and the known SIRT1 inhibitor suramin was used as a control.
-
bioRxiv - Microbiology 2021Quote: ... SVC1-scramble and isogenic non-invasive SVC1 bacteria (lacking pSiVEC-scramble) were fluorescently labeled using the XenoLight RediJect 750 near-infrared fluorescent probe (Perkin Elmer, Waltham, MA). Nine-week-old female BALB/c mice (Jackson Laboratory ...
-
bioRxiv - Molecular Biology 2022Quote: U10 and CUGCUGUUUU (20 pmol) were chemically synthesized (Integrated DNA Technologies (IDT)) and 20 pmol RNA was radioactively labeled using [γ-32P]-ATP (PerkinElmer, 10mCi/ml) and 10 units T4 Polynucleotide Kinase (PNK ...
-
bioRxiv - Microbiology 2021Quote: ... and then living mouse and mouse organs were imaged and detected using the small animal in vivo imaging system (PerkinElmer, USA). The mouse organs were fixed with formalin ...
-
bioRxiv - Cell Biology 2019Quote: ... protein bands were visualized with enhanced chemiluminescent reagent (PerkinElmer) and exposed to X-ray films (Carestream ...
-
bioRxiv - Immunology 2021Quote: ... proteins were detected using Western Lightning Plus-ECL (PerkinElmer) and the iBright FL1000 Imaging System (Thermo Fisher ...
-
bioRxiv - Biochemistry 2021Quote: SPA PVT Protein A beads (500μl/sample, Perkin Elmer) were incubated for 2h with anti-GST antibodies (1:100) ...
-
bioRxiv - Biochemistry 2020Quote: ... and EasyTag EXPRESS [35S] Protein Labeling Mix (Perkin Elmer) for 30 min in a tissue culture incubator ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by enhanced chemiluminescence (PerkinElmer, cat# NEL105001EA). Figures show representative images of 3-5 biological replicates.
-
bioRxiv - Evolutionary Biology 2020Quote: ... gravity-flow protein purification with Lipidex 1000 (Perkin Elmer) chromatography was conducted ...
-
bioRxiv - Cell Biology 2021Quote: ... proteins were visualized with Western Lightning Plus substrate (PerkinElmer).
-
bioRxiv - Bioengineering 2023Quote: Proteins were directly radioiodinated with [125I]NaI (Perkin Elmer) using Pierce iodination reagent (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer) and Anti-FLAG Alpha Donor Beads (PerkinElmer ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were subsequently blotted onto PROTRAN Pure nitrocellulose membrane (PerkinElmer) using transfer buffer (25mM TRIS ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were detected with WesternLightning ECL Reagent (Perkin Elmer, Germany) and exposure of the membrane to CL-XPosure film (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were revealed using Lightning® Plus ECL (Perkin Elmer) and the bands were analysed by densitometry using ImageJ (NIH ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein signal was detected using enhanced chemiluminescence (ECL) reagent (PerkinElmer). The antibodies used were obtained from commercial sources ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... and 1 µL Express 35S protein labelling mix (Perkin Elmer) in the presence of 0.1% (w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... And proteins were visualized using enhanced chemiluminescence detection (NEL103E001EA, PerkinElmer) by AI600 (GE Healthcare).