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Citations for PerkinElmer :
101 - 150 of 186 citations for Recombinant Mouse CD8a Antigen Alpha Chain His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Bioengineering 2023Quote: ... Cardiomyocyte area and cardiomyocyte number were quantified by identifying cell membranes through staining tissue samples with fluorescent-tagged wheat germ agglutinin (WGA) and quantified using Mantra™ quantitative pathology workstation (PerKinElmer, Waltham, MA)17 ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse dissociated commissural neurons and mouse dorsal spinal cord explants were imaged using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon 40X objective (for nerve cords and neurons ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
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bioRxiv - Bioengineering 2022Quote: ... OsteoSense 680EX (80 nmol/kg/mouse, PerkinElmer, NEV10020EX). After 48 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
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bioRxiv - Microbiology 2021Quote: ... and then living mouse and mouse organs were imaged and detected using the small animal in vivo imaging system (PerkinElmer, USA). The mouse organs were fixed with formalin ...
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bioRxiv - Bioengineering 2022Quote: ... plates were incubated with anti-mouse IgG-Europium antibody (PerkinElmer) for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... and HRP-labeled goat anti-mouse (PerkinElmer Life Science, USA) staining ...
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bioRxiv - Genetics 2023Quote: ... each mouse was gavaged with 1 µCi 59FeCl2 (Perkin Elmer) in 2.21 mM FeCl3 in 1 M ascorbic acid filter-sterilized ...
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bioRxiv - Bioengineering 2020Quote: ... Whole-mouse luminescence data were analyzed using Living Image software (PerkinElmer). Figures were designed in Adobe Illustrator.
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bioRxiv - Biochemistry 2024Quote: ... HRP labelled goat anti-mouse IgG (Perkin Elmer: NEF822001EA, 1:10,000), HRP labelled goat anti-rabbit IgG (Perkin Elmer ...
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bioRxiv - Pathology 2022Quote: ... or Immpress anti-mouse/rabbit coupled with tyramide amplification kit (PerkinElmer, Inc). Nuclei were counterstained with DAPI.
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bioRxiv - Cell Biology 2022Quote: ... 1:200 dilution of anti-mouse IgG donor bead (Perkin Elmer, AS104D) and a 1:200 dilution of anti-rabbit IgG acceptor bead (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... Each mouse received 2 nmol of MMPSense 680 (150 μl; Perkin Elmer, France) or Cat K 680 FAST™ (100 μl ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit, anti-mouse [PerkinElmer]) and HRP-reactive OPAL fluorescent reagents (PerkinElmer) ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were 1:10,000 of HRP-labeled α-mouse (for Flag) (PerkinElmer) or α-rabbit (PerkinElmer ...
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bioRxiv - Cancer Biology 2023Quote: ... The appearance of mouse metastasis was monitored by IVIS (IVIS Lumina III, Perkin Elmer) after injecting the animals with luciferine (150 mg/kg ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 min with horseradish peroxidase-conjugated secondary polymer (anti-mouse/anti-rabbit, Perkin Elmer), and 10 min with horseradish peroxidase-reactive OPAL fluorescent reagents (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 minutes after intraperitoneal injection of Potassium D-Luciferin solution (Perkin Elmer, 3 mg/mouse). Luminescence values for all xenografts were quantified as the average radiance (photons/s/cm2/sr ...
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bioRxiv - Cancer Biology 2022Quote: ... each mouse was given an intra-peritoneal (i.p.) injection of luciferin (PerkinElmer, Part Number #122799) at a dose of 150mg/kg body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... an anti-mouse IgG (1.5nM) labelled with fluorescence donor Eu-W1024 (Perkin Elmer product AD0076) and an anti-rabbit IgG (14.3nM labelled with acceptor (APC) ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit polyclonal and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Microbiology 2019Quote: ... USA) Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Physiology 2022Quote: An oral load of D-[U-14C]-glucose (5μCi/mouse, Perkin Elmer, Boston, MA, USA) in 1.39M of glucose-PBS solution was given to adult mice by gavage after 4h fasting ...
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bioRxiv - Cancer Biology 2022Quote: ... The lung bioluminescence intensity signal of every mouse was determined using Living Image software (PerkinElmer). Tamoxifen treatment (intraperitoneally ...
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bioRxiv - Cancer Biology 2023Quote: ... was injected intraperitoneally in each mouse and mice were imaged by IVIS imaging system (PerkinElmer) after 10 mins ...
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bioRxiv - Immunology 2021Quote: Levels of cytokines in mouse plasma and exudates were investigated using ProcartaPlex immunoassays (Perkin Elmer, USA) with a custom panel of 21 cytokines according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... with a PBS solution containing 3mg of luciferin/mouse (XenoLight D-Luciferin - K+ Salt, PerkinElmer, cat. 122799) and imaged using an In Vivo Imaging system (IVIS ...
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bioRxiv - Immunology 2020Quote: ... with a PBS solution containing 3mg of luciferin/mouse (XenoLight D-Luciferin - K+ Salt, PerkinElmer, cat. 122799) and imaged using an IVIS Spectrum In Vivo Imaging system (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse recPrP23-231 at 65 nM in PBS was coated to 384 well HB plates (Perkin Elmer), overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...