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Citations for PerkinElmer :
1 - 50 of 270 citations for Recombinant Influenza A virus HA protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... HA-binding protein staining was achieved with 1/100 strepavidin HRP (Perkin Elmer, Massachusetts, USA) solution for 30 min at room temperature and 3,3’-diaminobenzidine (Liquid DAB + substrate Chromagen System ...
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bioRxiv - Microbiology 2021Quote: The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
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bioRxiv - Genetics 2021Quote: ... Grain protein content (%) and grain yield (t/ha) was collected using a Perten DA 700 NIR analyzer (Perkin Elmer, Sweden) and Wintersteiger Nursery Master combine (Ried im Innkreis ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Microbiology 2020Quote: ... All virus supernatant was quantified via p24 ELISA (Perkin Elmer).
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bioRxiv - Immunology 2020Quote: ... Serum-virus mixtures were then transferred to 96-well plates (PerkinElmer), and 1.0×104 MDCK-SIAT1-PB1 cells51,92 were added into each well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... pseudotyped virus treated hESC-derived cardiomyocytes in CellCarrier-96 Ultra Plates (PerkinElmer) were washed 3x with 100 μL PBS before complete submersion in 2-4% formaldehyde solution for 30 mins ...
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) and by real time PCR ...
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bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) or by qPCR ...
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bioRxiv - Microbiology 2019Quote: ... WSN-GFP virus expression was detected by the Operetta-High Content Imaging System (PerkinElmer Inc.), and the percentage of GFP-positive cells were analyzed by Harmony4.1 software (PerkinElmer Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
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bioRxiv - Immunology 2020Quote: ... The virus infectivity titers were determined by measuring the luciferase activity using Britelite luciferase substrate (PerkinElmer Inc.) in a luminometer (Victor X2 ...
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bioRxiv - Microbiology 2022Quote: ... the virus was identified using liquid scintillation analyzer TRI-carb 2910 TR (Perkin Elmer, Waltham, Massachusetts, US). The virus containing fractions were collected and pelleted as earlier ...
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bioRxiv - Immunology 2021Quote: ... The luciferase signal was determined in live animals one day after virus injection and 10 minutes after intraperitoneal injection of 150 mg/kg D-luciferin (PerkinElmer) using the in vivo imaging system NightOWL II LB 983 (Berthold ...
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bioRxiv - Neuroscience 2021Quote: ... Image analysis scripts for HA+/NeuN+ cells were developed using Acapella® Studio 5.1 (PerkinElmer Inc.) and the integrated Acapella® batch analysis as part of the Columbus® system.
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The library preparation has been done in combination with benchtop liquid handlers: Zephyr G3 NGS Workstation (PerkinElmer) and Mantis (Formulatrix) ...
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2, PerkinElmer Life Sciences).
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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bioRxiv - Cell Biology 2021Quote: Live cell labeling of hydroxyapatite (HA) was performed with 1 μM OsteoSense 680EX Fluorescent Imaging Agent (Perkin Elmer) in BRDM for 20 minutes at 37 °C.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... cryogels were administered into the dorsal flank of an anesthetized mouse and the fluorescent intensity of the Cy5-HA cryogel was quantified using an IVIS spectrometer (PerkinElmer) at predetermined timepoints and analyzed using LivingImage software (PerkinElmer) ...
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bioRxiv - Cell Biology 2019Quote: A centrifuge-free RABiT-II system was used for automated sample processing after culturing: The system consists of two part: the first (Figure 1) has an automated liquid-handling system (JANUS, PerkinElmer) with integrated automated microplate handler (Twister3 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 μCi/ml and at given time points ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Cell Biology 2019Quote: ... protein bands were visualized with enhanced chemiluminescent reagent (PerkinElmer) and exposed to X-ray films (Carestream ...
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bioRxiv - Immunology 2021Quote: ... proteins were detected using Western Lightning Plus-ECL (PerkinElmer) and the iBright FL1000 Imaging System (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: SPA PVT Protein A beads (500μl/sample, Perkin Elmer) were incubated for 2h with anti-GST antibodies (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... and EasyTag EXPRESS [35S] Protein Labeling Mix (Perkin Elmer) for 30 min in a tissue culture incubator ...