Labshake search
Citations for PerkinElmer :
251 - 300 of 340 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... slides were washed in TNT and treated with Fluorescein Amplification Reagent (Perkin Elmer, 1:50 in Plus Amplification Diluent) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 20 μl of a 1:1 mix of Western Lightning oxidizing and enhanced luminol reagents (Perkin Elmer Life Sciences) was added to each well ...
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bioRxiv - Physiology 2021Quote: ... acylcarnitines were derivatized to their butyl esters and treated with the reagents of the NeoGram MSMS-AAAC kit (PerkinElmer). Their analysis was carried out on a Waters 2795/Quattro Micro AP liquid chromatography–tandem mass spectrometer (Waters ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight and detected using HRP-conjugated secondary antibodies and chemiluminescence with the Western Lightning Plus-ECL Reagent (Perkin Elmer).
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bioRxiv - Cancer Biology 2021Quote: ... The tissue was then blocked for 30 min using TNB blocking buffer (0.1 M TRIS-HCl, pH 7.5; 0.15 M NaCl; 0.5% TSA Blocking Reagent (PerkinElmer, FP1012 or FP1020)) ...
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bioRxiv - Immunology 2022Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Immunohistochemistry was performed in Tris/NaCl blocking buffer (0.1□M Tris-HCl, 0.15□M NaCl, 5□μg/ml blocking reagent (Perkin Elmer), pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... The slides were blocked in TNB buffer (0.1 M Tris-HCl pH 7.5, 0.15 M NaCl, 0.5% Blocking Reagent; PerkinElmer, Waltham, MA, USA) at RT for 45 min ...
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bioRxiv - Immunology 2021Quote: ... Results were read after 48 hours using the EnSight Multimode Plate Reader and BriteLite Plus Luciferase reagent (PerkinElmer, USA). The values were normalized ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed and luciferase activity was determined using a microtiter plate luminometer and BriteLite Plus Reagent (Perkin Elmer). Neutralization titers were defined as the sample dilution at which relative luminescence units (RLU ...
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bioRxiv - Cancer Biology 2023Quote: ... Multispectral images were unmixed using spectral libraries built from images of single stained tissues for each reagent using the inForm Advanced Image Analysis software (inForm 2.4.6; PerkinElmer/Akoya). A selection of 10-15 representative multispectral images spanning all nine tissue sections was used to train the inForm software (tissue segmentation ...
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bioRxiv - Cell Biology 2022Quote: ... After thorough wash with TBST for 30 min membranes were treated with Lightning™ Plus Chemiluminescence Reagent (PerkinElmer, NEL104001EA) after mixing the Enhanced Luminol Reagent and the Oxidizing Reagent 1:1 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were then rinsed in TBST 3 times and treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA). Membranes were exposed to GE Healthcare Hyperfilm ECL (28906839 ...
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bioRxiv - Cancer Biology 2023Quote: ... Dehydrated coverslips were overlaid with 0.3 μg/ml TAMRA–OO-(CCCTAA)3 or 0.3 μg/mL Alexa 488–OO-(CCCTAA)3 telomeric PNA probe (Panagene) in PNA hybridization solution (70% deionized formamide, 0.25% (v/v) NEN blocking reagent (PerkinElmer), 10 mM Tris–HCl ...
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bioRxiv - Physiology 2023Quote: ... and its integrity was assessed using 5K / RNA / Charge Variant Assay LabChip and RNA Assay Reagent Kit (Perkin Elmer). Libraries were generated from 250 ng of total RNA as following ...
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bioRxiv - Microbiology 2023Quote: ... 100uL of media was removed from the plate and incubated with 100 uL Britelite Plus reagent (PerkinElmer, Waltham, MA) for 2 minutes followed by mixing and transferring to a black 96 well assay plate ...
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bioRxiv - Immunology 2023Quote: ... Luminescence was measured after 66-72 hr of incubation using Britelite-Plus luciferase reagent (Perkin Elmer, cat. no. 6066769). Neutralization titers are the inhibitory dilution of serum samples at which relative luminescence units (RLUs ...
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bioRxiv - Bioengineering 2023Quote: ... the reagent was discarded and the tissue was imaged for bioluminescent photon emission using IVIS® Spectrum (PerkinElmer Inc.) with exposure times 1-5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620 ...
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bioRxiv - Immunology 2024Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed in TBST at least 3 times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839).
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bioRxiv - Microbiology 2019Quote: ... was achieved by addition of GDP- or UDP-GloTM nucleotide detection reagent following manufacturers’ instructions and analyzed via luminescence using a Victor 2 microplate reader (PerkinElmer).
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer). Libraries were pooled in equimolar ratios ...
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bioRxiv - Cancer Biology 2020Quote: ... amplified with RNAscope 2.5 HD Reagent Kit (Advanced Cell Diagnostics; 322300) and developed with the TSA Plus kit (PerkinElmer; NEL760001KT) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 2.7 mM KCl with 0.2% Triton X-100 and then blocked by shaking for 60 min in TSA Blocking Reagent (Perkin Elmer). Sections were left shaking for 1-3 nights at 4°C in primary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were then rinsed in TBST three times and then treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839 ...
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bioRxiv - Neuroscience 2021Quote: ... Endogenous peroxidases were blocked by treating sections with 0.3% H2O2 in methanol for 30 minutes and followed with blocking with TSA blocking reagent (Perkin Elmer) as 0.5% solution in PBS pH 7.4 for 1 h at RT ...
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bioRxiv - Genetics 2022Quote: ... the membrane was transferred to a fresh falcon tube and incubated with 1ml of Western LightningPlus-ECL chemiluminescent reagent (PerkinElmer). Proteins bands were visualised with an iBright instrument (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Cell Biology 2021Quote: ... Infection levels were inferred from the amount of luminescence in relative light units (RLUs) after adding 50 μL Britelite plus reagent (PerkinElmer) to 50 μL of media containing cell (i.e ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were washed 5 min 3 times and developed using enhanced chemiluminiscence reagent (Western Lightning Plus-ECL; Perkin Elmer). The following primary antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The tissue lysates were mixed with an equal volume of Nano-GLO Luciferase Assay Reagent (Nanolight) and luciferase activity was quantified on an Envision 2103 plate reader (PerkinElmer). Decoy concentration and half-life were determined based on a standard curve derived from the mixture of serial diluted ACE2.1mb-nLuc proteins and tissue lysate from wild-type ...
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bioRxiv - Microbiology 2022Quote: ... Quality of the libraries was measured with LabChip GX Touch HT DNA High Sensitivity Reagent Kit (PerkinElmer, Waltham, MA, USA). Sequencing was performed with NovaSeq 6000 System (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2019Quote: ... before incubated with the Fluorophore Tyramide Amplification Reagent (1:200 in amplification diluent, TSA Plus multi-fluorophore detection kit, Fluorophore: Fluorescein, PerkinElmer, NEL753001KT). Sections were washed overnight in 1x PBS at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Fifty μl of each elution fraction was suspended in 1 ml of liquid scintillation reagent (Ultima Gold, PerkinElmer, Wellesley, MA, USA), and the radioactivity in each vial was measured using a scintillation counter ...
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bioRxiv - Microbiology 2021Quote: ... a 150-μl volume of cells was resuspended and mixed by pipette with 50 μl of the luciferase substrate reagent BriteLite Plus (Perkin Elmer) in white 96-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... Final library products were assessed for quality on LabChip GX using the DNA High Sensitivity Reagent Kit (Perkin Elmer, MA, USA), diluted to 2 nM concentration and pooled ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were subsequently washed three times with TBST (15 min each) before visualization of Chemiluminescence activity using Western Lightning ECL reagent (Perkin Elmer) in Chemidoc™ MP Imaging System (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
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bioRxiv - Immunology 2020Quote: ... Results were read after 48 hours using the EnSight Multimode Plate Reader and BriteLite Plus Luciferase reagent (PerkinElmer, Waltham, MA, USA). The values were normalized ...
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bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
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bioRxiv - Neuroscience 2022Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...