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Citations for PerkinElmer :
51 - 100 of 127 citations for Recombinant Human Integrin Alpha 8 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Phosphorylation was determined by alpha-screen bead-based technology using the Amplified Luminiscent Proximity Homogeneous Assay kit (PerkinElmer, Waltham, MA, USA) and the Enspire Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were initiated by the addition of GTP to a final concentration of 40 uM containing a trace amount of [alpha-32P]-GTP (Perkin Elmer) and incubated for 20 min at 30°C ...
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bioRxiv - Physiology 2020Quote: ... 1,3-[3H]-dipropyl-8-cyclopentylxanthine ([3H]-DPCPX) was purchased from PerkinElmer (Life and Analytical Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Cancer Biology 2019Quote: ... AlphaLISA assays were performed in 96-well half area white plates (6005560) and read in an EnSpire Alpha 2300 Mutilabel Plate reader (Perkin Elmer, Inc.). A 96-well white plate was filled with 5 µl of EVs or 10 µl of CM ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Neuroscience 2019Quote: ... The Fluo-8 signal was measured using the FLIPR TETRA system (PerkinElmer).
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bioRxiv - Cancer Biology 2023Quote: ... dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer) confocal screening microscope ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Immunology 2022Quote: ... 8 min after injecting subcutaneously a luciferin substrate solution (15mg/ml, Perkin Elmer) at 10μl/g of body weight.
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bioRxiv - Molecular Biology 2019Quote: ... One-milliliter cultures were labeled with 50 µCi [8-3H] adenine (NET06300 PerkinElmer) for 2 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 8-10 mins were allowed before image acquisition using the IVIS (Perkin Elmer). Analysis of luminescence was done using the Living Image programme (Perkin Elmer) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... washed and finally incubated for 8 min at RT with Tyramide-Cy5 (Perkin Elmer) dil ...
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bioRxiv - Cancer Biology 2022Quote: ... were dispensed per well 8 h prior to imaging at Opera Phenix (Perkin Elmer) confocal screening microscope ...
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bioRxiv - Biophysics 2021Quote: ... the cells were treated by a constant concentration of 125I-CCK-8 (40 pM, PerkinElmer) plus 8 different doses of CCK-8 (1 pM to 10 μM ...
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bioRxiv - Biophysics 2021Quote: ... the cells were treated by a constant concentration of 125I-CCK-8 (30 pM, PerkinElmer) plus 8 different concentrations of gastrin-17 (128 pM–10 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Supernatant were used for analysis through GC-MS (PerkinElmer Mass Spectrometer Clarus SQ 8 C). The calibration curve was made using Synthetic IAA (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... Between 3 and 8 fields per well were acquired and analyzed using Acapella 2.6 (PerkinElmer). For all nuclear markers ...
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bioRxiv - Microbiology 2022Quote: ... 8×103 naïve U2OS cells/well were plated on viewPlate-96black (Perkin Elmer, Waltham, USA). Cells were spinoculated in the presence of 8 μg/mL of polybrene with serial dilution of precleared supernatant from infected LEC ...
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bioRxiv - Biochemistry 2023Quote: ... followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf; PerkinElmer Inc., Waltham, USA) kept at 60 °C ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Immunology 2021Quote: ... with the last 8 hours in the presence of [3H] thymidine (1 mCi/well, NET027001MC; PerkinElmer). Incorporated [3H] thymidine was counted by a BetaPlate scintillation counter (Wallac ...
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bioRxiv - Neuroscience 2020Quote: The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal detection was performed by 8 min incubation with the PerkinElmer TSA Plus Cyanine 3 kit (PerkinElmer, NEL744E001KT ...
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bioRxiv - Cell Biology 2021Quote: Primary human hepatocytes were seeded in 96-well black CellCarrier plates (PerkinElmer) at a density of 5×103 cells per well ...
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bioRxiv - Immunology 2021Quote: Human fibroblasts cells were seeded on CellCarrier-96well Ultra microplate (Perkin Elmer) and incubated for at least 24 hours in growth media ...
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bioRxiv - Biochemistry 2023Quote: 5x104 human neutrophils were seeded in a black 96-well plate (PerkinElmer) in HyClone HBSS +Ca ...
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bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... the mice were subjected to inhalational anesthesia using an XGI-8 Gas Anesthesia System (PerkinElmer, Waltham, MA, USA) containing a mixture of 4% isoflurane (ISOTROY 100 ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT and signal amplification for 8 min using Cy3 (NEL704A001KT, Perkin Elmer) 1:50 in amplification diluent ...
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bioRxiv - Cell Biology 2021Quote: The human glioblastoma U87 MG-Red-FLuc (Bioware Brite, PerkinElmer, Waltham, Massachusetts, USA) was the authorized cell line in all experiments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Multispectral microscopy of the human biopsies was performed using a Vectra 3.0 (PerkinElmer) equipped with 20x and 40x objectives ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then separated on a HayeSep column (7’ HayeSep N 1/8’’ Sf; PerkinElmer Inc., Waltham, USA), followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf ...
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bioRxiv - Immunology 2020Quote: ... Bound IgG was detected with Eu-N1 Anti-Human IgG (Perkin Elmer, 1244-330). using DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Genomics 2021Quote: Human primary AMSCs and PHH were plated in 96-well CellCarrier plates (Perkinelmer #6005550). AMSCs were differentiated for 14 days and high content imaging was performed at day 0 ...
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bioRxiv - Cell Biology 2021Quote: Human Fibroblasts Cells were seeded on CellCarrier-384 or 96well Ultra microplate (Perkin Elmer) and incubated for at least 24 h in growth media ...
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bioRxiv - Microbiology 2022Quote: ... was phosphorylated in vitro with His-MBP-PknBMtb at 0.015 μg/μl for 30 minutes at room temperature with 0.15μCi/μl ATP [γ-32P] (5×10-8 μmol) (3000Ci/mmol, 10mCi/ml, PerkinElmer BLU002A250UC) and 2mM MnCl2 in buffer (50mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Uptake was measured at room temperature by mixing 100 µl of cells with 100 µl of transport buffer containing 8 µM of [14C] glycerol (0.125 µCi each in triplicates, PerkinElmer Inc.). Incorporation of radioactivity was determined as described above.
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bioRxiv - Neuroscience 2024Quote: ... 100 µL of cell homogenate (0.05 mg protein/well) and 100 µL of [3H]8-OH-DPAT (0.5 nM final concentration, 170 Ci/mmol, Perkin Elmer) in a final volume of 1 mL ...
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bioRxiv - Cancer Biology 2024Quote: ... was calculated for each mouse using a circular region of interest with the mouse in a prone position and normalized to the value obtained at the same area before starting treatment administrations (day 8) using the IVIS® Spectrum Living Image® 4.3.1 software (PerkinElmer). Tumor growth was also monitored thrice a week from day 15 by measuring tumor volume with a Vernier caliper using the modified ellipsoid volume formula ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Neuroscience 2020Quote: ... mammalian CHO K1 cells stably overexpressing apo-aequorin and human Gα16 (ES-000-A24, PerkinElmer) were transiently transfected with Ce-npr-4a/pcDNA3.1 ...