Labshake search
Citations for PerkinElmer :
551 - 600 of 629 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 100 μL were transferred daily (up to 10 days post dormancy induction) to a 96 well plate where 5 μCi 3H-hypoxanthine (Perkin Elmer), 5 μCi 3H-adenosine (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... Solutions were then cleared by centrifugation at 4000 g for 10 min and the supernatant analyzed in a black 96 well microtiter plate using an EnSpire (PerkinElmer, USA) microplate reader with an Excitation/Emission wavelength of 630 nm/ 660 nm (with 9 nm band-passes around the maximum) ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... bright field and digital phase contrast images of 10-15 fields per well were captured on an Operetta automated microscope (Perkin Elmer) at 30 min intervals for approximately 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... The plate was shaken at 300 RPM at room temperature for 10 minutes after which RLUs were read on an EnSight (Perkin Elmer) microplate reader ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 minutes at room temperature and the luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Immunology 2021Quote: Vero-E6 were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20’000 cells per well ...
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bioRxiv - Neuroscience 2021Quote: ... was added to the chamber for 30 min to inhibit transcription after which slices were transferred to fresh aCSF containing ~10 mCi/ml [35S] Met/Cys (Perkin Elmer) for an additional 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CCK hybridization was revealed by a TSA reaction using fluorescein isothiocyanate (FITC)-labeled tyramide (1:100 for 10 minutes, NEL741001KT Perkin Elmer). After several washes ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Cell Biology 2019Quote: ... mounted on a TMC isolation platform (Technical Manufacturing Corporation) with a Yokogawa CSU 10 spinning disc head and Hamamatsu C9100-13 ImagEM EMCCD camera (Perkin Elmer) with diode lasers of 491 nm ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with an anti-ERα antibody (F-10) overnight followed by Cy3 Tyramide signal amplification (PerkinElmer-TSA-Plus-Cy3) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... was incubated with oligonucleotide probes (0.01 pmol-10 fmol) 5’-radiolabeled with [y-32P]ATP (5000 Ci/mmol; PerkinElmer Life Science). Different dsDNA structures were prepared by annealing the radiolabeled sense oligonucleotides with a twofold excess of unlabeled complementary oligonucleotide and purified on non-denaturing polyacrylamide gels ...
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bioRxiv - Immunology 2021Quote: Vero-E6 and Vero E6-TMPRSS2 were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 2e4 cells per well ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: Vero E6 expressing TMPRSS2 or not were grown in DMEM supplemented with 10% FBS and seeded into white 96 well plates (PerkinElmer, 6005688) at a density of 20 thousand cells per well ...
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bioRxiv - Immunology 2021Quote: ... The plates were shaken at 300 rpm at room temperature for 10 minutes and relative light units (RLUs) were then read on an EnSight microplate reader (Perkin Elmer). Percent neutralization was determined by subtracting the mean background (uninfected cells with luciferase substrate alone ...
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bioRxiv - Immunology 2021Quote: ... incubated for 10 minutes at room temperature and the luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... Image analysis was performed on the maximum projection image of 10 whole-well z-planes of the confocal imaging using Harmony software (Perkin Elmer).
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
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bioRxiv - Plant Biology 2023Quote: ... Stained SDS-PAGE bands were excised and transferred to scintillation vials and emulsified in 10 ml of Ultima Gold XR scintillation liquid (Perkin Elmer) in darkness at room temperature overnight ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were incubated for 10 min on a shaker in the dark before measuring luminescence on the EnVision 2102 Multilabel reader (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: Glassware and plasticware used for RNA gel electrophoresis were soaked in 10% bleach for 5 minutes before washed by COUNT-OFF™ Surface Cleaner (PerkinElmer) and liberally rinsing in double-distilled water ...
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bioRxiv - Cancer Biology 2023Quote: ... We verified that these cells did not lose viability after overnight culture in serum-free medium relative to medium with 10% serum as determined by cell-based measurements of CBG bioluminescence in an IVIS Lumina (Perkin Elmer). We injected 1 × 105 breast cancer cells per mouse (n = 5 per each cell type) ...
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bioRxiv - Neuroscience 2023Quote: ... in the presence of 7 increasing concentrations (0.15 – 10 nM) of [3H]-raclopride (specific activity 82.8Ci/mmol, Perkin Elmer, #NET975250UC, Canada) for D2 binding ...
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bioRxiv - Immunology 2023Quote: ... Iodine-125 (125I) labeling was performed by incubating DTPA-bsAbs in iodogen coated Eppendorf tubes with Na125I (10−5 M NaOH, Perkin Elmer) in 0.5 M phosphate buffer (pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were carefully added and plates were incubated for 10 min before luminescence reading in the EnVision 2103 Multilabel Reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were briefly vortexed and added to 20 mL liquid scintillation vials to which 10 mL of Ultima Gold was added (Perkin Elmer). The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated in the dark for 10-15 minutes before luminescence was read on a Victor3V 1420 Multilabel Counter (Perkin Elmer).
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bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng was used for random priming reactions in the presence of the radiolabeled nucleotide [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml, Perkin Elmer) using a random primer DNA labeling kit (TaKaRa ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 10 minutes at room temperature before images were taken using IVIS Spectrum In Vivo Imaging System (Perkin Elmer). Wnt-activation signal was calculated as the bioluminescent signal captured by the IVIS Spectrum normalized to the tumor volume recorded per animal ...
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Biophysics 2023Quote: Laser surgery was performed on a Zeiss Axio Imager M2m microscope equipped with a Yokogawa CSU-10 spinning disk confocal head (Perkin Elmer), a Hamamatsu EM-CCD camera and a Zeiss 40X ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes before changing to MOPS-buffered ‘Air medium’ with 10% serum but lacking luciferin and moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Cancer Biology 2021Quote: ... the mice underwent BLI beginning at 10 days after implantation and every week thereafter with the In Vivo Imaging System (IVIS, PerkinElmer, Waltham, MA), and luminescence flux was recorded to assess tumor growth ...
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bioRxiv - Cell Biology 2020Quote: ... was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer, USA). Measurements were done at least in triplicates and relative luciferase units (RLU ...