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Citations for PerkinElmer :
501 - 550 of 651 citations for Monocyclohexyl Phthalate Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and maintained in 2.5 mL of KSFM supplemented with 5% (v/v) charcoal-stripped FBS and 50 nM R1881 (PerkinElmer) 25 ...
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bioRxiv - Cell Biology 2022Quote: hTert-RPE1 Ruby-PCNA or hTert-RPE1 Ruby-PCNA p21-GFP H3.1-iRFP cells were seeded at a density of 2.5×104 cells/ml in 20 μl of media/well in 384-well CellCarrier Ultra plates (PerkinElmer) or 8-well Ibidi chambers one day before adding inhibitors ...
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bioRxiv - Microbiology 2022Quote: ... oligonucleotides were 5’-end-labelled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) with polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... in a 15 mg/mL solution and imaged in an IVIS Lumina II In Vivo Imaging System (Perkin Elmer) at 10-20 minutes post-injection ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted at 1 µg/mL in PBS and passively absorbed on multiwell plates (SpectraPlate-384 HB, Perkin Elmer) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed with 1M sorbitol and then resuspended in 1M sorbitol containing 30 µl glusulase per ml (# EE154001EA, Perkin Elmer) to a density of 5×108 cells/ml ...
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bioRxiv - Cell Biology 2023Quote: ... pulse–chase experiments were performed in labelling media supplemented with 2.5 μCi/ml [14C]proline (Perkin Elmer, Waltham, USA). After incubation in supplemented labelling media for 18 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... arrays were explanted from the mouse host and immediately incubated with cell culture media containing D-luciferin (0.15 mg/mL; PerkinElmer) for 10 minutes and then imaged using the In Vivo Imaging System (IVIS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined by incubating the cell with a luciferin solution (150 μg/mL; Perkin Elmer, MA, USA) and quantification of the bioluminescent signal emitted in an EnVision plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... and Hoechst (1µg/ml) for 1h at room temperature before washing with PBS and analysing on the Operetta CLS (PerkinElmer). Cells were imaged at 10X using the following channels ...
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bioRxiv - Biochemistry 2024Quote: ... The dry filter papers were placed in scintillation vials with 5 ml Ultima Gold F scintillation cocktail (Perkin Elmer) and scintillation was measured in a TriCarb 2900TR liquid scintillation analyzer (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... Plates were washed three times with PBS and bioluminescence measured (luciferin 0.3 mg/mL, Xenogen IVIS 50; Perkin Elmer). We used 24-well transwell inserts and plates (5 μm 29442-118 ...
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bioRxiv - Genomics 2023Quote: ... The metabolite extract was transferred into scintillation vials with 5 mL Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339).
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein pellet was then re-suspended in PBS and incubated in 4 mL of scintillation fluid (Insta-Gel Plus, PerkinElmer) for 24 h (to homogenize the samples ...
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bioRxiv - Molecular Biology 2019Quote: ... For micro-CT irradiation cells in 6 cm culture dishes or in 1.5 ml microcentrifuge tubes were scanned (Quantum FX, Perkin Elmer) in the low resolution mode (73 mm f.o.v. ...
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bioRxiv - Cancer Biology 2019Quote: ... Luminescence imaging carried out every 2-3 days using an IVIS luminescence imaging system 10 min after mice were injected with 10 μl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer). Mice were sacrificed by CO2 asphyxiation followed by cervical dislocation if they reached AAALAC-defined endpoints ...
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Biochemistry 2020Quote: ... was counted using a PerkinElmer Tri-Carb 2800RT liquid scintillation counter after dissolving the filter in 2 ml of scintillation liquid (Emulsifier Scintillator Plus, PerkinElmer).
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bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...
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bioRxiv - Biochemistry 2021Quote: ... The viral infectivity was measured by quantifying luciferase activity using 150ug/ml of XenoLight D-Luciferin bioluminescent substrate (PerkinElmer # 122799). Data was plotted using GraphPad Prism software.
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bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
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bioRxiv - Biophysics 2020Quote: ... separated free and bound radioligand and the radioactivity on each filter determined by the addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and count using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...
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bioRxiv - Microbiology 2022Quote: ... was phosphorylated in vitro with His-MBP-PknBMtb at 0.015 μg/μl for 30 minutes at room temperature with 0.15μCi/μl ATP [γ-32P] (5×10-8 μmol) (3000Ci/mmol, 10mCi/ml, PerkinElmer BLU002A250UC) and 2mM MnCl2 in buffer (50mM Tris ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Neuroscience 2021Quote: ... and seeded at 0.6 × 106 cells/ml on a poly-lysine/ laminin substrate in a 96 well plate (PerkinElmer #6005558). Primary cortical neurons were cultured for 5 days and then transfected using Lipofectamine 2000 (ThermoFisher # 11668019) ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were injected intra-peritoneally with XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate (15 mg/ml, 100ul/10g; Perkin Elmer). The mice were imaged with Xenogen IVUS Spectrum (Perkin Elmer) ...
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bioRxiv - Microbiology 2019Quote: ... and 500 µl were transferred to 2 ml Eppendorf tubes and spiked with 2.5 µCi of 32P-orthophosphoric acid (Perkin Elmer). The cultures were grown for two generations (OD600 0.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... from which aliquots of 10 μL were counted in vials with 3 mL OptiPhase HiSafe3 scintillation fluid (PerkinElmer, #1200-437). The fraction of charged tRNAAsn was calculated based on the charged tritium labeled aspartate incorporated into the pellet ...
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bioRxiv - Cancer Biology 2020Quote: ... The filters were washed with 5 ml wash buffer and counted using a liquid scintillation counter (Wallac 1450 MicroBeta Trilux, PerkinElmer). Exchange reactions with GST-Rab35 on glutathione-Sepharose beads were performed in the presence or absence of Arf5 ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM GTP and 0.25 mM CTP) and 0.5 µl of α-32P-CTP (3000 Ci/mmol, 10 mCi/ml Perkin Elmer) were supplemented into each reaction along with 1 ng of DNA template and 100 ng of competitor DNA ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown for imaging using standard conditions and imaged in low fluorescence medium containing 5 µg/ml Dextran Alexa Fluor 647 on an Opera Phenix (PerkinElmer) automated system as described above.
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bioRxiv - Genomics 2021Quote: ... and transferred to a plastic scintillation vial containing 10 mL of OptPhase HiSafe 3 scintillation cocktail (PerkinElmer, Waltman, MA, USA). All vials were counted for 10min using a Tri-Carb 2810 TR liquid scintillation analyzer (PerkinElmer ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Plant Biology 2023Quote: ... Radioactivity was measured from the extract and the trichloroacetic acid precipitated protein pellet for 5 min with a scintillation counter (Wallac 1414 WinSpectral v.1.40; Wallac Oy, Turku, Finland) using 1 ml of Ultima Gold™ (PerkinElmer) liquid scintillation cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... siScrambled or siAbdc1/2 Astrocytes were incubated overnight with DMEM without glucose supplemented with 1% FBS containing 1 μCi/mL 14C-Acetate ([1-14C] acetate, 58 mCi/mmol, PerkinElmer) and or α-cyclo or 50 μM C26:0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer, 10mCi/ml) using PCR products containing an upstream T7 promotor (see DNA constructs) ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells (9000 cells per 100 ml of culture medium) were seeded in each well of a 96-well plate CellCarrier-96 Ultra Microplates (6055302, PerkinElmer). Cells were cotransfected with 100 ng each of individual GFP-Rabs and mito-mScarlet-DENND6A or mito-mScarlet using jetPRIME ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Genetics 2023Quote: ... and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer). Cells were chased in RPMI supplemented with 0.4 mM methionine for 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...