Labshake search
Citations for PerkinElmer :
501 - 550 of 568 citations for Phosphatidylinositol PI CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
-
bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
-
bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested post-stimulation and the production of TNF-α was assessed using the AlphaLISA Immunoassay Kit (PerkinElmer, AL208C) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: The AlphaLISA pSTAT1 assay was performed using the AlphaLISA SureFire Ultra p-STAT1 (Tyr701) assay kit (PerkinElmer, Inc., Waltham, MA, USA). HeLa cells (CCL-2 ...
-
bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
-
bioRxiv - Immunology 2019Quote: ... Wells were then washed twice and the number of adherent cells was determined using the ATPlite luminescence assay kit (PerkinElmer, Waltham, MA).
-
bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Detection of either ERK1/2 or Akt1/2/3 phosphorylation was performed as described in the corresponding AlphaScreen SureFire kits and fluorescence measured with an EnVision plate reader (PerkinElmer, Boston, MA). Data were normalised to the response elicited upon stimulation of cells with 10% FBS.
-
bioRxiv - Microbiology 2021Quote: ... 1-3ng/μl of the PCR amplifier of the HPV L1 gene was mixed with 8 μL of ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit version 1.1(Perkin Elmer Biosystems, USA), 2 pmol of primer ...
-
bioRxiv - Microbiology 2021Quote: ... using 100 ng total RNA for library preparation with the NEXTFLEX® Small RNA-Seq Kit v3 (PerkinElmer Inc., Waltham, MA, USA), followed by library sequencing on one lane NovaSeq6000 SP v1.0 (2×50bp) ...
-
bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
-
bioRxiv - Microbiology 2019Quote: ... bacteria DNA was extracted using the chemagic STAR DNA BTS Kit for the automated isolation of DNA (Perkin Elmer, Wellesley, MA, USA). Quality and purity of the isolated DNA were checked by spectrophotometry on the NanoDrop (Fisher Scientific ...
-
bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
-
bioRxiv - Physiology 2020Quote: Renal tissue cAMP production following ligand stimulation was measured using the time-resolved fluorescence energy transfer (TR-FRET) based LANCE ULTRA cAMP Kit (PerkinElmer, MA, USA) in combination with an EnSight Multimode Plate Reader (Perkin Elmer ...
-
bioRxiv - Neuroscience 2020Quote: ... 75 µL of supernatant from each well was collected for ATP supernatant detection using the ATPlite Luminescence Assay Kit (Perkin Elmer, 6016943) as described by the kit protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo® kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Each experiment shown is representative of three independent experiments.
-
bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
-
bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
-
An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
-
bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
-
bioRxiv - Physiology 2023Quote: ... and blood was collected for the measurement of both glucose (Accu-Check) and insulin levels (Insulin AlphaLISA detection kit, Perkin Elmer, USA).
-
bioRxiv - Physiology 2023Quote: ... Perfusates were continuously and automatically collected into 96-well plates for the detection of insulin (Insulin AlphaLISA Detection Kit, Perkin Elmer, USA). Insulin measurement was performed according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2023Quote: ... These purified ribosome footprints were used as input for the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06). The resulting cDNA was amplified by 11-16 cycles of PCR with a barcode incorporated in the primer ...
-
bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
-
bioRxiv - Cell Biology 2020Quote: LH levels in the medium were measured by means of a time-resolved fluorometric immunoassay with rat LH assay kits (National Hormone and Peptide Program, NHPP) and the Delfia system (Perkin Elmer, Waltham, MA) as reported previously (Kawaminami et al. ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
-
bioRxiv - Cell Biology 2021Quote: ... the RBP-RNA complex was purified and the bound RNA eluted for subsequent Illumina library preparation (NEXTFLEX small RNA library preparation kit v3, PerkinElmer #NOVA-5132-06) and paired-end (40×43 cycles ...
-
bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
-
bioRxiv - Biochemistry 2020Quote: The amounts of cAMP in the cytosol were measured by using the time-resolved fluorescence resonance energy transfer (TR-FRET) kit from Perkin Elmer (EnVision Xcite), according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
-
bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
-
bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays, Perkin Elmer, Codolet, France) according to the manufacturer’s recommendations (112) ...
-
bioRxiv - Developmental Biology 2022Quote: Intracellular ATP and extracellular lactate levels were measured for each NCC primary cultures after Seahorse analysis using an ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943) and the Lactate Fluorometric Assay kit from BioVision (cat ...
-
bioRxiv - Physiology 2020Quote: ... 1 ng of eluted and purified DNA was used to prepare DNA sequencing library with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, NOVA-5188-01) on the NextSeq 500 system (Illumina ...
-
bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
-
bioRxiv - Genetics 2020Quote: ... and used as input for small RNA library prep using the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific - PerkinElmer NOVA-5132-06). Libraries were prepared according to manufacturer’s instructions (V19.01) ...
-
bioRxiv - Plant Biology 2020Quote: The AlphaScreen® (Amplified Luminescent Proximity Homogeneous Assay) was carried out using an AlphaScreen® FLAG® (M2) Detection Kit (Perkin Elmer, MA) to detect protein-protein interactions ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
-
bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
-
bioRxiv - Microbiology 2021Quote: DNA library preparation was carried out according to the manufacturer’ ss protocol using the NEXTflex™ ChIP-Seq Kit (NOVA-5143-01) (PerkinElmer, Hamburg, Germany) and the NEXTflex™ ChIP-Seq Barcodes (NOVA-514122 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Extracted DNA was sent to Texas A&M Agrilife Bioinformatics and Genomics Service (College Station, TX, USA) for whole-genome library preparation using a NEXTFLEX Rapid XP DNA-Seq Kit HT (PerkinElmer, Waltham, MA, USA). Libraries were sequenced at the Texas A&M Agrilife Bioinformatics and Genomics Service on one NovaSeq 6000 S4 lane at 26.1 ± 5.8 depth of coverage per sample across the full genome (2 s.d. ...
-
bioRxiv - Microbiology 2022Quote: Quality and quantity of the extracted RNA samples were analyzed with LabChip GX Touch HT RNA Assay Reagent Kit (PerkinElmer, Waltham, MA, USA) and Qubit RNA BR kit (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN) of 9.5 using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit (PerkinElmer®, Waltham, MA). Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...
-
bioRxiv - Genomics 2022Quote: ... 1 ng of eluted and purified DNA was used to prepare libraries with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, #NOVA-5188-01) on the Illumina NextSeq500 system using a single read 100 bp protocol ...
-
bioRxiv - Biochemistry 2023Quote: The expression of IL-6 and TNF-α in culture supernatants was detected using the corresponding AlphaLISA Immunoassay Kit (PerkinElmer, AL223C and AL208C) according to the manufacturer’s protocol.
-
bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...