Labshake search
Citations for PerkinElmer :
601 - 650 of 1906 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: Cells were plated on black 384-well CellCarrierUltra (PerkinElmer) plates one day before adding inhibitors at a density of 2.5×104 cells/ml in 20 μl of media/well ...
-
bioRxiv - Cell Biology 2022Quote: Cells were plated on black 384-well CellCarrierUltra (PerkinElmer) plates one day before adding inhibitors at a density of 1.25×104 cells/ml in 20 μl of media/well ...
-
bioRxiv - Microbiology 2022Quote: ... cells were lysed with Britelite Plus reagent (Perkin Elmer), and luciferase activity was measured as relative luminesce units (RLUs) ...
-
bioRxiv - Neuroscience 2022Quote: ... 96-multiwell cell-carrier plates were used (PerkinElmer, USA). Four days after plating ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were harvested with 20 μL Britelite Plus (PerkinElmer), and luminescence was measured on a BioTek Synergy Neo multimode plate reader ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cells’ luciferase activity (Promega, #E1483 or PerkinElmer, #6016716) had been confirmed before injection ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were imaged using the IVIS Spectrum (Perkin Elmer) and Living Image software (version 4.3.1 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were analyzed by the Harmony software (Perkin Elmer) using a 63X water immersion objective lens after washing by PBST buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured by ATPlite Luminescence Assay (PerkinElmer), and IC50 was calculated using GraphPad Prism 7 ...
-
bioRxiv - Immunology 2023Quote: ... the cells were then lysed using BriteLite (Perkin Elmer) luciferase readout reagent ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were collected and lysed with luciferase substrate (PerkinElmer), then underwent luminescence intensity measurement by BioTek microplate reader ...
-
bioRxiv - Immunology 2024Quote: ... cells were lysed with Britelite Plus reagent (Perkin Elmer), and luciferase activity was measured as relative luminesce units (RLUs) ...
-
bioRxiv - Microbiology 2019Quote: ... 25µL of the warmed suspension was mixed with 150µL of MOPS-CDM containing 150µCi mL-1 of H3[32P]O4 (2mCi mL-1 in Water; PerkinElmer, Waltham, MA) for 30 minutes ...
-
Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... TSA Plus fluorophores fluorescein Cyanine 3 (1:1500 dilution) and Cyanine 5 (1:3000 dilution) (Perkin Elmer, Waltham, MA, USA) were used for signal detection ...
-
bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
-
bioRxiv - Genetics 2023Quote: ... A sample of cells were stained with 10 µg ml–1 Hoechst plus 1:500 acridine orange/propidium iodide solution and counted on an Opera Phenix confocal microscope (PerkinElmer). A total of 100,000 cells per well were seeded into neural coating medium-filled 96-well plates in 100 µl per well of neural medium comprising Brainphys medium with 1× B27 plus vitamin A ...
-
bioRxiv - Immunology 2023Quote: N/TERT cells or human primary keratinocytes of various genotypes were seeded at a cell density of 10,000 cells/well in a black 96-well plate (PerkinElmer, CellCarrier-96 Ultra, #6055300) or a cell density of 80,000 cells/well in black 24-well plates (Cellvis ...
-
bioRxiv - Cell Biology 2023Quote: ... the cell plates were immediately started for live cell imaging on an automated spinning-disk confocal high-content imaging system (Perkin Elmer, Opera Phenix) using a 40X water immersion objective lens under 5% CO2 and 37 °C condition ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were seeded at 25000 cells per well in culture medium into white bottom opaque 96 well plates (Perkin Elmer cat no. 5680). Plates were incubated at 37 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... anti-DNP-HRP antibody (1:1000, Perkin Elmer) and tyramide signal amplification (Perkin Elmer) ...
-
bioRxiv - Developmental Biology 2020Quote: ... A buffer containing Cy3 (PerkinElmer, Waltham, 1:50) was used to stain the samples for 40 min.
-
bioRxiv - Microbiology 2021Quote: ... and 1 U of AmpliTaq polymerase (Perkin Elmer). The mixture was subjected to 40 cycles of amplification using a DNA thermal cycler 2720 (Perkin Elmer) ...
-
bioRxiv - Neuroscience 2020Quote: ... TSA® Plus Cyanine 5 (PerkinElmer, 1:1,000) was used as a secondary fluorophore for C1 probes (Gtpbp2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... incubated for 1 min ECL (Perkin Elmer, USA) and the signal was determined with an INTAS ECL Chemocam imager (INTAS Science Imaging ...
-
bioRxiv - Biophysics 2022Quote: ... and 1:125 Streptavidin-XL665 (HTRF acceptor, PerkinElmer) in assay buffer (25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
-
bioRxiv - Microbiology 2019Quote: ... Sodium Salt (Perkin Elmer, NEC084H001MC, 1 mCi/mL). For INH controls ...
-
bioRxiv - Cell Biology 2021Quote: ... TSA® Plus Cyanine 3 (PerkinElmer, 1:1,500) was used as a secondary fluorophore for Hbs1l-C2 probes.
-
bioRxiv - Developmental Biology 2022Quote: ... and anti-DNP-HRP (PerkinElmer FP1129; 1:100) were used in combination with DyLight488-tyramide and DyLight680-tyramide ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 μM [3H] SAM (82.3 Ci/mmol, PerkinElmer) and various amounts of refolded RNA in 1X binding buffer ...
-
bioRxiv - Developmental Biology 2022Quote: ... and Opal 690 (1:1500, PerkinElmer, Cat. FP1497001KT).
-
bioRxiv - Neuroscience 2024Quote: ... A distinct Opal fluorophore (Perkin Elmer, 1:500) was assigned to each probe and sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole ...
-
bioRxiv - Cell Biology 2020Quote: ... mice were anesthetized with 2% isoflurane and renilla bioluminescence was visualized/quantified by the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
-
bioRxiv - Cell Biology 2020Quote: ... the mice were anesthetized with 2% isoflurane and firefly bioluminescence was visualized/quantified using the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
-
bioRxiv - Plant Biology 2020Quote: ... 5 to 10 mg powder were mixed with 2 ml scintillation cocktail and counts per minute (cpm) recorded with a TRI-Carb 2810TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA).
-
bioRxiv - Biochemistry 2021Quote: ... Plates were incubated at room temperature for 2 h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences). All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100 μM forskolin in each cell line ...
-
bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
-
bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
-
bioRxiv - Cancer Biology 2023Quote: ... mRNA was then extracted from 2 ug of total RNA per sample using NEXTFLEX® Poly(A) Beads 2.0 (Perkin Elmer, Austin, Texas, USA). Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer ...
-
bioRxiv - Microbiology 2019Quote: ... 10% (v/v) glycerol) supplemented with 200 μCi ml−1 (65 nM) of [γ-33P]ATP (PerkinElmer, 3000 Ci mmol−1) for 10 minutes at room temperature in order to obtain the optimal FrzECheA autophosphorylation activity ...
-
bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
-
bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
-
bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed with TNT followed by a 1 h incubation with streptavidin-conjugated horseradish peroxidase (1:1000; PerkinElmer; #NEL750001EA) in TNB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... The probes were visualized using Opal 520 and Opal 570 reagent (1:500 and 1:1000, respectively; PerkinElmer, Woodbridge, ON, CA). PHOX2B immunoreactivity was detected using mouse monoclonal PHOX2B antibody (B- 11 ...
-
bioRxiv - Immunology 2022Quote: N-T TERT cells of various genotypes were seeded at a cell density of 10000 cells/well in a black 96 well plate (PerkinElmer, CellCarrier-96 Ultra, #6055300). The next day cells were treated with chemicals and stained with 1μg/ml dilution of propidium iodide (PI ...
-
bioRxiv - Cell Biology 2020Quote: Live-cell imaging of LN229 cells expressing MyrPalm-mCherry was performed using the Opera Phenix spinning disk confocal microscope (Perkin Elmer, Waltham, MA, USA). LN229 cells were seeded on SensoPlate 96-well glass bottom plates from Greiner Bio-One (655892) ...
-
bioRxiv - Microbiology 2023Quote: ... Compounds were incubated for 48 h and cell viability was determined by measuring the adenosine triphosphate (ATP) content of the cells using the ATPlite kit (PerkinElmer, Waltham, MA, Cat: 6016941) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: Cells were seeded in CellCarrier Ultra-384 well dishes (PerkinElmer) at a density of 5.5 x104 cells/mL (50 µL/well) ...