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Citations for PerkinElmer :
201 - 250 of 544 citations for Recombinant Human TNFRSF17 protein Fc His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with RNA probes in vitro transcribed with 32P-labeled UTP (PerkinElmer Life Sciences). Radioactive signals were detected with a phosphorimager and signals were quantified with Quantity ONE software (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... The DIG-labeled probes were amplified with horseradish peroxidase using Cy3.5 tyramide signal amplification kit (PerkinElmer) for 5 to 10 min.
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bioRxiv - Developmental Biology 2023Quote: ... The annealed synthetic oligonucleotide DNA was labeled with [α-32P]dCTP (Perkin Elmer NEG013H250UC, 3000Ci/mmol) by Klenow polymerase ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Molecular Biology 2021Quote: ... metabolically labeled for 20 min with 35S-Met (50 μCi 35S-Met, 1175 Ci/mmol, Perkin Elmer) and then lysed in SDS-lysis buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer, BLU502A250UC) using 20U T4 polynucleotide kinase (NEB ...
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bioRxiv - Cancer Biology 2020Quote: Imaging of fluorescently labeled tissue sections was performed utilizing Nuance-FX multispectral imaging system (Caliper LifeSciences, PerkinElmer), using full capabilities of its Tunable Liquid Crystal imaging module ...
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bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 10mM sodium butyrate and 0.25 µCi/µl (4.9 Ci/mmol) of [3H]-labeled Acetyl-CoA (Perkin Elmer) or 0.15mM unlabeled Acetyl-CoA (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: DNA probes (U1-SH, U4-14B, and U6-SH) were end labeled with [γ-32P]-ATP (PerkinElmer) using T4 PNK (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Riboprobes were labeled with either digoxigenin-UTP or dinitrophenyl-11-UTP (DNP; Perkin Elmer, Waltham, MA, USA). A DNP-labeled probe and a DIG-labeled probe were hybridized simultaneously ...
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bioRxiv - Microbiology 2019Quote: ... while the 14C-labeled methyladenosines were detected with a Packard radiomatic 515TR flow scintillation analyzer (Perkin Elmer).
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bioRxiv - Microbiology 2019Quote: ... was prepared and labeled according to manufacturer’s guidelines using [gamma- 32P]-ATP (6,000 Ci/mmol) (Perkin Elmer) and T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Biophysics 2021Quote: ... The primer was labeled on its 5’end with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol−1) and T4 polynucleotide kinase (Thermofisher) ...
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Tris buffer containing trace amount of 32P-labeled orthophosphoric acid (∼1.2 pmol and ∼1 µCi, PerkinElmer) and different concentrations of cold phosphate (NaH2PO4 ...
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bioRxiv - Genetics 2020Quote: ... DNA methylation was measured by the incorporation of tritiated methyl groups from radioactively labeled SAM (Perkin Elmer) into the biotinylated substrate using an avidin–biotin methylation plate assay47 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer), with U6 served as loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then placed in a tracer buffer containing 1-125-labeled OVTA ligand (Perkin Elmer NEX2540) for 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were incubated for 5 min before addition of 200μCi 35S-labeled methionine/cysteine mixture (PerkinElmer) and incubation for 1 hr at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 0.5 uCi of 56 mCi/mmol [14C] acetic acid (Perkin Elmer Cat# NEC084H001MC) per well overnight ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Neuroscience 2020Quote: Tiled images were acquired using Olympus VS120 Virtual Slide Microscope or PE Ultraview spinning disk confocal microscope (PerkinElmer), which were stitched together using the Volocity software (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... and imaged onto the active area of two fibre-coupled avalanche photodiode detectors (APDs; Perkin Elmer, SPCM-AQRH-15-FC) using a cubic non-polarizing beam-splitter (Linos ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... Alpha-LISA kits specific for human Aβ1–X (AL288C, PerkinElmer) and Aβ1–42 (AL276C ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown until OD600 ∼ 0.02 and labeled with 50 μCi/ml 32P-orthophosphate (900 mCi/mmol; PerkinElmer). When OD600 reached 0.2-0.4 ...
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bioRxiv - Microbiology 2020Quote: ... was used for hybridization with oligonucleotides 5’-end labeled with 6,000 Ci/mmol γ-32P ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled using an 35S Met/Cys labeling mixture at 200 µCi/ml (EasyTag Express35S; Perkin Elmer) for 30 minutes in media that contained drug treatments and that was 20 percent MEF culture medium as above and 80 percent DMEM lacking Met/Cys and with dialyzed FBS ...
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bioRxiv - Biophysics 2019Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Bioengineering 2020Quote: MTs were transferred with a tip quickly coated in fetal calf serum (FCS) into a CellCarrier-384 Ultra Microplates (6057300, Perkin Elmer). They were then fixed in 4% PFA (P6148 ...
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bioRxiv - Microbiology 2023Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Biophysics 2024Quote: Single-point FCS data were acquired with an Insight Cell confocal microscope (Evotec Technologies, Hamburg, Germany, now PerkinElmer, Waltham, MA, USA). Excitation was performed using a 488 nm continuous wave solid state diode-pumped laser (Sapphire 488-20/460-10 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Biochemistry 2019Quote: ... Oligonucleotides were 5’-end-labeled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1; Perkin Elmer) using polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... primer (5’-AGUUGUUGAUC) was 32P-labeled at the 5′ position upon incubation with [γ-32P] ATP (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with canine rough microsomes (tRNA probes, College Station, TX) and EasyTag 35 S-labeled methionine (PerkinElmer, Waltham, MA) for one hour at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were labeled with 250-1000 µCi/ml of [35S]-Express label (1100 Ci/mmol; PerkinElmer, Boston, MA) at 37°C depending on the length of the pulse ...
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bioRxiv - Immunology 2021Quote: ... The 3H-labeled products were isolated using DEAE-Sephadex A-50 columns and quantitated by liquid scintillation counting (PerkinElmer). Non-isotopic methylated RNA fragments were prepared using cold SAM (Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...