Labshake search
Citations for PerkinElmer :
201 - 250 of 918 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX (Perkin Elmer, Waltham, MA). Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
-
bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
-
bioRxiv - Evolutionary Biology 2020Quote: ... DNA concentration and quality were assessed using Qubit 3.0 (Thermo Fischer Scientific, Waltham, MA) and Labchip GX Touch 24 (Perkin Elmer, Waltham, MA). Some DNA extracts that showed high levels of degradation were size-selected using Select-a-Size column or bead kits (ZYMO ...
-
bioRxiv - Systems Biology 2021Quote: ... The purity and concentration of each His6-MBP-PDZ was evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and adjusted to 4 μM by dilutions before freezing the library in 96-well plate for the holdup experiment.
-
bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
-
bioRxiv - Bioengineering 2020Quote: ... Levels of luciferase production from glioblastoma cells were measured using D-luciferin (150 μg/ml final concentration, Perkin Elmer, Waltham, MA, USA) and luminescence measurement in a plate reader after 15 minutes ...
-
bioRxiv - Cancer Biology 2020Quote: ... The molar concentration of cDNA molecules in the individual libraries was calculated from the double-stranded DNA concentration and the single library average size (determined on a PerkinElmer Labchip GX). An equal number of cDNA molecules from each library were pooled and the final pool was purified once more in order to remove any free primer and prevent index-hopping ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added as a 4x (of Final testing concentration) solution in the HEPES buffer described previously into a 384w AlphaPlate (Perkin Elmer #6005350). Then the integrin and ligand were added sequentially as 8x (4 μL each) ...
-
bioRxiv - Cell Biology 2022Quote: ... The molar concentration of cDNA molecules in the individual libraries was calculated from the double-stranded DNA concentration and the single library average size (determined on a PerkinElmer Labchip GX). An equal number of cDNA molecules from each library were pooled and the final pool was purified once more in order to remove any free primer and prevent index-hopping ...
-
bioRxiv - Microbiology 2019Quote: Methanol concentrations were monitored with a gas chromatograph equipped with a headspace sampler and a flame ionization detector (Clarus 600; PerkinElmer Inc., CA). Methane in the headspace was measured by gas chromatography with a flame ionization detector (Shimadzu ...
-
bioRxiv - Cell Biology 2019Quote: The comparative analysis of copper concentrations from each sample was carried out using an AAS equipped with a graphite furnace (PerkinElmer, AAnalyst 800). A known mass of sample was acid digested in concentrated HNO3 ...
-
bioRxiv - Biophysics 2020Quote: ... a fast and mild filtration step is performed and the tag-PDZ concentrations were measured by capillary electrophoresis instrument (LabChip GXII, PerkinElmer, Massachusets, USA). A detailed protocol of how to run the holdup assay in an automatic way using liquid handling robots can be found in [29] ...
-
bioRxiv - Cell Biology 2020Quote: ... the Mg2+ concentration in the PBS was measured by inductively coupled plasma optical emission spectrometry (ICP-OES, Optima 2100DV, Perkin Elmer, USA).
-
bioRxiv - Neuroscience 2022Quote: ... The reaction was started by adding various concentrations of GABA and 0.2 nM of [35S]GTPγS (PerkinElmer, Waltham, United States of America) in a final volume of 200 μl per point and assayed as described earlier (Rajalu et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... The K+ concentration in the xylem sap and phloem exudate were determined using inductively coupled plasma/optical emission spectrometry (PerkinElmer, Waltham, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... was added to a final concentration of 150 μL/mL and luciferase signal was measured using the Envision® 2105 Multimode Plate Reader (Perkin Elmer). Alternatively ...
-
bioRxiv - Plant Biology 2023Quote: ... Concentration of 66Zn and 67Zn were measured by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS, NexIon 300X; Perkin Elmer, Waltham, MA, USA) at isotope 66Zn (approximately 27.9% of total Zn in natural ...
-
bioRxiv - Neuroscience 2024Quote: ... and 50 µl of radioligand diluted in binding buffer ([3H]-N-methylspiperone: 0.4 nM final concentration, Perkin Elmer, Waltham, MA; [3H]-(R)-(+)-7-OH-DPAT ...
-
bioRxiv - Microbiology 2023Quote: ... The 3280 compounds were tested in a final concentration of 10 µM with the rhlA-gfp [30] reporter strain in 96 wells microtiter dish (Black Isoplate®, Perkin Elmer). The cells were added in a final concentration of OD600=0.1 and final volume of 200 µl in ABGC medium and incubated at 37°C with shaken (200 rpm) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the concentration of each sample was determined using Lunatic plates (Unchained Labs, Pleasanton, CA) read on a DropletQuant spectrophotometer (PerkinElmer, Waltham, MA).
-
bioRxiv - Cell Biology 2020Quote: ... metabolically labelled by supplementing starvation medium with35S-Met/Cys (EXPRE35S35S Protein Labelling Mix (PerkinElmer), 80 μCi/6 cm plate ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then labelled by adding the EXPRE35S35S Protein Labeling Mix (PerkinElmer EasyTagTM NEG772) at a ratio of 9 μL per mL of the concentrated culture ...
-
bioRxiv - Biophysics 2020Quote: ... Proteins were detected using enhanced chemiluminescence (Western Lightning Plus-ECL; Perkin Elmer, Waltham, MA). The antibodies used in the assay are listed in the antibody section.
-
bioRxiv - Cell Biology 2021Quote: ... Protein detection was performed using Super Signal chemiluminescent ECL-plus (PerkinElmer, Waltham, MA, USA) on a BioRad Gel doc (Bio Rad ChemiDoc Touch ...
-
bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
-
bioRxiv - Genetics 2022Quote: ... To metabolically label the cells 5uL of EasyTag EXPRESS 35S protein labeling mix (PerkinElmer), was added to 10mL of SPO cultures and incubated with shaking for 10 minutes at 30°C ...
-
bioRxiv - Cell Biology 2022Quote: LTE cell-free protein expression in combination with AlphaLISA® approach (Perkin Elmer, AUS) was performed for pairwise protein interaction analysis of PER2 and CRY2 proteins with CAVIN3 and CAVIN1 protein variants ...
-
bioRxiv - Biochemistry 2023Quote: ... Bromodomain protein was co-incubated with test compounds using 384-well in AlphaPlates (PerkinElmer) in the absence or presence of DCAF16 (1 µM ...
-
bioRxiv - Molecular Biology 2024Quote: ... proteins on PROTEAN® TGX™ gel were transferred onto nitrocellulose membranes (Perkinelmer, NBA085A001EA) with Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: Chemiluminescence detection of the proteins was performed using Western Lightning ECL-Plus (Perkin Elmer) and an Odyssey CLx device (LI-COR) ...
-
bioRxiv - Microbiology 2024Quote: ... Each collected sample was incubated with EasyTag EXPRESS-35S protein labelling mix (Perkin Elmer) at 23 µCi mL-l for 2 min at 37 °C ...
-
bioRxiv - Genetics 2023
-
bioRxiv - Cell Biology 2019Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols (Wang et al. ...
-
bioRxiv - Physiology 2020Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols(Wang et al. ...
-
bioRxiv - Biochemistry 2022Quote: ... (PerkinElmer AlphaScreen Histidine Detection Kit) were added under low light conditions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and TSA kit (Perkin Elmer) for fluorescent visualization.
-
bioRxiv - Neuroscience 2022Quote: ... or ATPlite kit (PerkinElmer, 6016941) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
-
bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
-
bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
-
bioRxiv - Biochemistry 2023Quote: ... AlphaScreen Histidine detection kit (PerkinElmer) consisting of streptavidin donor beads and nickle chelate (Ni-NTA ...
-
bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
-
bioRxiv - Cell Biology 2023Quote: ... Enhanced Chemiluminescence Substrate kit (PerkinElmer). Images were acquired using the iBright FL1000 imaging system (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2024Quote: ... (PerkinElmer AlphaScreen Histidine Detection Kit) were added under low light conditions ...
-
bioRxiv - Bioengineering 2019Quote: ... while P and K were analysed by digestion with nitric acid (HNO3) and concentrations measured with inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 800, Winlab 5.5, PerkinElmer Inc.,Waltham, MA, USA).
-
bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... each compound (as single concentration or two-fold titrations) was transferred into individual wells of a 384-well tissue culture plate (Perkin Elmer, cat 6007460) prior to addition of 120 parasites ...
-
bioRxiv - Plant Biology 2020Quote: ... concentrations were measured by Inductively Coupled Plasma Mass Spectrometry using Dynamic Reaction Cell technology (ICP-MS ELAN DRC II, PerkinElmer Inc., MA, USA) (Benedicto et al. ...
-
bioRxiv - Microbiology 2020Quote: ... at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer, Waltham, Massachusetts, United States) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... blood plasma collected from the tip of the tail vein was diluted with equal volume of PBS (pH 7.4) and analyzed for DX-4000-FITC concentration with a fluorescence spectrophotometer (HTS-7000 Plus-plate-reader; Perkin Elmer, Wellesley, Massachusetts, USA) at an excitation wavelength of 485 nm and emission wavelength of 535 nm ...