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Citations for PerkinElmer :
551 - 600 of 733 citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... which were exposed to X-ray film for 1 h and detected by a storage phosphor system (Cyclone, PerkinElmer, USA).
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bioRxiv - Microbiology 2020Quote: Overnight bacterial cultures were diluted 1:200 in LB broth and 50μl bacterial suspension was added per well in CellCarrier Ultra 96 plates (Perkin Elmer) and left in a static incubator at 37°C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
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bioRxiv - Microbiology 2022Quote: ... 293T-ACE2 target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer- compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
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bioRxiv - Biochemistry 2022Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2022Quote: ... Steady-state binding signal was obtained after incubation of reactions for 1 hr at 25° C using a EnSight Multimode plate reader (PerkinElmer) with a 30 ms excitation at 680 nm and emission measured at 615 nm after 140 ms ...
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bioRxiv - Immunology 2022Quote: ... and primary human GM-CSF monocyte derived macrophages (MDM) were cultured in collagen-1-coated CellCarrier Ultra 96-well black walled plates (PerkinElmer) at a ratio of 1425 HSC:28500 MDM in FBM (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Microbiology 2022Quote: The lipid compositions of the bacterial strains were determined after labeling with [1-14C] acetate (specific activity: 55 mCi/mmol; PerkinElmer) or [35S] sulfate (specific activity ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times in 1 x PBS and imaged using the Opera Phenix high-content microscope (Perkin Elmer) at 63x magnification.
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bioRxiv - Plant Biology 2023Quote: ... Radioactivity was measured from the extract and the trichloroacetic acid precipitated protein pellet for 5 min with a scintillation counter (Wallac 1414 WinSpectral v.1.40; Wallac Oy, Turku, Finland) using 1 ml of Ultima Gold™ (PerkinElmer) liquid scintillation cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were incubated in the dark for 1 hr at 37°C followed by measurement of fluorescence using an Envision plate reader (PerkinElmer) with standard AlphaScreenTM settings ...
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bioRxiv - Bioengineering 2022Quote: ... and Ethidium homodimer-1 (10μM) and cell death and viability data were measured using Operetta CLS High Content Analysis System (PerkinElmer, UK).
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Microbiology 2023Quote: ... Replication-competent HIV-1 stocks were normalized by p24Gag levels determined by ELISA (PerkinElmer Life Sciences, Inc., Boston, MA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 5x with TNT buffer and the second florescent signal was detected by incubating the samples in Cy5-TSA diluted 1:50 in Diluent buffer (PerkinElmer). The samples were washed at least 4x for 15 minutes each wash and then stained with Hoechst at 4°C overnight on a shaker before being put into Scale A2.
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence measurements (photons per second per cm2) were acquired over 1 minute and analysed using the Living Image software (PerkinElmer) using a constant size region of interest over the tissues ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM potassium acetate and 1 mM DTT followed by addition of 10 μM acetyl-CoA in a 1:10 ratio of 3H radiolabeled acetyl-CoA (PerkinElmer) to cold acetyl-CoA ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridization signals were detected by a chromogenic reaction using red chromogen dilution 1:3000 (PerkinElmer TSA Plus Cyanine 3 System). RNA-staining signal was identified as red punctate dots and clusters ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quantification was performed through spectrophotometric analysis at 260 nm using the Nanodrop ND-1000 Spectrophotometer and RNA quality was assessed using 1% agarose gel electrophoresis or the LabChip GX Touch (PerkinElmer). 5 µg of purified total RNA was combined with 5 µL of TURBO DNase buffer and 1 µL TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were used for orthotopic transplantation of 1×105 secondary tumor NES (tNES) cells and tumor growth was monitored using IVIS SpectrumCT In Vivo Imaging System (PerkinElmer) as previously described [14] ...
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bioRxiv - Physiology 2024Quote: ... or 120 nM of insulin in fresh serum-starvation medium for 30 min before adding 2 μL.mL-1 D-[14C(U)] glucose (NEC042B005MC; Perkin Elmer) for a further 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Cancer Biology 2024Quote: ... Eµ-Myc lymphoma cells grown in 6-well plates were spiked with 0.2 μCi/mL [1-14C]-acetate (PerkinElmer; NEC084H001MC) for the final 4 h of the experimental period ...
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bioRxiv - Cancer Biology 2024Quote: ... Image acquisition and quantification of POPO™-1 iodide staining intensity were done using the Harmony high-content imaging and analysis software 4.8 (PerkinElmer). Mesothelial clearance was performed by seeding MeT5A-GFP cells (15 ...
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bioRxiv - Biophysics 2021Quote: ... Depletion of NADPH is observed in 1 mL cuvettes with a path length of 1cm in a Lambda 650 UV/VIS spectrometer (Perkin Elmer) with attached water Peltier system set to 17°C ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed in Maleic buffer and then PBS at room temperature prior to incubation with 50 µl amplification diluent with 1 µl Tyramide-Cy3 (Perkin Elmer) for 3 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were pulsed with 1 μCi/well of [methyl-3H]TdR (70–90 Ci/mmol; NEN Radiochemicals, PerkinElmer, Akron, OH). After 16 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Neuroscience 2019Quote: ... before incubated with the Fluorophore Tyramide Amplification Reagent (1:200 in amplification diluent, TSA Plus multi-fluorophore detection kit, Fluorophore: Fluorescein, PerkinElmer, NEL753001KT). Sections were washed overnight in 1x PBS at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-mouse-HRP and anti-goat-HRP were used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Genomics 2020Quote: ... 63%, 40%, 25%) with 9:1 CSBL1:CBL1 buffer (Zeptosens, Bayer) using a Sciclone Caplier ALH3000 liquid handling root (Perkin Elmer). Diluted samples were spotted onto ZeptoChips (Zeptosens ...
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bioRxiv - Genetics 2021Quote: Cells were fixed in growth medium with 3.7% formaldehyde at room temperature for 30 minutes and their cell walls digested with a solution containing 50 μg/ml zymolyase and 1:50 glusulase (Perkin Elmer). The digested cells were mounted on poly-L lysine coated slides to immobilize them for microscopy ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM DTT, 300 mM KCl, 40 µM ATP, 40 µM GTP, 40 µM Biotin-11-CTP [Perkin Elmer] ...
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bioRxiv - Immunology 2021Quote: TZM-bl target cells (NIH AIDS reagent program) were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cells from each culture pulsed with 1 μCi [γ32P]-ATP having a specific activity of 30 Ci/mmol (Perkin Elmer) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Biochemistry 2020Quote: ... Fifty μl of each elution fraction was suspended in 1 ml of liquid scintillation reagent (Ultima Gold, PerkinElmer, Wellesley, MA, USA), and the radioactivity in each vial was measured using a scintillation counter ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...