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Citations for PerkinElmer :
301 - 350 of 1544 citations for Rat Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed in TBST at least 3 times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839).
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bioRxiv - Biophysics 2021Quote: ... GTP loading was initiated by addition of a premixture of cell membranes and WGA-SPA PVT beads (PerkinElmer, Waltham, MA) to a final concentration of 0.25mg beads/well and 15,000cpm 5-HT5AR-Gi1/well (as determined by [3H]-LSD binding) ...
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bioRxiv - Neuroscience 2019Quote: ... membranes were incubated with increasing free concentrations of [3H] SCH 23390 (0.02 nM to 10 nM, PerkinElmer, Boston, MO, USA) or [3H]R-a-methyl histamine (0.1 nM to 20 nM [3H]RAMH ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were then rinsed in TBST three times and then treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the membrane was transferred to a fresh falcon tube and incubated with 1ml of Western LightningPlus-ECL chemiluminescent reagent (PerkinElmer). Proteins bands were visualised with an iBright instrument (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were washed 5 min 3 times and developed using enhanced chemiluminiscence reagent (Western Lightning Plus-ECL; Perkin Elmer). The following primary antibodies were used ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
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bioRxiv - Microbiology 2022Quote: Radioactivity of cell wall and membrane isolates was measured by scintillation counting using a Liquid Scintillation Analyzer Tri-Carb 4910 TR (PerkinElmer). To do this ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Developmental Biology 2023Quote: ... membrane staining was added to single wells immediately before imaging on an Opera Phenix™ High-Content Screening System (PerkinElmer).
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bioRxiv - Biophysics 2020Quote: Protein concentrations were determined on a Lambda 40 UV/VIS spectrophotometer (PerkinElmer Instrument) using the extinction coefficients of 6,990 M-1cm-1 for CI2 and 15,470 M-1cm-1 for ACBP ...
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bioRxiv - Bioengineering 2022Quote: ... Protein A-conjugated acceptor beads (#6760617C, PerkinElmer©; 10 μL, 50 μg/mL) were then added to the vials ...
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bioRxiv - Microbiology 2020Quote: ... Spectraplate-384 plates with high protein binding treatment (custom made from Perkin Elmer) were coated overnight at 4 °C with 0.5 µg/ml (for mAbs ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins of interest were visualized by Western Lightening Plus ECL (NEL103001EA, Perkin Elmer). NIH Image J was used to analyze protein levels ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were detected using Western Lightening Plus ECL (NEL103001EA, Perkin Elmer). NIH Image J was used to quantify protein levels by analyzing the scanned grey-scale films ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Proteins were detected by ECL and visualized by chemiluminescence (Perkin Elmer, Waltham, MA) using the Bio-Rad Chemidoc Imaging system (Hercules ...
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bioRxiv - Physiology 2020Quote: ... Proteins were detected using ECL and visualized by chemiluminescence (Perkin Elmer, Waltham, MA) using the BioRad Chemidoc Imaging system (Hercules ...
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bioRxiv - Molecular Biology 2022Quote: ... [35S]-Met/Cys was added (30 μCi/mL EXPRE35S Protein Labeling Mix, PerkinElmer) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... and target proteins were detected using Western Lightning Plus-ECL (PerkinElmer, Waltham, MA) and a Chemidoc imaging system (Biorad) ...
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bioRxiv - Molecular Biology 2023Quote: ... indicated proteins were expressed and radiolabeled with EasyTagTM L-[35S]-Methionine (Perkin Elmer) using the TnT Coupled Reticulocyte Lysate System (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... The detected proteins were visualized via the Plus-ECL method (PerkinElmer, Waltham, USA). To quantify proteins of interest ...
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bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Biophysics 2021Quote: ... add 10 mL scintillation solution and measured the radioactivity retained on the membranes in liquid scintillation counter Tri-carb 4910TR (Perkin Elmer) [44].
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bioRxiv - Plant Biology 2020Quote: ... 10.5 μg of gDNA was digested with the restriction enzymes EcoRV and XbaI from New England Biolabs (http://www.neb.com) and blotted onto a nylon membrane (GeneScreenPlus; Perkin Elmer, http://www.perkinelmer.com) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
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bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was separated in 2% glyoxal agarose gels and blotted onto a positively charged nylon membrane (GeneScreen Plus, Perkin Elmer). [32P]-labelled probes specific for each transcript were generated by linear PCR ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer). OD600 of cell-free medium was removed from all cell density values ...
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bioRxiv - Evolutionary Biology 2019Quote: ... followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents, Perkin Elmer TSA Plus Cy3 or Cy5 Kit). Residual enzyme activity was inhibited via 45-minute incubation in 0.1% hydrogen peroxide in PTW followed by four PTW washes prior to addition and development of the second peroxidase-conjugated antibody76.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...
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bioRxiv - Cell Biology 2020Quote: ... metabolically labelled by supplementing starvation medium with35S-Met/Cys (EXPRE35S35S Protein Labelling Mix (PerkinElmer), 80 μCi/6 cm plate ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labelled by adding the EXPRE35S35S Protein Labeling Mix (PerkinElmer EasyTagTM NEG772) at a ratio of 9 μL per mL of the concentrated culture ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were detected using enhanced chemiluminescence (Western Lightning Plus-ECL; Perkin Elmer, Waltham, MA). The antibodies used in the assay are listed in the antibody section.
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bioRxiv - Cell Biology 2021Quote: ... Protein detection was performed using Super Signal chemiluminescent ECL-plus (PerkinElmer, Waltham, MA, USA) on a BioRad Gel doc (Bio Rad ChemiDoc Touch ...
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bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... To metabolically label the cells 5uL of EasyTag EXPRESS 35S protein labeling mix (PerkinElmer), was added to 10mL of SPO cultures and incubated with shaking for 10 minutes at 30°C ...
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bioRxiv - Cell Biology 2022Quote: LTE cell-free protein expression in combination with AlphaLISA® approach (Perkin Elmer, AUS) was performed for pairwise protein interaction analysis of PER2 and CRY2 proteins with CAVIN3 and CAVIN1 protein variants ...
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bioRxiv - Biochemistry 2023Quote: ... Bromodomain protein was co-incubated with test compounds using 384-well in AlphaPlates (PerkinElmer) in the absence or presence of DCAF16 (1 µM ...
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bioRxiv - Microbiology 2024Quote: ... Each collected sample was incubated with EasyTag EXPRESS-35S protein labelling mix (Perkin Elmer) at 23 µCi mL-l for 2 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: Chemiluminescence detection of the proteins was performed using Western Lightning ECL-Plus (Perkin Elmer) and an Odyssey CLx device (LI-COR) ...