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Citations for PerkinElmer :
201 - 250 of 514 citations for Recombinant Human CD79B protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown until OD600 ∼ 0.02 and labeled with 50 μCi/ml 32P-orthophosphate (900 mCi/mmol; PerkinElmer). When OD600 reached 0.2-0.4 ...
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bioRxiv - Microbiology 2020Quote: ... was used for hybridization with oligonucleotides 5’-end labeled with 6,000 Ci/mmol γ-32P ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled using an 35S Met/Cys labeling mixture at 200 µCi/ml (EasyTag Express35S; Perkin Elmer) for 30 minutes in media that contained drug treatments and that was 20 percent MEF culture medium as above and 80 percent DMEM lacking Met/Cys and with dialyzed FBS ...
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bioRxiv - Biophysics 2019Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Bioengineering 2020Quote: MTs were transferred with a tip quickly coated in fetal calf serum (FCS) into a CellCarrier-384 Ultra Microplates (6057300, Perkin Elmer). They were then fixed in 4% PFA (P6148 ...
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bioRxiv - Microbiology 2023Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Biophysics 2024Quote: Single-point FCS data were acquired with an Insight Cell confocal microscope (Evotec Technologies, Hamburg, Germany, now PerkinElmer, Waltham, MA, USA). Excitation was performed using a 488 nm continuous wave solid state diode-pumped laser (Sapphire 488-20/460-10 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Biochemistry 2019Quote: ... Oligonucleotides were 5’-end-labeled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1; Perkin Elmer) using polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... primer (5’-AGUUGUUGAUC) was 32P-labeled at the 5′ position upon incubation with [γ-32P] ATP (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with canine rough microsomes (tRNA probes, College Station, TX) and EasyTag 35 S-labeled methionine (PerkinElmer, Waltham, MA) for one hour at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were labeled with 250-1000 µCi/ml of [35S]-Express label (1100 Ci/mmol; PerkinElmer, Boston, MA) at 37°C depending on the length of the pulse ...
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bioRxiv - Immunology 2021Quote: ... The 3H-labeled products were isolated using DEAE-Sephadex A-50 columns and quantitated by liquid scintillation counting (PerkinElmer). Non-isotopic methylated RNA fragments were prepared using cold SAM (Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Molecular Biology 2024Quote: ... from synthetic oligonucleotides (0.2 µM) and trace-labeled with 32P alpha-ATP (800 Ci/mmol, 12.5 µM, Perkin Elmer) in a 20 µl reaction according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Cell Biology 2021Quote: Primary human hepatocytes were seeded in 96-well black CellCarrier plates (PerkinElmer) at a density of 5×103 cells per well ...
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bioRxiv - Immunology 2021Quote: Human fibroblasts cells were seeded on CellCarrier-96well Ultra microplate (Perkin Elmer) and incubated for at least 24 hours in growth media ...
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bioRxiv - Biochemistry 2023Quote: 5x104 human neutrophils were seeded in a black 96-well plate (PerkinElmer) in HyClone HBSS +Ca ...
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bioRxiv - Immunology 2020Quote: ... The IgGs and ACE2 were incubated in the plates for 1 hour and ACE2-Fc-Biotin was detected by DELFIA Eu-N1 Streptavidin (Perkin Elmer, 1244-360) using the DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated with a mixture of [3H]-labeled steroids (final concentration, 10 nM T and 10 nM progesterone; ~1,000,000 cpm/well; PerkinElmer, Waltham, MA) at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... A series of incubations was then performed to amplify the signal of the hybridized probe and label target probes for the assigned fluorescence detection channel (target probe was labeled for the assigned fluorescence detection channels Opal 520, Opal 570, Opal 650 and Opal 780, PerkinElmer). Nuclei were stained using a DAPI nuclear stain (RNAscope ref 323108 ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... was next added to the cultures and cells were labeled for 2 min with [2,8-3H]-adenine (NET06300 Perkin Elmer) at 0 ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µL of RNA was 3’-end-labeled with 32pCp (prepared by incubating 16.5 µl of γ-32P-ATP (PerkinElmer), 1 µl of T4 polynucleotide Kinase ...
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bioRxiv - Molecular Biology 2022Quote: ... Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer, 10mCi/ml) using PCR products containing an upstream T7 promotor (see DNA constructs) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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bioRxiv - Cell Biology 2023Quote: ... The biodistribution of the fluorescently labeled nanoalgosomes was determined by in vivo near-infrared fluorescence imaging using an IVIS Spectrum scanner (IVIS Spectrum CT; PerkinElmer). Athymic nude mice (n=6 ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Molecular Biology 2024Quote: ... sgRNA flanking sequences are: TCGAGCATTATATGAAC-sgRNA-GGGTATTGGGGAATTCATTATGC) was labeled with 32P gamma-ATP (6000 Ci/mmol, 25 µM, Perkin Elmer) with PNK (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The 100-bp mini-circle was prepared as follows: oligos cb1027 and cb1028 were labeled with [γ-32P]-ATP (Perkin Elmer) by T4 polynucleotide kinase ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... For the analysis of Dscam and elav alternative splicing primers were labeled with 32P gamma-ATP (6000 Ci/ mmol, 25 mM, Perkin Elmer) with PNK to saturation and diluted as appropriate 69 ...
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bioRxiv - Cell Biology 2019Quote: ... 3 OD600 units of early log phase cells were labeled with 80 mCi of L-[35S]-methionine/cysteine mix (Perkin Elmer). Immunoprecipitated proteins were separated on SDS-PAGE and exposed to phosphor screens ...
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bioRxiv - Developmental Biology 2019Quote: ... were used to detect labeled probes by fluorophore deposition (Fluorescein or Cyanine 3) using the TSA Plus System (Perkin Elmer, NEL753001KT). Nuclei were stained with Hoechst 33342 (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes (listed in Supplementary Table S2) were 5′ 32P-end labeled with 0.3 mCi of γ-32P ATP (Perkin Elmer) by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs ...