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Citations for PerkinElmer :
1 - 50 of 97 citations for Kanamycin kinase type II NEO Klebsiella pneumoniae since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... aureus (SA) strain Xen36 containing a kanamycin resistant cassette (PerkinElmer American Type Culture Collection #49525) ...
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bioRxiv - Cell Biology 2021Quote: AlphaLISA® SureFire® Ultra™ cellular kinase immunoassays were used to measure endogenous kinase activity of p-mTOR (Ser2448) (PerkinElmer, #ALSU-PMTOR-C500), p-ERK 1/2 (Thr202/Tyr204 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a given histidine kinase was first autophosphorylated with [γ-32P]-ATP (Perkin Elmer) for 90 minutes at 30 °C to drive autophosphorylation and then mixed at a 1:4 molar ratio with a response regulator (1 μM EnvZ ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were radiolabelled with 32P-ATP by poly nucleotide kinase (PNK) (PerkinElmer PNK) for 1 hour at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Molecular Biology 2020Quote: mTOR kinase activity was monitored by LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET, PerkinElmer) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Plant Biology 2023Quote: ... or the CRI Maestro II (PerkinElmer, USA) live imaging system for GFP imaging in 35S:GFP Arabidopsis seedlings.
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Synthetic Biology 2022Quote: ... using the 384PP_Plus_GPSA fluid type into a ProxiPlate-384 Plus (Perkin Elmer 6008280) destination plate ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Developmental Biology 2024Quote: ... wild-type MEFs and KO in TET3 were seeded onto 96-well plate (PerkinElmer) and mitochondrial inhibitors were added at Krebs Ringer Phosphate Glucose buffer (KRPG ...
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bioRxiv - Cancer Biology 2023Quote: ... Mice were imaged using IVIS Lumina II (Perkin Elmer) following intraperitoneal injection of 150 μl of D-Luciferin substrate at 30 mg/ml (Sid Labs) ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaged on an IVIS Lumina II (Perkin Elmer)(23).
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bioRxiv - Biochemistry 2020Quote: ... 50 µg/mL PEG20,000 (SERVA; Cat. # 33138), ATP (Sigma; Cat. # A-7699) (variable, corresponding to ATP-Km of the respective kinase, [33P]- γ-ATP (PerkinElmer; Cat. # NEG302H): approx ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Dispersed HLOs were seeded in collagen type 1-coated CellCarrier-384 Ultra Microplates (PerkinElmer, 6057308) at a seeding density of 8,000 cells/well in hepatocyte growth media ...
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bioRxiv - Cancer Biology 2021Quote: ... and tumors imaged with the IVIS Lumina II device (PerkinElmer) 10 min afterwards ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... 9-1-1 having an N-terminal 7-residue kinase recognition sequence on Mec3 was radiolabeled to a specific activity of approximately 70 cpm/fmol with [γ-32P] ATP (PerkinElmer Life Sciences, Inc) using the recombinant catalytic subunit of cAMP-dependent protein kinase produced in E ...
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bioRxiv - Biophysics 2023Quote: ... a Gram-positive parental strain from the American Type Culture Collection (ATCC) were purchased from PerkinElmer Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... The electropherograms were analyzed using the LabChip GX II software (PerkinElmer).
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bioRxiv - Neuroscience 2020Quote: Quantification of cell types was performed using the Harmony High Content Imaging and Analysis Software (Perkin Elmer). Percentage of cells positive for desired marker versus total number of live cells was calculated ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR products were validated using a LabChip GX II (Perkin Elmer) and sequenced using PlateSeq PCR Kits (Eurofins ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were visualized using the Western Lightning ECL II Kit (Perkin Elmer) and exposed on X-Ray film.
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bioRxiv - Biochemistry 2020Quote: ... proteins were visualized using the Western Lightning ECL II Kit (Perkin Elmer) and exposed on X-Ray film (GE Healthcare Amersham Hyperfilm ECL ...
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bioRxiv - Microbiology 2023Quote: ... They were then placed in an IVIS Lumina II Spectrum system (PerkinElmer) and ventral and dorsal images acquired using Living Image v4.7.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... Prepared libraries were checked again using the RNA LabChip GX II (Perkin Elmer) and quantified using Qubit (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... and emission of light was measured with Victor II 1420 Multilabel Counter (PerkinElmer). Recorded bioluminescence was normalised by the optical density of cells in each well ...
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bioRxiv - Microbiology 2021Quote: ... bioluminescence imaging was performed using an IVIS Lumina II or Spectrum system (PerkinElmer), with acquisition time and binning adjusted according to signal intensity ...
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bioRxiv - Cancer Biology 2022Quote: ... was injected intraperitoneally prior to imaging with an IVIS Lumina II LT (PerkinElmer). Luminescence was quantified using LivingImage® software (PerkinElmer) ...
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bioRxiv - Genomics 2023Quote: In vivo bioluminescence imaging (BLI) was performed using the IVIS Lumina II (PerkinElmer). Mice treated with hydrodynamic injection were anesthetized using 2.5% isofluorane ...
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bioRxiv - Microbiology 2023Quote: ... hamsters were placed in a Lumina II In Vivo Imaging System (IVIS) (PerkinElmer) and images were acquired 10 – 20 minutes after d-luciferin administration using LivingImage v4.7 ...
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bioRxiv - Immunology 2022Quote: ... ii) 384-well plates (culture-treated, flat-optically clear bottom, Cell carrier plate, PerkinElmer) for morphological analyses on images acquired with the OPERA Phenix automated spinning disk confocal imager ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Cancer Biology 2023Quote: In ADM inhibition experiments wild type mouse organoids were imaged live after overnight acclimation using the Operetta High Throughput Screen imaging system (PerkinElmer) using 20× objective ...
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bioRxiv - Immunology 2020Quote: ... Staining of neutrophils (specifically myeloperoxidase activity) in LWT wild-type larvae was completed using TSA staining kit (Cy5-TSA Cyanine Kit, PerkinElmer, NEL705A001KT). Fish were incubated in a 1:100 ratio of Cy5-TSA:amplification diluent at 28°C for 10 minutes in the dark ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice and cell-associated activity was subsequently measured in a well-type gamma-counter (Wallac 2480 wizard, Perkin Elmer). In a separate binding assay equimolar concentrations (1.97 pM ...
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bioRxiv - Cell Biology 2019Quote: ... The pkd2-81KD spores were obtained by digesting the tetrads with Glusulase II (PerkinElmer, USA) and observed under a YE5s agar pad for 8-12 hrs with bright-field microscopy.