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Citations for PerkinElmer :
351 - 400 of 479 citations for Mouse Anti Herpes Simplex Virus 2 gD 0192 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured for 90 minutes at 2-min intervals using a 340/460 excitation/emission filters on a Envision plate reader (Perkin Elmer). The IC50 values were calculated by fitting the curves using a four-parameter equation in GraphPad Prism ...
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bioRxiv - Microbiology 2022Quote: ... Cells (2 × 104 cells/well) were seeded into 96-well plates (Cell Carrier-96well Ultra microplate; PerkinElmer Inc., Waltham, MA, USA) and differentiated to macrophages with phorbol 12-myristate 13-acetate (10 ng/mL ...
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bioRxiv - Biochemistry 2022Quote: ... A competitor unlabelled DNA of the same sequence was added at 2 µM and FP was measured over time on a VictorV plate reader (Perkin Elmer). Experiments with PARP1 were carried out in the same conditions as for PARP2 with the exception that the reaction buffer which was 12 mM Hepes pH 8.0 ...
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bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
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bioRxiv - Synthetic Biology 2022Quote: ... Mice were monitored for disease progression 1-2 times per week via bioluminescence imaging (BLI) using an IVIS imaging system (Perkin Elmer). For CHLA-255 models ...
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bioRxiv - Microbiology 2022Quote: ... 2 x 105 parasites were applied to each well of a clear-bottomed 96-well plate (Perkin Elmer cat. no. 6055300) seeded with HFF cells ...
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bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Physiology 2023Quote: ... the animals were fasted overnight and a bolus of 2-deoxy-D-[1,2-3H]-glucose (1mC/ml; specific activity: 20Ci/mmol; PerkinElmer, Madrid, Spain) mixed with glucose (100µCi/kg body weight ...
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bioRxiv - Molecular Biology 2023Quote: ... the reaction was initiated by the addition of 3 μl of 2 mM [ψ-32P]ATP (222 TBq/mmol Perkin Elmer) to the mixture (final concentration ...
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bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Microbiology 2024Quote: ... 2×106 bacteria/mL in PBS were seeded in 384-well black clear-bottom plate (ViewPlate®-384 F TC, PerkinElmer) in the presence of 12.5 % BRC ...
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bioRxiv - Neuroscience 2024Quote: ... and counted for 2 min after a 4 hr delay on a Perkin Elmer Betaplate 1205 liquid scintillation counter (Perkin Elmer). Nonspecific binding was determined with 1.0 µM dihydroergotamine.
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bioRxiv - Cancer Biology 2020Quote: ... together with 10 μL of Anti-rabbit IgG (AL104C, Perkin Elmer) and AlphaLISA Acceptor Beads (10 μg/mL final concentration ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP-labeled goat anti-rabbit antibody (1:500, PerkinElmer, NEF812001EA) for secondary detection ...
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bioRxiv - Plant Biology 2023Quote: ... The secondary antibody (Anti-Rabbit IgG [Goat], HRP-Labeled, PerkinElmer NEF812001EA) was 1:10000 in 5% TBST-milk.
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bioRxiv - Developmental Biology 2023Quote: ... and anti-DNP HRP-conjugated antibody (Perkin Elmer, Cat# FP 1129) followed by tyramide signal amplification (Akoya Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... HRP labelled goat anti-rabbit IgG (Perkin Elmer: NEF812001EA, 1:10,000), StarBright Blue 520 goat anti-mouse IgG (Bio-Rad 64456855 ...
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bioRxiv - Bioengineering 2020Quote: ... In vivo and ex vivo fluorescence imaging was carried out using a planar (2-D) IVIS Spectrum device (PerkinElmer, Waltham, MA, USA). Pymol (1.7 ...
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bioRxiv - Neuroscience 2020Quote: ... were harvested in Schneider’s medium on ice and transferred to Schneider’s medium containing 35S-methionine/cysteine (2 µCi/µl; Perkin Elmer Labs Inc., NEG709A) and 30 µM chloroquine (to inhibit autophagy) ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of each phase were collected and measured in a 2480 automatic Gamma counter Wizard 2 3” (PerkinElmer, Waltham, MA, USA). LogD value were calculated using Excel by dividing measured values of octanol by water and logarithmize the result ...
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bioRxiv - Microbiology 2021Quote: In all our experiments photon emission of the luminescent bacteria was recorded at regular intervals under general anesthesia (isoflurane, inspired fraction=2%) using IVIS Spectrum and analyzed with Living Image software (PerkinElmer, Richmond, CA) as previously described (Debarbieux et al. ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Luciferase activity was measured for 2 seconds in intact living cells using a microBeta Wallac luminometer (Perkin Elmer, Villebon sur Yvette, France). Tests were performed in quadruplicate in at least three independent experiments and data were expressed as mean ± s.e.m ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The OptiPlate-384 plate was incubated at room temperature in a Biosolutions AmbiStore™ D (HighRes) for 2 hours before the HTRF signal was measured on an EnVision® HTS plate reader (PerkinElmer). For OSM-induced CCL2 assay in hit validation step ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were incubated with reaction time points for 2 hours at room temperature in 384 well white microplates (PerkinElmer OptiPlate-384) then TR-FRET emission at 665 nm and 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were shaking at 999 rpm and 28°C and growth was measured by monitoring OD600 every 2 h for 10 h using a plate reader (2300 EnSpire™, Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... and the amount of 2-NBDG was measured by determining the fluorescence intensities at Ex/Em = 475/550 nm using an ARVO X2 (PerkinElmer Co., Ltd.). To investigate the effect of β-hydroxybutyrate on glucose uptake ...
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bioRxiv - Biochemistry 2024Quote: ... whereafter lysed cells were added to 2 mL scintillation fluid and radioactivity was measured using a scintillation counter (Perkin Elmer 2450 Microbeta2). Data were analyzed using GraphPad Prism 8.0.0 and fitted to a four-parameter logistic curve from where the IC50 and the 95% confidence interval were calculated ...
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bioRxiv - Physiology 2024Quote: ... Measurement of radiolabeled 2-deoxyglucose was carried out in a Tri-Carb 4910 TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA) by adding 200 μL of muscle lysate to 4 mL scintillation fluid (Perkin Elmer ...
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bioRxiv - Developmental Biology 2024Quote: ... the University of Liverpool’s centre of preclinical imaging provided the imaging services using the Quantum GX-2 system (PerkinElmer, Inc. Waltham, MA). Whole-body scan images were acquired with the protocol FOV72 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tumor burden was quantified weekly by bioluminescent signal intensity on isoflurane-anesthetized mice that received a subcutaneous injection of D-luciferin (150 μg luciferin/1 g mouse body weight) 10 min prior to IVIS® imaging (PerkinElmer, Waltham, MA,USA). Survival data were presented and reported in Kaplan–Meier plots.39
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bioRxiv - Cancer Biology 2019Quote: Sections were sequentially stained using the OPAL polymer anti-rabbit HRP (PerkinElmer) or ImmPRESS™ HRP goat anti-rat IgG polymer (MP-7404 ...
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bioRxiv - Biochemistry 2021Quote: ... 0.4 μg of the Anti-Myc coated Acceptor Beads (PerkinElmer, MA, USA) was added in 12.5 μl reaction buffer B (25 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... purified anti-IFNγ and BsAb were radiolabeled with 125I-radionuclide (Perkin Elmer) using Iodogen reaction and then purified by P-6 spin column (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with an anti-DNP-AP antibody (PerkinElmer) (1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... the secondary anti-murine IgG conjugated with horseradish peroxidase (HRP, PerkinElmer, #NEF822001EA) (1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence Imaging and Quantification: Mice were anesthetized with isoflurane (2-3% in 100% oxygen) and bioluminescence images acquired with an IVIS Spectrum (Perkin Elmer, Waltham, MA) 5 minutes after intraperitoneal injection of Potassium D-Luciferin solution (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
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bioRxiv - Physiology 2021Quote: ... were plated per well in 96-well white microplates and 100 nM of GnRH (or vehicle) were injected using Synergy 2 Multi-Mode Microplate Reader (Bio Tek) or Victor X light plate reader (Perkin Elmer Life Sciences). Luminescence signals were recorded for 30 seconds ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Microbiology 2019Quote: ... At 6 hpi the cells were starved in a glucose-free medium for 30 min followed by labeling with 200 µCi/ml of mannose-D-[2-3H(N)] (Perkin Elmer, Shelton, CT) for 1.5 h ...