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Citations for PerkinElmer :
201 - 250 of 319 citations for Recombinant Human BTLA Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Transformation of yeast cells (82) and in vivo labeling of iron-binding proteins with 55FeCl3 (Perkin Elmer) and immunoprecipitation of 55Fe/S proteins and scintillation counting were performed as published for yeast (59 ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: Leucine incorporation into proteins was assayed using 3H-Leucine (cat. # NET460001MC) obtained from Perkin Elmer (Waltham, MA), as described in a previous publication [33].
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bioRxiv - Molecular Biology 2021Quote: Groups of five oocytes were incubated with 0.5 mCi/ml EasyTag EXPRESS 35S protein labeling mix (PerkinElmer, NEG772007MC) in 100 µl complete OR-2 buffer at 23°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the separated proteins were electrophoretically transferred to a polyvinylidene difluoride (PVDF) membranes (NEF1002001PK, PerkinElmer, Waltham, MA, USA) by 400 mA for 75 minutes ...
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bioRxiv - Microbiology 2019Quote: ... and a final concentration of 125 µMci/mL of EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer, USA). Concentrations used for incubation correspond to 100- ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Streptavidin Donor beads (Cat # 6760002) and Protein A acceptor beads (Cat # AL101) were acquired from PerkinElmer (Waltham, MA).
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were detected by western blot analysis by using an enhanced chemiluminescence system (Western Lightning– ECL, PerkinElmer). Antibodies used were specific for PTEN (Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: ... 2020 submitted): namely in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
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bioRxiv - Neuroscience 2022Quote: ... 25-30 μg of membrane protein was combined with a fixed concentration (0.57-5.18 nM) of 3H-diprenorphine (PerkinElmer) and concentration curves of KUNB106 or positive control (naloxone for mu and delta ...
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bioRxiv - Microbiology 2020Quote: ... Protein localization in fungal cells was carried out by an UltraVIEW VoX Spinning Disk Confocal Microscope (PerkinElmer, USA) and a Leica DMi8 (Leica ...
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bioRxiv - Cell Biology 2021Quote: ... Viral protein expression was detected by imaging with Opera LX and Phenix high content imaging platforms (Perkin Elmer) and the number of infected cells determined using a Columbus Analysis system (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30-40 μg of membrane protein was incubated with varying concentrations of compound and 0.3 nM [3H]-CP55,940 (PerkinElmer) for 90 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
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bioRxiv - Cancer Biology 2021Quote: Protein synthesis assays were performed by measurement of the incorporation of 3H-Leucine (Perkin Elmer, Waltham, MA USA) into protein ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein-bound [3H]ryanodine was separated by filtration through polyethyleneimine-treated glass filters (Filtermat B; PerkinElmer, Waltham, MA) using a Micro 96 Cell Harvester (Skatron Instruments ...
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bioRxiv - Immunology 2023Quote: ... 10 µl of Protein A AlphaLISA® Acceptor beads (10 µg/ml final concentration; Perkin Elmer cat# AL101C) were added ...
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bioRxiv - Biophysics 2023Quote: The ATPase activities of the protein samples were measured with a UV/Vis Spectrometer (Lambda 35, Perkin Elmer) using an ATP (2 mM ...
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bioRxiv - Physiology 2023Quote: ... Protein content was estimated using a diode array NIR spectrometer (DA-7200, Perten Instruments (today PerkinElmer, Waltham, USA)).
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bioRxiv - Cancer Biology 2023Quote: ... BioRad) and protein bands were visualized by Western Lightning Plus ECL system (Catalog # ORT2655 and ORT2755, Perkin Elmer).
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
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bioRxiv - Neuroscience 2021Quote: ... CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer) were transiently transfected with ckr-1/pcDNA3.1 ...
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bioRxiv - Immunology 2019Quote: ... Acquired images were used to build spectral library of all the target proteins using inForm Cell Analysis software (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... Samples were prepared in a 96-well plate using the manufacturer “HT Protein Express Assay Sample Buffer” (PerkinElmer, USA) supplemented with 36.4 mM (final concentration ...
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bioRxiv - Genomics 2021Quote: ... Percentage of protein content in the grain was measured using a Perten DA 7000 NIR analyzer (Perkin Elmer, Sweden).
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bioRxiv - Microbiology 2021Quote: ... The final Western blotting bands were visualized using an enhanced chemiluminescence protein detection system (PerkinElmer Life Sciences, Boston, MA).
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... shRNA expression with surrogate red fluorescent protein (RFP) was tracked by fluorescence (IVIS Spectrum In Vivo Imaging Station, PerkinElmer). For chloroquine xenograft experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by a high content screening system (Operetta Enspire, Perkin Elmer, USA). The integral optical density (IOD ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then supplemented with 7.4 MBq of [35S] methionine and [35S] cysteine (EXPRE35S35S Protein Labeling Mix, [35S]-, PerkinElmer) and incubated for 1 h to specifically label newly synthesized mitochondrial proteins ...
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bioRxiv - Immunology 2020Quote: ... The cpms of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We next added 0.8 μl of FLAG-GST-transcription factor proteins to 384-well AlphaPlates using a NanoHead (PerkinElmer) and a Janus Workstation (PerkinElmer) ...
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bioRxiv - Microbiology 2019Quote: ... and the protein band reacting with the antiserum was detected using a chemiluminescence detection system (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Physiology 2022Quote: ... 60-100 µg protein was incubated with 100 μl TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1 ...
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bioRxiv - Neuroscience 2022Quote: GPCR activation assays were performed using CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer). The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...