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Citations for PerkinElmer :
1 - 50 of 353 citations for Recombinant Human Fms related Tyrosine Kinase 3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Biochemistry 2021Quote: ... L-[3H]tyrosine (10 Ci/mol; PerkinElmer) and L-[3H]alanine (5 Ci/mol ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Cell Biology 2021Quote: AlphaLISA® SureFire® Ultra™ cellular kinase immunoassays were used to measure endogenous kinase activity of p-mTOR (Ser2448) (PerkinElmer, #ALSU-PMTOR-C500), p-ERK 1/2 (Thr202/Tyr204 ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Biochemistry 2023Quote: Endogenously tagged HiBiT cells were plated in 96-well plates (PerkinElmer) at a density of 0.5×106 cells/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a given histidine kinase was first autophosphorylated with [γ-32P]-ATP (Perkin Elmer) for 90 minutes at 30 °C to drive autophosphorylation and then mixed at a 1:4 molar ratio with a response regulator (1 μM EnvZ ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were radiolabelled with 32P-ATP by poly nucleotide kinase (PNK) (PerkinElmer PNK) for 1 hour at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Neuroscience 2021Quote: ... RNA probes were tagged with dinitrophenol-11-UTP (DNP, Perkin Elmer, Waltham, Massachusetts), fluorescein-12-UTP (FITC ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Cancer Biology 2020Quote: H2B-mRFP tagged cells were imaged using an OperaTM High Content Screening instrument (PerkinElmer), equipped with a 20x air objective (LUCPLFLN ...
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bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Molecular Biology 2020Quote: mTOR kinase activity was monitored by LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET, PerkinElmer) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Developmental Biology 2019Quote: ... and cyanine 3 (PerkinElmer), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... cyanine 3 (PerkinElmer #NEL744E001KT), and cyanine 5 (PerkinElmer #NEL745E001KT) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were tagged with GFP/luciferase to facilitate tracking via In Vivo Imaging Software (IVIS, Perkin Elmer)51 ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Biochemistry 2020Quote: ... 50 µg/mL PEG20,000 (SERVA; Cat. # 33138), ATP (Sigma; Cat. # A-7699) (variable, corresponding to ATP-Km of the respective kinase, [33P]- γ-ATP (PerkinElmer; Cat. # NEG302H): approx ...
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SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomesbioRxiv - Biochemistry 2022Quote: ... and lower phase (containing [3-3H]-sphingosine, [3-3H]-ceramides, [3-3H]-SM) were quantified by a scintillation counter (Perkin Elmer Tri-Carb Liquid Scintillation Analyzer).
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...