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Citations for PerkinElmer :
701 - 728 of 728 citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... pseudoviruses were added in quadruplicate on HEK293T-ACE2 target cells which were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer, Waltham, MA, USA) 24h before infection ...
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bioRxiv - Cancer Biology 2024Quote: ... then separated in an 1% formaldehyde-containing agarose gel and transferred onto a GeneScreen Plus hybridization transfer membrane (Perkin Elmer, Waltham, MA, #NEF987001PK). RNAs on the membrane were crosslinked by exposing to UV light ...
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bioRxiv - Immunology 2021Quote: ... Plasma 2-[1-14C]-Deoxy-D-glucose radioactivity was directly measured in a liquid scintillation counter (Tri-Carb 2810 TR, Perkin Elmer, Waltham, MA, USA). Tissue lysates were processed through ion exchange chromatography columns (Bio Rad Laboratories Inc. ...
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bioRxiv - Evolutionary Biology 2019Quote: ... followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents, Perkin Elmer TSA Plus Cy3 or Cy5 Kit). Residual enzyme activity was inhibited via 45-minute incubation in 0.1% hydrogen peroxide in PTW followed by four PTW washes prior to addition and development of the second peroxidase-conjugated antibody76.
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bioRxiv - Microbiology 2021Quote: ... in a ratio of 5:95 and analyzed at the mid-IR region (400-4,000 cm-1) of the spectrometer (Perkin Elmer Spectrum 100, United States). The biodegraded products of AB1 were identified using LC-MS analyses (Dionex Ultimate 3000 ...
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at room temperature with alkaline phosphatase-coupled goat antirabbit antibodies and developed with Western Lightning Plus (Perkin Elmer, Inc., Waltham, MA) according to manufacturer directions.
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Pelleted particles were resuspended in D10 medium and the concentration of HIV p24 was quantitated by using an HIV-1 p24 ELISA Kit (Perkin Elmer, Boston, MA, USA) [44 ...
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bioRxiv - Cell Biology 2023Quote: ... Spectrum Spotlight 400 FT-IR Imaging system was used to acquire spatial resolution images in the mid-IR region from 4000 to 800LJcm−1 (Perkin Elmer, Waltham, MA, USA) of three consecutive sections/disc to minimize section-based variation ...
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bioRxiv - Cancer Biology 2024Quote: ... The tumor burden was quantified weekly by bioluminescent signal intensity on isoflurane-anesthetized mice that received a subcutaneous injection of D-luciferin (150 μg luciferin/1 g mouse body weight) 10 min prior to IVIS® imaging (PerkinElmer, Waltham, MA,USA). Survival data were presented and reported in Kaplan–Meier plots.39
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...
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bioRxiv - Biophysics 2019Quote: ... The solubilized virus was diluted in HB buffer with 1% Triton-X and the fluorescence emission spectrum of the sample was measured using a fluorimeter (Perkin Elmer LS 55, Waltham, MA). The integrated fluorescence intensity across the spectrum was calculated and compared to a standard curve of known dye concentration in the same buffer to determine the concentration of fluorescent dye in the viral suspension ...
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bioRxiv - Genetics 2019Quote: ... were mounted on infrared windows where spectral images were collected at a 4 cm-1 spectral resolution and ~7 μm spatial resolution from a Spotlight 400 Imaging system (Perkin Elmer Instruments, Shelton, CT USA). Background spectra were collected under identical conditions from clear Ba2F windows and subtracted from sample data by instrumental software ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse Chd7 primary antibody was followed by signal amplification with goat anti-rabbit IgG horseradish peroxidase (1: 500) (Perkin Elmer Inc: NEF812001EA, Waltham, MA). The tyramide signal amplification plus Cy3 Kit (1:1500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
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bioRxiv - Developmental Biology 2024Quote: ... The heads were rinsed five times in PBST at room temperature and then incubated in 50x diluted Cy3 Tyramide Reagent (PerkinElmer Life Science, Inc. 1:50 dilution) in Amplification Dilution buffer for 90 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired for 60 min at 1 frame/15-30 s using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective). Images were analyzed using Volocity Suite (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... collected water samples (45 ml in 50 ml Falcon tubes, with small aerobic headspace) were spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h at ∼ 20 °C in the dark ...