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Citations for PerkinElmer :
1 - 50 of 1247 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... the animals were anesthetized with 5 mM Levamisole and imaged using 488 nm laser excitation with a Yokogawa CSU-X1-A3 spinning disc confocal head and a Hamamatsu C9100-13 EMCCD Camera controlled by Volocity collectively supplied by PerkinElmer. Imaging was done under 100X 1.4 NA objective using 300 ms exposure at 3 frames/second for a total of 3 minutes for juIs338 and 600 ms exposure at 1.6 frames/second for a total of 3 minutes for wyIs291 ...
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bioRxiv - Biophysics 2021Quote: ... using a 100X/1.4 NA oil objective on the Olympus IX83 microscope integrated with the Yokogawa CSU-X1-A3 spinning disc and Hamamatsu ImagEM C9100-13/14 EMCCD Camera (by Perkin Elmer). The analysis for vesicle accumulation following axotomy was conducted as follows-
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Genomics 2020Quote: ... secondary antibody goat anti-rat and Opal 520 (fp1487, PerkinElmer). After the third staining ...
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bioRxiv - Neuroscience 2021Quote: ... CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer) were transiently transfected with ckr-1/pcDNA3.1 ...
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bioRxiv - Neuroscience 2022Quote: GPCR activation assays were performed using CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer). The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labelled by adding the EXPRE35S35S Protein Labeling Mix (PerkinElmer EasyTagTM NEG772) at a ratio of 9 μL per mL of the concentrated culture ...
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bioRxiv - Genetics 2022Quote: ... To metabolically label the cells 5uL of EasyTag EXPRESS 35S protein labeling mix (PerkinElmer), was added to 10mL of SPO cultures and incubated with shaking for 10 minutes at 30°C ...
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bioRxiv - Cell Biology 2022Quote: LTE cell-free protein expression in combination with AlphaLISA® approach (Perkin Elmer, AUS) was performed for pairwise protein interaction analysis of PER2 and CRY2 proteins with CAVIN3 and CAVIN1 protein variants ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then pulsed with 2.20 MBq per sample with Express Protein Labeling Mix (Perkin Elmer) and incubated for 2.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Neuroscience 2021Quote: Confluent rat astrocytic cultures were incubated with 100 nCi [5,6,8,9,11,12,14,15-3H]-AA (PerkinElmer) overnight before the experiments ...
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bioRxiv - Synthetic Biology 2022Quote: ... Bioluminescence imaging of rats was proceeded using an IVIS Spectrum system (PerkinElmer, USA) at 24h and 96h after the injection ...
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bioRxiv - Physiology 2020Quote: ... rats received 5 nmol Cat K 680 FAST (CatK) (PerkinElmer, Waltham, MA, USA). CatK is an activatable fluorescent probe that detects in vivo Cathepsin K activity ...
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bioRxiv - Immunology 2019Quote: ... Cells were then distributed (80 µg of proteins per well) in a 96-well microplate (Optiplate, PerkinElmer). BRET2 between RLuc8 and GFP10 was measured 1 min after addition of 5 µM coelenterazine 400a/Deep blue C (Gentaur) ...
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bioRxiv - Cell Biology 2022Quote: ... For the host protein response screen 10,000 cells/well were seeded on 96 well microplates (Perkin Elmer, CellCarrier-96 Ultra Microplates ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation of yeast cells (82) and in vivo labeling of iron-binding proteins with 55FeCl3 (Perkin Elmer) and immunoprecipitation of 55Fe/S proteins and scintillation counting were performed as published for yeast (59 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Microbiology 2020Quote: ... Protein localization in fungal cells was carried out by an UltraVIEW VoX Spinning Disk Confocal Microscope (PerkinElmer, USA) and a Leica DMi8 (Leica ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Immunology 2019Quote: ... Acquired images were used to build spectral library of all the target proteins using inForm Cell Analysis software (PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then supplemented with 7.4 MBq of [35S] methionine and [35S] cysteine (EXPRE35S35S Protein Labeling Mix, [35S]-, PerkinElmer) and incubated for 1 h to specifically label newly synthesized mitochondrial proteins ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pulse-labeled for the indicated times with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). After the pulse ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
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bioRxiv - Cell Biology 2023Quote: ... BMK-dko cells stably expressing a mCerulean3-fused anti-apoptotic protein were seeded at 4000 cells/well into a CellCarrier-384 Ultra Microplate (PerkinElmer). 24 hours later ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Bioengineering 2021Quote: Scaffolds seeded with EPCs in the ZDF rats were monitored via In Vivo Imaging Spectrum (IVIS) system (PerkinElmer) at designated time points (1 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... background luminescence was measured for each rat (1 sec exposure, field of view D, PerkinElmer IVIS, Waltham, MA, USA). Both knees were then injected with either 50 μL NanoLuc™ Luciferase (NL ...
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bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...