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Citations for PerkinElmer :
101 - 150 of 1494 citations for Human Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) or by qPCR ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 pseudovirus productions were quantified by p24 ELISA (Perkin Elmer Life Sciences).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and protein expression signals were detected using a chemiluminescence detection kit (Perkin Elmer, Waltham, MA, USA) and visualised using a chemiluminescence imaging system (Fusion Solo S ...
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bioRxiv - Neuroscience 2022Quote: ... Binding was performed with the selective MOR agonist [3H]DAMGO (53.7 Ci/mmole, Perkin Elmer, Boston, MA) in 50 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Neuroscience 2023Quote: ... the binding sites of the secondary antibodies were detected using Western Lightning ECL Pro (PerkinElmer, MA, USA). Chemiluminescence was visualized using the Fusion Solo S imaging system (Vilber Bio Imaging ...
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bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
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bioRxiv - Neuroscience 2021Quote: ... monomer equivalents of fibrils (α-synuclein or ASC) were coated onto high-binding 384-well SpectraPlates (Perkin Elmer) as serial dilutions (13-fold 1:2 serial dilution) ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... scintillation cocktail and binding quantified using scintillation counting for H3 on an Tri-Carb 2810TR scintillation counter (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... ELISA absorbance was read at 450nm for 0.1 second on a Victor X5 plate reader (PerkinElmer).
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bioRxiv - Cell Biology 2019Quote: ... ELISA plates were read at 450 nm using a Wallac 1420 Victor2 Microplate Reader (Perkin Elmer, provided by Don Jarvis at the University of Wyoming) ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Developmental Biology 2020Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... and AKT 1/2/3 (p-S473) Assay Kits (PerkinElmer). Cell lysis and SureFire Assay were performed following the manufacturer’s instructions.
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... 100 nM GBR12909 (to block binding to dopamine transporter) and 50 pM [125I]RTI-55 (NEX272, Perkin Elmer, Boston, MA) were added to the buffer ...
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bioRxiv - Biophysics 2023Quote: ... Leucine affinities for WT LeuT and mutants displaying WT substrate affinities (A22V, A22S) were determined by saturation binding of [3H]leucine (25 Ci/mmol) (PerkinElmer), with unspecific binding corrected for by the addition of 100 µM unlabelled leucine ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Biochemistry 2019Quote: ... The BMAL TAD probe was diluted to a working concentration of 50 nM in assay buffer and binding was monitored by changes in fluorescence polarization with an EnVision 2103 multilabel plate reader (Perkin Elmer) with excitation at 531 nm and emission at 595 nm ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit; PerkinElmer). For Flag immunoprecipitations ...
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bioRxiv - Neuroscience 2019Quote: ... The optical density was measured by an ELISA reader (EnSpire, Multimode Plate Reader, PerkinElmer do Brasil, SP, Brasil).
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bioRxiv - Molecular Biology 2023Quote: ... Cellular viability was determined using the ATPlite 1-step kits (PerkinElmer). 50 µl of ATPlite solution was added in 96-well plates for 15 min ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Daily calibration and normalization of the PET scanner were performed using a 68Ge/68Ga phantom and cross-calibration factor was determined with a γ-counter (Wizard2; PerkinElmer). Synthesis and formulation of the radiotracer were done as described in (Holschbach et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Microtiter 384-well Proxiplates for the ThermoFRET stability assay and 384-well Optiplates for association binding experiments were obtained from PerkinElmer (Beaconsfield, UK).
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bioRxiv - Neuroscience 2024Quote: ... and 50 µl of radioligand diluted in binding buffer ([3H]-N-methylspiperone: 0.4 nM final concentration, Perkin Elmer, Waltham, MA; [3H]-(R)-(+)-7-OH-DPAT ...
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bioRxiv - Molecular Biology 2019Quote: ... PerkinElmer chemagic FFPE DNA kit (chemagic MSM 1; Perkin Elmer, Baesweiler, Germany), and Applied Biosystem’s MagMAX™ FFPE DNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... The saturation binding assay was conducted using an 8-point dose-dependent concentration of [3H] ketanserin (maximal concentration 20 nM) (Perkin Elmer, Waltham, MA) with brain crude membranes (25 μg membrane proteins ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... with HRP goat anti-mouse IgG Ab (31430, 1:20,000) for 1 h and developed using a Western Lightening Enhanced Chemiluminescence kit (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated in 1:200 TSA/amplification buffer (TSA Plus Fluorescence Kits; PerkinElmer) for 30 min to develop signals ...
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bioRxiv - Developmental Biology 2019Quote: ... all samples were incubated in TSA Plus Cyanine5 Evaluation kit (1:50; PerkinElmer) for signal amplification at room temperature for 1 hr with protection from light ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm-pore size filter and quantified for viral Cap24 content using enzyme-linked immunosorbent assay (ELISA) (Perkin Elmer, # NEK050A) as previously published (41 ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Saturation binding assay was performed to quantify the receptor expression and equilibrium dissociation constant of the radioligand [3H]-NMS (PerkinElmer, specific activity 80 Ci/mmol). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...