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Citations for PerkinElmer :
151 - 200 of 252 citations for Integrin alpha 4 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Live imaging was done 4-8h later using a spinning disk confocal microscope (Perkin Elmer Ultra View ERS) equipped with a heating chamber (37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... filters were placed into scintillation vials with 4 mL Emulsifier-safe liquid scintillation fluid (Perkin Elmer, Waltham, MA) and analyzed using a liquid scintillation counter ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were fixed in 4% PFA and imaged with the Opera Phenix High Content Screening System (Perkin Elmer), acquiring at least 10 images per well ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were split again on days 4 or 5 as needed into a 96-well imaging plate (Perkin Elmer). A half media change was performed every other day if cells were not being split ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Cancer Biology 2022Quote: Mouse orthotopic xenograft models (4-5 mice per each group) were intravenously injected with 14C-acetate (3 μCi, PerkinElmer) in 200 μL saline and perfused with 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2022Quote: ... one ml of culture was labelled for 4 min at 30 °C using 30 μCi γ-[32P]ATP (PerkinElmer). Samples were incubated with 6% sucrose ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4 μL/well of cell culture supernatant was taken out and dispensed into OptiPlate-384 microplates (PerkinElmer, cat #: 6007290) that was pre-filled by 12 μL/well PBS by an EL406 ...
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bioRxiv - Immunology 2023Quote: ... uptake inhibition and degradation were determined in M[0] and M[IL-4] using 3H-choline chloride (Perkin Elmer) as previously described [4].
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bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were stained with HRP anti-Rabbit IgG and developed with Western Lightning Plus-ECL (Perkin Elmer) on X-ray film.
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated in horseradish peroxidase (HRP)- conjugated anti-rabbit IgG (1:1,000; Perkin Elmer, Boston, MA) for 1.5 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse IgG antibodies were obtained from PerkinElmer (Waltham, MA, USA). Vectashield antifade mounting medium with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biophysics 2021Quote: ... Cell fixation was performed 24h after transfection using 4% formaldehyde and cellular imaging was performed using an Operetta CLS (PerkinElmer). Three individual plate preparations were used as independent experiments for statistical significance (n=3) ...
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bioRxiv - Microbiology 2019Quote: ... which were incubated with various concentration of synthetic biotinylated TAR-RNA (biotin 5’-dT/GGU CUC UCU GGU UAG ACC AGA UCU GAG CCU GGG AGC UCU CUG GCU AAC UAG GGA ACC/3’-biotin dT) (Integrated DNA Technologies) at 4°C for 3 h in the AlphaLISA buffer (PerkinElmer). Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 4 h at 37°C and fluorescent signal intensity was quantified using the EnSpire Multimode Plate Reader (PerkinElmer) at excitation and emission wavelengths of 540 and 612 nm respectively.
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bioRxiv - Molecular Biology 2020Quote: ... The protein pellet was then re-suspended in PBS and incubated in 4 mL of scintillation fluid (Insta-Gel Plus, PerkinElmer) for 24 h (to homogenize the samples ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
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bioRxiv - Neuroscience 2020Quote: ... In situ hybridization using nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate and double color in situ hybridization using TSA Plus (PerkinElmer) were performed as previously described (Yamagata et al.,1999 ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Biophysics 2020Quote: ... separated free and bound radioligand and the radioactivity on each filter determined by the addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and count using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Neuroscience 2022Quote: ... To quantify α1-3;5GABAAR79 sections were incubated for 60 minutes at 4°C in 1nM [3H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [3H]flumazenil with 10 µM flunitrazepam (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were monitored every 3-4 days by non-invasive luciferase imaging by intraperitoneal injection of 150 mg/kg Luciferin (PerkinElmer) and quantification of luciferase activity using Imaging Systems (IVIS Spectrum System ...
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bioRxiv - Biochemistry 2021Quote: Structural models of polyenoyl tetramic acids 3 and 4 were built and energy minimized using Chem3D v.16.0 (Perkin Elmer). The terminal region of polyenes ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µL of RNA was 3’-end-labeled with 32pCp (prepared by incubating 16.5 µl of γ-32P-ATP (PerkinElmer), 1 µl of T4 polynucleotide Kinase ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aerrane (Isoflurane, Baxter) inhalation was performed for anesthesia using the RAS-4 Rodent Anesthesia System (Perkin Elmer, Waltham, MA, USA) for experiments with IVIS Spectrum CT living imaging system (Perkin Elmer ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated for 4 h at 37°C and then the supernatant was removed and incubated with scintillation fluid (PerkinElmer) overnight at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase were used at 1:10,00 dilution (PerkinElmer). Immunoblots were developed using PerkinElmer Western Lightning Plus-ECL and imaged with a GE Healthcare Amersham Imager 600 ...
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bioRxiv - Molecular Biology 2020Quote: ... In brief, using a rabbit antimelatonin antiserum (PF1288; P.A.R.I.S., Paris, France) and 2-Iodomelatonin (NEX236050UC; PerkinElmer, Boston, Massachusetts) as tracer RIA was performed ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in horseradish peroxidase-conjugated anti-rabbit IgG (1:1000; cat# NEF812001EA, Perkin Elmer, Boston, MA) for 1.5 hours ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Plant Biology 2022Quote: ... 5-mm root segments away from the apical 2 mm root tip and 2 mm away from the root-shoot junction were carefully cut and soaked overnight in 4 ml of liquid scintillation fluid (Ultima Gold, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Physiology 2020Quote: ... Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by: addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and the retained radioactivity determined using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mm root segments away from the apical 2 mm were carefully cut and soaked for overnight in 4 mL liquid scintillation fluid (ULTIMA GOLD, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...