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Citations for PerkinElmer :
151 - 200 of 307 citations for Recombinant Human EIF4EBP1 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... To metabolically label the cells 5uL of EasyTag EXPRESS 35S protein labeling mix (PerkinElmer), was added to 10mL of SPO cultures and incubated with shaking for 10 minutes at 30°C ...
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bioRxiv - Cell Biology 2022Quote: LTE cell-free protein expression in combination with AlphaLISA® approach (Perkin Elmer, AUS) was performed for pairwise protein interaction analysis of PER2 and CRY2 proteins with CAVIN3 and CAVIN1 protein variants ...
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bioRxiv - Biochemistry 2023Quote: ... Bromodomain protein was co-incubated with test compounds using 384-well in AlphaPlates (PerkinElmer) in the absence or presence of DCAF16 (1 µM ...
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bioRxiv - Microbiology 2023Quote: AlphaLISA® detection kit targeted against IAV NP protein was purchased from Perkin Elmer and performed according to the manufacturers protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins on PROTEAN® TGX™ gel were transferred onto nitrocellulose membranes (Perkinelmer, NBA085A001EA) with Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: Chemiluminescence detection of the proteins was performed using Western Lightning ECL-Plus (Perkin Elmer) and an Odyssey CLx device (LI-COR) ...
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bioRxiv - Microbiology 2024Quote: ... Each collected sample was incubated with EasyTag EXPRESS-35S protein labelling mix (Perkin Elmer) at 23 µCi mL-l for 2 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... tumors (ca 120,000 cells) consisting of H460 human NSCLC transfected with luciferase and the tdTomato gene (H460 Luc+ tdTomato+, Perkin Elmer France) were injected ectopically into the left flank of 90 mice ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
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bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Reagents for iodination of proteins were obtained from the following sources: Na125I (PerkinElmer, Waltham, MA), 1,3,4,6-tetrachloro-3α,6α-diphenyl-glycouril (Iodogen® ...
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bioRxiv - Genetics 2022Quote: ... Proteins were detected using the Western Lightning Plus-ECL chemiluminescent substrate (Perkin Elmer, Cat #NEL103001EA) and autoradiograph film processed in an X-ray film developer.
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bioRxiv - Cell Biology 2020Quote: ... HA-binding protein staining was achieved with 1/100 strepavidin HRP (Perkin Elmer, Massachusetts, USA) solution for 30 min at room temperature and 3,3’-diaminobenzidine (Liquid DAB + substrate Chromagen System ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... followed by incubation with 35S-Met/Cys (30 mCi/mL EXPRESS Protein Labeling Mix, PerkinElmer) for an additional 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Viral titer was determined using p24 ELISA test measuring viral capsid protein p24 (Perkin Elmer, performed in Biomedicum Functional Genomics Unit) ...
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bioRxiv - Cell Biology 2020Quote: ... protein signals were detected via enhanced chemiluminescence (Western Lightning Plus ECL, Perkin Elmer, Waltham, MA) followed by exposure to film (CL-XPosure ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected with the chemiluminescence detection reagent Perkin Western Lightning plus ECL (Perkin Elmer) and visualized using a radiology film processor (Curix 60 ...
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bioRxiv - Microbiology 2022Quote: ... in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
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bioRxiv - Molecular Biology 2023Quote: ... in total volume of 18 μl using Protein A acceptor and Streptavidin donor beads (PerkinElmer). Varying amounts of the prefusion and postfusion form of RSV F protein in PBS supplemented with mucin (10 mg/L) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection from the membranes was performed with Western Lightning ECL Pro substrate (PerkinElmer NEL121001EA).
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was detected using the Western Lighting Plus-ECL substrate from PerkinElmer (Waltham, MA) on a G:BOX Chemi XX6 gel doc system (Syngene) ...
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bioRxiv - Immunology 2024Quote: ... The target proteins were visualized by Western Lightning PLUS-ECL or Ultra kit (Perkin Elmer). The images were acquired by Azure Biosystems C300 or Thermo Fisher Scientific iBright FL1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then pulsed with 2.20 MBq per sample with Express Protein Labeling Mix (Perkin Elmer) and incubated for 2.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the radiolabeled proteins were detected and quantified using a Cyclone Plus Phosphor Imager system (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... Cultures were labeled with 80 µCi/mL [35S] EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer) for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and protein expression signals were detected using a chemiluminescence detection kit (Perkin Elmer, Waltham, MA, USA) and visualised using a chemiluminescence imaging system (Fusion Solo S ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using Western Lighting Plus ECL detection reagent (Perkin Elmer, Waltham, MA, USA) and imaged with the ChemiDoc TouchTM system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cell Biology 2019Quote: ... detection of proteins from the membranes was performed with Western Lightning ECL Pro solution (Perkin Elmer, #NEL121001EA).
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bioRxiv - Immunology 2021Quote: Data processing was done with Elucidator software (Protein Expression Data Analysis System (Ver. 4.0.0.ror2.31, Ceiba Solutions/ PerkinElmer) for label-free quantitation based on comparison of MS1 feature volume ...
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bioRxiv - Immunology 2019Quote: ... Cells were then distributed (80 µg of proteins per well) in a 96-well microplate (Optiplate, PerkinElmer). BRET2 between RLuc8 and GFP10 was measured 1 min after addition of 5 µM coelenterazine 400a/Deep blue C (Gentaur) ...
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bioRxiv - Cell Biology 2019Quote: ... eluted proteins were incubated overnight at 4 °C and measured with a microplate reader analyzer (EnVision, PerkinElmer). Excitation spectra were obtained collecting emission at 515 ± 15 nm ...
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bioRxiv - Cell Biology 2022Quote: ... For the host protein response screen 10,000 cells/well were seeded on 96 well microplates (Perkin Elmer, CellCarrier-96 Ultra Microplates ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation of yeast cells (82) and in vivo labeling of iron-binding proteins with 55FeCl3 (Perkin Elmer) and immunoprecipitation of 55Fe/S proteins and scintillation counting were performed as published for yeast (59 ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: Leucine incorporation into proteins was assayed using 3H-Leucine (cat. # NET460001MC) obtained from Perkin Elmer (Waltham, MA), as described in a previous publication [33].
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bioRxiv - Molecular Biology 2021Quote: Groups of five oocytes were incubated with 0.5 mCi/ml EasyTag EXPRESS 35S protein labeling mix (PerkinElmer, NEG772007MC) in 100 µl complete OR-2 buffer at 23°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the separated proteins were electrophoretically transferred to a polyvinylidene difluoride (PVDF) membranes (NEF1002001PK, PerkinElmer, Waltham, MA, USA) by 400 mA for 75 minutes ...
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bioRxiv - Microbiology 2019Quote: ... and a final concentration of 125 µMci/mL of EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer, USA). Concentrations used for incubation correspond to 100- ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Streptavidin Donor beads (Cat # 6760002) and Protein A acceptor beads (Cat # AL101) were acquired from PerkinElmer (Waltham, MA).
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were detected by western blot analysis by using an enhanced chemiluminescence system (Western Lightning– ECL, PerkinElmer). Antibodies used were specific for PTEN (Cell Signaling ...