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Citations for PerkinElmer :
501 - 550 of 726 citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: We used hairless SKH-1 mice for live animal imaging on IVIS Spectrum In vivo Imaging System (Perkin Elmer). For ovalbumin quantification ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed 3 times with wash buffer (PBS + 1 mM CaCl2 + 0.1% BSA) before adding Microscint-20 (PerkinElmer) for tritium concentration measurement using a TriLux MicroBeta2 plate reader (PerkinElmer) ...
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bioRxiv - Plant Biology 2023Quote: ... samples were incubated for 1h in 1:50 of a digoxigenin-tyramide signal amplification solution (TSA Plus DIG, PerkinElmer) prepared according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: The lipid composition of bacterial strains was determined after labeling with [1-14C] acetate (60 mCi/mmol; Perkin Elmer), [32P] phosphate (1 Ci/mmol ...
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bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 nmol of chemerin-9 in 25 μL iodination buffer (0.5 M sodium phosphate, pH 7.4) were mixed with 1 mCi carrier-free Na125I (NEZ033L010MC, PerkinElmer, Waltham, USA) in an HPLC glass vial with microvolume insert ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Bioengineering 2020Quote: ... Azide functionalization was confirmed with Fourier-transform infrared spectroscopy (FTIR, Supplemental Figure 1, F Perkin Elmer Frontier MIR/NIR spectrometer) and quantified with electrospray ionization-mass spectrometry (ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1×104 HEK-293T cells were seeded in culture medium without antibiotics in white 96-well culture plates (Perkin Elmer) prior to transfection ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
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bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Neuroscience 2020Quote: Larval stage 1 animals (L1) were imaged in a UltraView VoX spinning-disc confocal microscope (PerkinElmer Life and Analytical Sciences). Embryonic imaging was performed via dual-view inverted light sheet microscopy (diSPIM)64,65 ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Biochemistry 2020Quote: ... was transferred to 384-well plates and diluted to 1 mM in DMSO using a JANUS Varispan Automated Workstation (PerkinElmer). The U2AF1S34F–U2AF2–SF1 protein and FlDEK variant oligonucleotide were plated at a total 20 μL reaction volume per well ...
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bioRxiv - Cell Biology 2019Quote: A centrifuge-free RABiT-II system was used for automated sample processing after culturing: The system consists of two part: the first (Figure 1) has an automated liquid-handling system (JANUS, PerkinElmer) with integrated automated microplate handler (Twister3 ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Plant Biology 2020Quote: ... or without (for pPcUBI (I) and (II)) 1 μM Brassinazole medium were imaged using an Ultra View Vox Spinning disc confocal imaging system (PerkinElmer) mounted on an Eclipse Ti inverted microscope (Nikon ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Microbiology 2021Quote: ... an equivalent of 500 ng of p24 Gag was lysed in buffer with 1% Triton (ELISA kit, Alliance Perkin Elmer) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... Three-plex detection of IL-4Ra on glutaminergic and GABAergic neurons was achieved using Opal 520 (1:1000, Perkin Elmer), Opal 570 (1:1500 ...
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... which were exposed to X-ray film for 1 h and detected by a storage phosphor system (Cyclone, PerkinElmer, USA).
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bioRxiv - Microbiology 2020Quote: Overnight bacterial cultures were diluted 1:200 in LB broth and 50μl bacterial suspension was added per well in CellCarrier Ultra 96 plates (Perkin Elmer) and left in a static incubator at 37°C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
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bioRxiv - Microbiology 2022Quote: ... 293T-ACE2 target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer- compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
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bioRxiv - Biochemistry 2022Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).