Labshake search
Citations for PerkinElmer :
501 - 550 of 1184 citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
-
bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensities in the mouse dorsal skins were measured by an in vivo imaging system (IVIS Lumina XRMS Series III, PerkinElmer). The organs and tissues ...
-
bioRxiv - Cancer Biology 2023Quote: In ADM inhibition experiments wild type mouse organoids were imaged live after overnight acclimation using the Operetta High Throughput Screen imaging system (PerkinElmer) using 20× objective ...
-
bioRxiv - Immunology 2023Quote: ... Tumor growth in live mouse was monitored every 5 days by bioluminescence imaging (BLI) using IVIS Spectrum In Vivo Imaging System (PerkinElmer) after 10mins intraperitoneally injection of 150mg/Kg D-luciferin ...
-
bioRxiv - Bioengineering 2023Quote: ... tumor engraftment in each mouse was quantified via bioluminescent imaging using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer) and Living Image Software ...
-
bioRxiv - Developmental Biology 2021Quote: ... and developed for 10 minutes with fluorescein- or Cy3-conjugated Tyramide amplication system (Perkin Elmer, Shelton, CT). Amplified staining for Aggrecan was performed without detergent (1X PBS only ...
-
bioRxiv - Biochemistry 2022Quote: ... ATP concentration was varied from 0 to 1000 mM in presence of 10 nM [3H]ATP (PerkinElmer). The reaction was initiated by adding ATP to the reaction solution containing 0.005 mg/ml Cya ...
-
bioRxiv - Neuroscience 2021Quote: ... and the conjugate was detected using the Fluorescein TSA Plus System (1:10, PerkinElmer, Waltham, MA, USA) for 2 h ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
-
bioRxiv - Microbiology 2020Quote: ... and 2.5 μCi of [γ32P]-ATP (stock of 6000 Ci/mmol 10 mCi/mL, from Perkin Elmer). Autophosphorylation reactions were performed at 30 °C ...
-
bioRxiv - Physiology 2022Quote: ... and 0.01% Triton X-100) in the presence of 10 μCi [32P]-γ-ATP (Perkin Elmer Company) with a final volume of 20 μl ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Images were acquired within 10 minutes following intraperitoneal injection of 150mg/kg of D-luciferin (PerkinElmer #122799)
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to cells at a final concentration of 10 μM using a Janus Automated Workstation (PerkinElmer) equipped with a pin tool (V&P Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... Bioluminescence imaging was performed after 10-20 minutes using an IVIS Lumina II or Spectrum system (PerkinElmer). Image acquisition settings were adjusted dependent on signal saturation (exposure time ...
-
bioRxiv - Cancer Biology 2023Quote: ... by intraperitoneal injection and then imaged 10 min later using an IVIS™ Spectrum imaging system (PerkinElmer). Body weight and tumor volume were measured 1-2x weekly.
-
bioRxiv - Neuroscience 2023Quote: ... 7 increasing concentrations (0.15 – 10 nM) of [3H]-SCH23390 (specific activity 83.6Ci/mmol, Perkin Elmer, #NET930025UC, Canada) for D1 binding to generate saturation curves ...
-
bioRxiv - Bioengineering 2023Quote: ... for 10 minutes and then imaged using the In Vivo Imaging System (IVIS) Spectrum imaging system (PerkinElmer) and Living Image software (PerkinElmer).
-
bioRxiv - Immunology 2023Quote: ... 10 µl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration ...
-
bioRxiv - Cancer Biology 2021Quote: The intrinsic and extrinsic fluorescence emission spectra of the proteins were measured using an LS55 spectrofluorometer (Perkin Elmer, Waltham MA, USA). WT or N16D HsTPI (0.5 mg·mL-1 ...
-
bioRxiv - Cell Biology 2020Quote: ... the PVDF membrane was washed with TBST and incubated with appropriate secondary antibodies (horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG), followed by enhanced chemiluminescence (ECL; Visual protein, Taipei, Taiwan) and the membrane was examined by ECL spectrophotometry (Perkin Elmer, Geliance 600 Camera Imaging system ...
-
bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
-
bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
-
bioRxiv - Biochemistry 2020Quote: ... The membrane was transferred to a tube and the protein-bound [α-32P]dCTP was quantified in a Beckman Coulter LS 6500 Liquid Scintillation Counter using OptiPhase HiSafe 3 (PerkinElmer) as solvent.
-
bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
-
bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
-
bioRxiv - Microbiology 2020Quote: ... and adhesin were optimized by finding the hook point for the interaction by setting up a matrix of proteins in 96 well AlphaPlates (Perkin Elmer) in 10 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
-
bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
-
bioRxiv - Biochemistry 2020Quote: ... with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol) from Perkin Elmer. Protein Assay (Bio-Rad ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
-
bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
-
bioRxiv - Physiology 2020Quote: ... glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) knock out mice were orally gavaged with 100 μCi of [9,10-3H(N)]-Triolein (Perkin Elmer, NET431001MC) suspended in olive oil ...
-
bioRxiv - Immunology 2021Quote: ... The radioactive counts (cpms) of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer). Antibody index for each sample was calculated as follows ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
-
bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
-
bioRxiv - Cell Biology 2020Quote: ... or Caliper LabChip GX DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries were quantified using the Quant-iT PicoGreen ds DNA assay (Life Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Bands were detected with enhanced chemiluminescence (ECL) kit (Perkin Elmer).
-
bioRxiv - Developmental Biology 2020Quote: ... The TSA plus Cy3 Kit (Perkin Elmer Inc, Waltham, MA) was used for probe detection ...
-
bioRxiv - Developmental Biology 2021Quote: ... and visualized with the Tyramide Signal Amplification Kit (Perkin Elmer).
-
bioRxiv - Neuroscience 2021Quote: ... fluorescein was deposited using TSA Plus Fluorescein Kit (Perkin Elmer). To immunohistochemically visualise CRH ...
-
bioRxiv - Neuroscience 2021Quote: ... and then Tyramide Signal Amplification (TSA) Systems kit (Perkin Elmer) was used according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... The Western Lightning ECL Pro kit (Perkin Elmer, Waltham MA) was used to visualize protein bands ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... samples were incubated in Cy3 (NEL744001KT, TSA Plus Kit, PerkinElmer). To stop the POD activity ...
-
bioRxiv - Neuroscience 2020Quote: ... Cellular ATP measurements are undertaken using ATPLite kit (Perkin Elmer) as per manufacturer’s instructions ...