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Citations for PerkinElmer :
1 - 50 of 331 citations for Recombinant Human IL6 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... biotinylated cGMP (PerkinElmer biotinylated cGMP ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Genomics 2019Quote: ... FISH experiments were performed using human fosmids containing non-duplicated region directly labeled by nick-translation with Cy3-dUTP (PerkinElmer), Cy5-dUTP (PerkinElmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Biotinylated cGMP was bought from PerkinElmer and BIOLOG ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and biotinylated-His6 substrate (PerkinElmer, cat # 6760303M) were used according to manufacturer instructions with minor modifications ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Biochemistry 2023Quote: Endogenously tagged HiBiT cells were plated in 96-well plates (PerkinElmer) at a density of 0.5×106 cells/mL ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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bioRxiv - Microbiology 2021Quote: ... an equal volume of biotinylated SRC1 peptide/Europium-labeled streptavidin (Perkin Elmer) was added to biotynlated RORγ/APC-labeled strepavidin (Perkin Elmer) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA probes were tagged with dinitrophenol-11-UTP (DNP, Perkin Elmer, Waltham, Massachusetts), fluorescein-12-UTP (FITC ...
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bioRxiv - Cancer Biology 2020Quote: H2B-mRFP tagged cells were imaged using an OperaTM High Content Screening instrument (PerkinElmer), equipped with a 20x air objective (LUCPLFLN ...
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bioRxiv - Bioengineering 2024Quote: ... A non-invasive IVIS Spectrum Imaging System (Perkin Elmer, Waltham, MA) was used to record the total amount and distribution of dye molecules delivered to the cisterna magna based on their photon flux ...
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bioRxiv - Neuroscience 2020Quote: ... avidin-biotinylated horseradish peroxidase kit (Vector) followed by fluorescein tyramide signal amplification (Perkin Elmer) were applied per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with biotinylated tyramide (Perkin Elmer; Opal 4-color Fluorescent IHC kit) diluted at 1:50 in amplification buffer for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... Non-specific antibody binding was avoided using Blocking Reagent™ (Perkin Elmer) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear RELA was measured in all non-border cells using Harmony software (PerkinElmer). In R ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were tagged with GFP/luciferase to facilitate tracking via In Vivo Imaging Software (IVIS, Perkin Elmer)51 ...
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bioRxiv - Biochemistry 2023Quote: ... and biotinylated-JQ1 at 10 nM was added in 10 μL to 384-well plates (AlphaPlate-384, PerkinElmer). Plates were spun down at 1000 rpm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and biotinylated-JQ1 at 20nM was added in 10 µL to 384-well plates (AlphaPlate-384, PerkinElmer, USA). Plates were spun down at 150x g ...
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bioRxiv - Neuroscience 2021Quote: ... U251 MG (KO) cells were plated on non-coated CellCarrier 96-well plates (PerkinElmer; #6005550) or 6-well plates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... We replaced non-radiolabeled GTP with 750 μCi/mL 32P-α-GTP (3000 mCi / mmol; PerkinElmer) for production of [5’-α-32P]-pGpp ...
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bioRxiv - Microbiology 2020Quote: ... We replaced non-radiolabeled ATP with 750 μCi/mL 32P-γ-ATP (3000 mCi/mmol; PerkinElmer) for production of [3’-β-32P]-ppGpp ...
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bioRxiv - Microbiology 2020Quote: ... We replaced non-radiolabeled GTP with 750 μCi/mL 32P-α-GTP (3000 mCi / mmol; PerkinElmer) for production of [5’-α-32P]-(p)ppGpp ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Biochemistry 2023Quote: ... to a fixed concentration of target (e.g., biotinylated PTM-defined nucleosome) with the appropriate Alpha Donor and Acceptor beads (Perkin Elmer) (Jain et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... and 10 μL of 12 nM biotinylated nucleosome (or its mutants) were mixed and incubated in OptiPlate-384 microplate (PerkinElmer) for 60 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor growth monitoring was performed non-invasively twice a week by injecting Xenolight D-Luciferin (Perkin Elmer) and recording relative light units (r.l.u. ...
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bioRxiv - Cell Biology 2024Quote: ... high-content non-confocal imaging was performed using the Opera Phenix High-Content Screening System (Perkin Elmer) with a 20× water immersion lens and 568-nm lasers ...
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... bound cytokines were detected by biotinylated anti-IFN-γ (clone 4s.B3) and quantified using a Victor2 D time-resolved fluorometer (Perkin Elmer). The % activity was calculated by dividing the SI of mutated peptides by the SI of the wild-type peptide ...
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Molecular Biology 2023Quote: ... The non-volatile products were measured in a Tri-Carb 2810 TR Liquid Scintillation Counter (PerkinElmer; Waltham, MA) after 48 h ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cardiomyocyte area and cardiomyocyte number were quantified by identifying cell membranes through staining tissue samples with fluorescent-tagged wheat germ agglutinin (WGA) and quantified using Mantra™ quantitative pathology workstation (PerKinElmer, Waltham, MA)17 ...
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bioRxiv - Developmental Biology 2019Quote: ... Per well nine non-overlapping images were acquired and analyzed with the Operetta High Content Screening System microscope (PerkinElmer) and Harmony 4.1 software.