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Citations for PerkinElmer :
51 - 100 of 1167 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Ligand-induced ERK1/2 phosphorylation was assessed using the AlphaLISA Surefire Ultra p-ERK1/2 (Thr202/Tyr204) kit (PerkinElmer, Melbourne, Australia) following the manufacturer’s protocol as previously described (23 ...
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bioRxiv - Immunology 2022Quote: ... Levels of p24 antigen in supernatant were quantified by p24 ELISA (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and an ELAST enzyme-linked immunosorbent assay (ELISA) amplification system (PerkinElmer, Inc.) was also used ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatants were collected for ELISA and D-luciferin (122799, PerkinElmer, 150ug/mL) was added to the wells and incubated for 5 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: AlphaLISA® detection kit targeted against IAV NP protein was purchased from Perkin Elmer and performed according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and developed for 2 minutes using Tyramide Signal Amplification (TSA Plus kit, PerkinElmer). We stained for emphTg(isl1:GFP ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Immunology 2022Quote: ... Viral stocks were quantified for viral CAp24 content by ELISA (Perkin Elmer, #NEK050A) and by qPCR quantification of lentiviral integrated copies in human genome of MDMs as previously described34,37 ...
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bioRxiv - Microbiology 2021Quote: ... The p24gag content of all viruses was quantified using an ELISA (Perkin Elmer) and viruses were titrated in HEK-293T overexpressing the human ACE2.
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bioRxiv - Microbiology 2022Quote: ... The p24gag content of all viruses was quantified using an ELISA (Perkin Elmer) and viruses were titrated in HEK-293T overexpressing the human ACE2.
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bioRxiv - Microbiology 2023Quote: ... Viral stocks were titrated using HIV-1 P24 ELISA assay (#NEK05001KT, Perkin Elmer) and the concentration was 200 ng/ml.
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bioRxiv - Immunology 2024Quote: ... The target proteins were visualized by Western Lightning PLUS-ECL or Ultra kit (Perkin Elmer). The images were acquired by Azure Biosystems C300 or Thermo Fisher Scientific iBright FL1000 ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) and by real time PCR ...
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bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) or by qPCR ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and protein expression signals were detected using a chemiluminescence detection kit (Perkin Elmer, Waltham, MA, USA) and visualised using a chemiluminescence imaging system (Fusion Solo S ...
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bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Immunology 2019Quote: ... 14 and 21 for HIV-1 p24 antigen levels by ELISA (Perkin Elmer, Boston, MA).
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Microbiology 2020Quote: ... ELISA absorbance was read at 450nm for 0.1 second on a Victor X5 plate reader (PerkinElmer).
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bioRxiv - Cell Biology 2019Quote: ... ELISA plates were read at 450 nm using a Wallac 1420 Victor2 Microplate Reader (Perkin Elmer, provided by Don Jarvis at the University of Wyoming) ...
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bioRxiv - Microbiology 2023Quote: ... 0.45 µm-filtered and used for HIV-1 CAp24 quantification by ELISA (released CAp24) (Perkin Elmer). Cell lysates were analyzed by western blotting.
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bioRxiv - Biochemistry 2020Quote: ... 50 µg/mL PEG20,000 (SERVA; Cat. # 33138), ATP (Sigma; Cat. # A-7699) (variable, corresponding to ATP-Km of the respective kinase, [33P]- γ-ATP (PerkinElmer; Cat. # NEG302H): approx ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Biochemistry 2023Quote: ... 9-1-1 having an N-terminal 7-residue kinase recognition sequence on Mec3 was radiolabeled to a specific activity of approximately 70 cpm/fmol with [γ-32P] ATP (PerkinElmer Life Sciences, Inc) using the recombinant catalytic subunit of cAMP-dependent protein kinase produced in E ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... with HRP goat anti-mouse IgG Ab (31430, 1:20,000) for 1 h and developed using a Western Lightening Enhanced Chemiluminescence kit (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2019Quote: ... loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit; PerkinElmer). For Flag immunoprecipitations ...
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bioRxiv - Molecular Biology 2021Quote: ... Levels of HIV-1 gag p24 in culture supernatants were measured by ELISA as instructed by manufacturer (PerkinElmer).
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bioRxiv - Neuroscience 2019Quote: ... The optical density was measured by an ELISA reader (EnSpire, Multimode Plate Reader, PerkinElmer do Brasil, SP, Brasil).
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bioRxiv - Cell Biology 2019Quote: ... p-ERK detection was performed using the AlphaLISA SureFire Ultra p-ERK 1/2 (Thr202/Tyr204) Assay Kit (PerkinElmer) per the manufacturer’s specifications in 1/2-area white 96-well plates ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Animals were injected intravenously 24h after the last pharmacological treatment with Hypoxisense 680 (2 nmol/100 uL per mouse, Perkin Elmer, Life Sciences, Zaventem, Belgium), and the Hypoxisense 680 was measured in vivo 24h post-injection using the IVIS Spectrum (Perkin Elmer ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...