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Citations for PerkinElmer :
301 - 322 of 322 citations for Recombinant Human EGFR Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer). The signal was normalized to the relative cellular content as determined by immunoblotting of whole cell lysates probed with antibodies.
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed at room temperature 3 times 10 min in PBST before using chemiluminescence to reveal protein bands with the Western Lightning® Plus-ECL Enhanced Chemiluminescence reagent (PerkinElmer Inc.) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Ten μl of these dilutions or 10 μl of G protein storage buffer without nucleotides were pipetted into the corner of a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290). Ten 10 μl of 0.1 mM Coelenterezine 400a diluted in BRET buffer (140 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Physiology 2023Quote: ... the medium was exchanged for DMEM medium supplemented with emetine and 35S-Protein Labelling Mix (35S-Met and 35S-Cys, Perkin Elmer NEG072; 100 µCi/mL). Cells were incubated for 3 h at 37 °C ...