Labshake search
Citations for PerkinElmer :
101 - 136 of 136 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP enzyme activity was determined after addition of 35 µl per well of a 1:1 mix of Western Lightning oxidizing and luminal reagents (Perkin Elmer Life Sciences) supplemented with 150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... HRP enzyme activity was determined after the addition of 40 μL of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Biochemistry 2020Quote: ... Quantitative PCR (qPCR) was performed on an ABI 7900HT sequence detection system (Perkin Elmer, Applied Biosystems) using TaqMan™ Gene Expression Assays (FAM-labelled ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Semi-quantitative RT-PCR was carried out in the presence of [α-32P] dCTP (Perkin Elmer) with primer sequences provided in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Microbiology 2019Quote: ... the cells were starved for 30 min in the methionine-free medium before pulse-labeling with 25 µCi/ml of Easy Tag Express 35S labeling mix (Perkin Elmer, Shelton, CT, USA) for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning ECL reagents (Perkin Elmer Life Sciences, Waltham, MA, USA). Light emission was measured with an LB 941 TriStar luminometer (Berthold Technologies ...
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bioRxiv - Physiology 2023Quote: ... the medium was exchanged for DMEM medium supplemented with emetine and 35S-Protein Labelling Mix (35S-Met and 35S-Cys, Perkin Elmer NEG072; 100 µCi/mL). Cells were incubated for 3 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences, Waltham, MA, USA), quantification of bands was performed using the ImageLab software (Bio-Rad).
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were cleaned with the Biomek FX robot using the chemagic SEQ Pure20 Kit (PerkinElmer, CMG-458). Clean PCR amplicons from the same DNA sample were pooled to generate 137 libraries that were sequenced by Illumina MiSeq v3 ...
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bioRxiv - Plant Biology 2023Quote: ... The size and abundance of the resultant PCR products were then analyzed and quantified by LabChip GX bioanalyzer (PerkinElmer). The expression analyses represent two to three biological replicates ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... 130 of the colonies were analysed by colony PCR (using an M13 primer set, Table S1) and capillary electrophoresis (LabChip GX, PerkinElmer) using the DNA 1K Reagent Kit with DNA HT 5K LabChip single sipper chip ...
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... Probes were prepared by random priming of corresponding PCR products (5’ and GFP templates) in the presence of [αP32]CTP (PerkinElmer BLU513H100UC). Unincorporated nucleotides were removed using a Sephadex G-50 column (GE Healthcare 28-9034-08) ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 120 ng PCR product was mixed with 7.5 mM ribonucleotide solution (ATP, GTP, UTP, CTP) and 133 nM α-32P-ATP (Perkin Elmer) as a tracer ...
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bioRxiv - Immunology 2022Quote: ... The PCR products were analyzed by heteroduplex mobility assay using microfluidic capillary electrophoresis system caliper LabChip GX (PerkinElmer, Villebon-sur-Yvette, France). The homozygous Il34-/- specimen were identified from Il34+/+ littermate as previously described (13).
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bioRxiv - Molecular Biology 2023Quote: ... were prepared in in vitro transcription reaction on linear PCR-generated template containing RNAImod (4) with 500 μM ATP or NAD and 25 μM [α 32P] CTP (Perkin Elmer) using hexa-histidine tagged E ...
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bioRxiv - Molecular Biology 2019Quote: ... the templates of dsRNA were amplified from the above pBluescript KS (+) vectors inserted diap1 sequences in each insect by PCR using AmpliTaq Gold (Perkin Elmer, Boston, USA). The PCR primers are described in Table S8 (T7-KS/T7-SK) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were added to the PCR product and sequencing was conducted using the automated DNA Sequencer (ABI 3130xl Genetic Analyzer; Perkin Elmer/Applied Biosystems). Using the software Genemapper ver ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Molecular Biology 2020Quote: ... Double-stranded DNA probes were synthesized by random-prime labeling of PCR fragments with [α-32P]dATP and [α-32P]dTTP (PerkinElmer, Waltham, MA). Probe locations are as follows with numbering relative to the start codon of the indicated protein-coding gene ...