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Citations for PerkinElmer :
1 - 50 of 89 citations for Recombinant Autographa Californica Multiple Nucleopolyhedrovirus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Absorbance was read at 450nm using EnSight multiple plate reader (PerkinElmer). Data are expressed as neutralization curves calculating the percentage of ACE2/RBD binding inhibition at the different serum dilutions relative to the control without inhibitory antibodies.
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bioRxiv - Developmental Biology 2022Quote: ... Organoids were then transferred into multiple CellCarrier-96 Ultra Microplates (PerkinElmer, 6055300) for staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Organoids were then transferred into multiple CellCarrier-96 Ultra Microplates (PerkinElmer, 6055300) for staining ...
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bioRxiv - Immunology 2020Quote: ... Absorbance was read at 405 and 620 nm using EnSight multiple plate reader (PerkinElmer). The endpoint titer was defined as the highest serum dilution that resulted in an absorbance value (OD-optical density ...
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bioRxiv - Cancer Biology 2023Quote: ... the entire area was captured using multiple images and stitched using the in-built Harmony software (Perkin Elmer).
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bioRxiv - Cancer Biology 2021Quote: ... in DMEM and multiple fields and z-stacks were imaged on the Opera-Phenix High-Content Screening System (PerkinElmer).
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bioRxiv - Cancer Biology 2021Quote: ... Multiple fields and z-stacks were imaged for each well using the Opera Phenix High-Content Screening System (PerkinElmer). Maximal projection was used to reconstruct representative Z-stack fluorescent confocal images of 25 fields with 30-35 Z-stack images spaced every 55 μm using the Harmony High-Content Imaging and Analysis Software (PerkinElmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... and multiple fields and z-stacks were imaged for each well using the Opera Phenix High-Content Screening System (PerkinElmer). Maximal projection of all imaged fields and z-stacks were reconstructed using the Harmony High-Content Imaging and Analysis Software (PerkinElmer ...
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bioRxiv - Microbiology 2021Quote: ... Image acquisitions of multiple fields (9 to 25) per well were performed on an automated confocal microscope (OPERA Phenix, Perkin Elmer) using 60X objective ...
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bioRxiv - Cell Biology 2023Quote: NMuMG cells expressing either EGFP-BIK-L61G or EGFP-PUMA4E were seeded (3,000 cells per well) in multiple wells (at least 3) of a 384-well microplate (CellCarrier-384 ultra, B128 SRI/160; Perkin Elmer) and allowed to grow for 24 h before staining with the nuclear dye DRAQ5 (5nM ...
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was mixed with Ultima Gold (Perkin Elmer) and the relative expression of Na+-dependent uptake of radiolabeled glutamate was calculated based on the measurement with a microplate scintillation counter (1450 MicroBeta Plus ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were placed into a white 96-well plate (Perkin Elmer) to assess Luciferase and Renilla activities using Envision Multilabel plate reader (Perkin Elmer) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was transferred to light grey 384-well untreated AlphaPlates (PerkinElmer) by adding 10 µl per well ...
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bioRxiv - Biochemistry 2023Quote: ... and the remaining lysate was mixed with Optiphase HiSafe 3 (PerkinElmer). The radioactivity was monitored using a β-scintillation counter (LSC-8000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell lysates were combined with 8mL Ultima Gold scintillation cocktail (PerkinElmer 6013329) and CPM was measured using Beckman LS6500 Scintillation counter ...
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bioRxiv - Microbiology 2022Quote: ... ESAs and lysates were plated in white-bottom assay plates (PerkinElmer #6002290) and incubated at room temperature for 5 min with substrate-containing assay solution before detecting luciferase signal using a Biotek Cytation3 multimode reader ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were transferred to a 384 OptiPlate (Perkin Elmer, Waltham, MA, USA) and mixed 1:1 with antibody mixture from the IP-ONE-Gq kit ...
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bioRxiv - Biochemistry 2019Quote: ... cell lysate (5 μl/well) was transferred to a 384-well ProxiPlate (PerkinElmer) and added to the donor and acceptor reaction mix (2.5 μl/ well ...
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bioRxiv - Immunology 2022Quote: ... 110 μl of the cell lysate was transferred to a black/white plate (PerkinElmer). Luminescence was measured using a PerkinElmer Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Four μL of cell lysate was transferred to a 384 well ProxiPlate (Perkin Elmer). Immediately prior to use ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µl of lysates were manually transferred to a 384-well opaque OptiPlate (Perkin Elmer) in quadruplicate wells and supplied with APC-POM1 and EU-POM2 (5 and 2.5 nM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... using 10µl lysate in a black OptiPlate 96-well plate (6005279, Perkin Elmer, Waltham, MA) and 50 μL homemade firefly and luciferase reagents (according to (Fuerer et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell lysate (5 μL/well) was transferred to a 384-well ProxiPlate (PerkinElmer, Melbourne, Australia) and added to the donor and acceptor reaction mix (2.5 μL/ well ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were incubated with 1 mM GTP/[α-32P]GTP (0.1 μCi/μl) (PerkinElmer) and incubated at 30°C for various time intervals for comparison pYfiN and pYfiN(Ips) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Equal volumes of lysate were then subjected to analysis using an EnSpire Multimode Plate Reader (PerkinElmer).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of the cell lysates were transferred into a white 384 well plate (ProxiPlate, PerkinElmer). 5 μL of the Europium-cAMP tracer (1 to 50 dilution of Eu-tracer stock in cAMP detection buffer ...
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bioRxiv - Physiology 2023Quote: ... by adding 200 μl of muscle lysate to 4 ml scintillation fluid (Ultima Gold, Perkin Elmer). Glucose uptake rates were calculated as described in 14.
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bioRxiv - Physiology 2024Quote: ... by adding 200 μL of muscle lysate to 4 mL scintillation fluid (Perkin Elmer, Ultima Gold). Glucose uptake rates were calculated as described previously 20.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer), and the other half was used for immunoblotting.
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL cell lysate was added to 3 mL of Ultima Gold scintillation liquid (Perkin Elmer #6013329). Samples were kept in the dark overnight and 3H-2DG uptake was measured the following day on a Hidex 300 SL scintillation counter ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mL of the cell lysate was counted in a liquid scintillation counter (TRI-CARB 4910TR, PerkinElmer) and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Cell Biology 2020Quote: ... Absorbance of the lysates was measured at 420 nm using a spectrophotometer (EnSpire Multimode Plate Reader, Perkin Elmer).
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were transferred into vials and measured using a gamma counter (Wallac 1470 Wizard, PerkinElmer, Waltham, MA, USA). The saturation assay was performed by adding 100 μL of binding buffer with increasing amounts of 125I-NDP-MSH to the cells in the presence or absence of 1 μM of unlabeled NDP-MSH.
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was quantified by measuring luciferase activity in cell lysates using Britelite Plus (Perkin Elmer, Richmond, California) and following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... 20 µL of the lysate was transferred in duplicate in a well of an OptiPlate-96 HB (PerkinElmer) and 100 µL of Luciferase Assay Reagent II was added in each well ...
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bioRxiv - Immunology 2023Quote: ... equal amounts of lysate (six replicates per condition) were spotted onto a 96-well DEAE filter mat (PerkinElmer) and the mat was dried at 60°C ...
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bioRxiv - Cell Biology 2023Quote: ... the rabbit reticulocyte lysate master mix was supplemented with EasyTag L-35-Methionine (4% final reaction volume; PerkinElmer). Canine rough pancreatic microsomes (cRMs ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...