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Citations for PerkinElmer :
151 - 199 of 199 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and seeded at 0.6 × 106 cells/ml on a poly-lysine/ laminin substrate in a 96 well plate (PerkinElmer #6005558). Primary cortical neurons were cultured for 5 days and then transfected using Lipofectamine 2000 (ThermoFisher # 11668019) ...
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bioRxiv - Neuroscience 2020Quote: To assess the functionality of the efflux transporter P-gp the Papp of the radiolabeled P-gp substrate [3H]-digoxin (PerkinElmer) was measured in the luminal to abluminal (L-A ...
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bioRxiv - Genetics 2019Quote: ... PRMT7 wild-type and mutants were incubated with putative substrates in PBS with 2 μCi of S-adenosyl-[methyl-3H]methionine ([3H]AdoMet) (55-85 Ci/mmol, PerkinElmer) for 18 h at 26°C ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were injected intra-peritoneally with XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate (15 mg/ml, 100ul/10g; Perkin Elmer). The mice were imaged with Xenogen IVUS Spectrum (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured after 40 h of incubation by addition of an equal volume of substrate NeoLite (Perkin Elmer) followed by luminescence read out on the EnSight Multimode Plate Reader (Perkin Elmer) ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Microbiology 2023Quote: ... the free fusion protein was removed by washing with PBS and cell-bound luciferase activity was measured using NanoGlo luciferase substrate (Nanolight) in an Envision 2103 microplate luminometer (PerkinElmer) (Tada et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Immunology 2024Quote: ... Tumor engraftment was confirmed on day 6 via intraperitoneal injection of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (XenoLight, PerkinElmer), followed by bioluminescent imaging (BLI ...
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bioRxiv - Biophysics 2023Quote: ... Leucine affinities for WT LeuT and mutants displaying WT substrate affinities (A22V, A22S) were determined by saturation binding of [3H]leucine (25 Ci/mmol) (PerkinElmer), with unspecific binding corrected for by the addition of 100 µM unlabelled leucine ...
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bioRxiv - Microbiology 2024Quote: Activities of NpaA1 and AtdA1 against different substrates were analyzed spectrophotometrically with a Lambda 25 spectrophotometer (PerkinElmer/Cetus, Norwalk, CT) by following the disappearance of tested substrates at individual defined wavelengths ...
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bioRxiv - Immunology 2024Quote: ... to measure bioluminescent signal after intraperitoneal administration of 0.15 mg of luciferin substrate per gram of body weight (PerkinElmer 122799). Total photon counts were quantified using LivingImage software ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... a 150-μl volume of cells was resuspended and mixed by pipette with 50 μl of the luciferase substrate reagent BriteLite Plus (Perkin Elmer) in white 96-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... An equal volume of cell culture media and esterase substrate (100 μM in esterase assay media at pH 5) was transferred to a black walled clear bottomed plate (Perkin Elmer) and fluorescence was measured after 1 h.
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bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The 500 nM annealed DNA substrates were then radiolabeled in PNK buffer with PNK enzyme and radioactive ATP [γ32P] from Perkin Elmer at 37°C for 1 hr then diluted in TE buffer (10 mM Tris ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Luminescent signals were generated after incubation with SuperSignal® West Pico Chemiluminescent substrate (Pierce Biotechnology) and captured with Kodak Biomax X-ray film (PerkinElmer).
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bioRxiv - Cancer Biology 2022Quote: ... Imaging of grafted tumors was performed using an IVIS in vivo imager (LAGO) after intraperitoneal injection of RediJect D-Luciferin Bioluminescent Substrate (PerkinElmer, #770504) 13 weeks after injection ...
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bioRxiv - Cancer Biology 2022Quote: ... Imaging of grafted tumors was performed monthly using an IVIS in vivo imager (LAGO) after intraperitoneal injection of RediJect D-Luciferin Bioluminescent Substrate (PerkinElmer, #770504).
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bioRxiv - Cancer Biology 2023Quote: ... Mice were anesthetized at 37 °C with inhaled isoflurane in an induction chamber for 5 min and received an intraperitoneal injection of luciferin (150 mL, RediJect D-Luciferin Ultra Bioluminescent Substrate, PerkinElmer, 770505). Bioluminescence images were taken 13 minutes after injection ...
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bioRxiv - Neuroscience 2023Quote: ... 50 μl of media from each sample and 50 μl of the enzyme and substrate mix was pipetted in a well of the imaging plate (PerkinElmer, 6055300). The plate was briefly mixed and incubated for 1 h at RT avoiding exposure to light ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... with the luciferase substrate D-luciferin (200µL of 15mg/ml - assuming an average animal weight of 24-26gr) (Perkin Elmer, 122799) and incubated for 10 minutes at room temperature before images were taken using IVIS Spectrum In Vivo Imaging System (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were diluted 1:5000 in TBS-T with 5% milk and incubated with the membrane at room temperature for an hour before performing the chemiluminescence reaction (Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate, Perkin Elmer NEL105001EA). Signal was captured with either X-ray films (CL-XPosure™ Film ...
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bioRxiv - Immunology 2022Quote: ... luciferase activity was quantified by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (PerkinElmer, Beaconsfield, UK). Samples which reduced the infectivity of the pseudotypes by at least 90% were classed as positive ...
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bioRxiv - Physiology 2023Quote: ... Elastase activity measurements in whole aorta tissue or tissue sections were performed using the far-red fluorescent elastase substrate Neutrophil Elastase 680 FAST probe (NE680; Perkin Elmer # NEV11169). Immunofluorescent detection of neutrophil elastase was performed on aortic sections from apoE-/- mice (The Jackson Laboratory strain # 002052) ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Physiology 2023Quote: ... was prepared by mixing 40 µL homogenate with an equal volume Nanoluc reaction buffer (PBS: NanoGlo Buffer: NanoGlo substrate furimazine, 30: 10: 0.2) in a black perkin elmer plate (6065400, PerkinElmer, Waltham, MA, USA). Within 2 min of buffer addition ...
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bioRxiv - Immunology 2023Quote: ... 50 µL of culture medium was removed from the plates and 30 µL of luciferase substrate (Perkin Elmer Britelite Plus Catalog #6066769) was added ...
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bioRxiv - Biophysics 2024Quote: The amount of 32P incorporated into the Abl substrate was quantified using a liquid scintillation counter (PerkinElmer, model: Tri-Carb®4910TR). The Michaelis-Menten parameters and their errors were obtained by Monte Carlo fitting of the sample mean and standard error of the mean using NumPy (Harris et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Immunology 2021Quote: ... luciferase activity was quantified by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK).
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bioRxiv - Physiology 2022Quote: ... The assay was a mobility shift assay based on charge differences before and after electrophoretic separation of product from fluorescently labeled substrate read using a Caliper EZ Reader (Perkin Elmer, Boston, MA). All reactions took place in the presence of excess nicotinamide adenine dinucleotide and the known SIRT1 inhibitor suramin was used as a control.
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bioRxiv - Neuroscience 2022Quote: ... or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... while the activity of the radiolabeled substrate was measured by liquid scintillation counting (Perkin Elmer MicroBeta2 liquid scintillation counter, Perkin Elmer, Waltham MA). ABC transporter-related vesicular transport was calculated by subtracting passive uptake measured without Mg-ATP (or in the presence of Mg-AMP ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then washed twice with PBS/0.05%Tween and once with PBS and finally the signals were detected using Western Lightening chemiluminescent substrate (Perkin Elmer, Netherlands, Cat #NEL104001EA).
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bioRxiv - Immunology 2023Quote: ... luciferase activity was quantified by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK). Antibody titre was then estimated by interpolating the point at which infectivity had been reduced to 50% of the value for the no serum control samples.
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was quantified after 48–72 h by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK). Antibody titre was then estimated by interpolating the point at which infectivity had been reduced to 50% of the value for the ‘no serum’ control samples.
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bioRxiv - Biochemistry 2019Quote: ... Visualization of EOI was performed with enhanced chemiluminescent reagent (Pierce ECL Western Blotting Substrate) using an IVIS®Spectrum imaging system (Perkin Elmer, Hopkinton, MA) with blocked excitation filter and opened emission filter ...