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Citations for PerkinElmer :
1 - 50 of 99 citations for Histone H3 mono methyl K36 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... and AlphaLISA unmodified Histone H3 Lysine 4 (H3K4) Cellular Detection Kit (AL719, PerkinElmer) for normalization ...
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bioRxiv - Genetics 2020Quote: ... Replicates were tested with both AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit (AL720, PerkinElmer) and AlphaLISA unmodified Histone H3 Lysine 4 (H3K4 ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM) (PerkinElmer)] ...
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bioRxiv - Immunology 2022Quote: ... [Methyl-14C] Methylated (PerkinElmer Life Sciences), TRIzol (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... [Methyl-3H]-Choline Chloride (Perkin Elmer) (0.3 mCi/kg ...
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bioRxiv - Pathology 2022Quote: ... to [methyl-3H]-SAM (PerkinElmer, Cat#NET155001MC). Reactions were incubated for 1 hr at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 5 µM [methyl-3H] S-adenosyl methionine (PerkinElmer), 10 µM substrate RNA/DNA probe ...
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bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
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bioRxiv - Biochemistry 2022Quote: ... S-[methyl-3H] (3H-SAM) (PerkinElmer, 18.0 Ci/mmol); and 0.1 μM purified DNMT3A2 in assay buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 0.76 µM labelled [methyl-3H] -AdoMet (Perkin Elmer) for 3 hours at 25°C ...
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bioRxiv - Genomics 2023Quote: ... only 100nM were radioactive ([Methyl-3H]-Choline Chloride; Perkin Elmer #NET109001MC), the rest was unlabeled (Choline Chloride ...
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bioRxiv - Immunology 2019Quote: ... Mono-color staining of each of the antibodies was performed using the OPAL 7 color kit (PerkinElmer), using the same protocol as IHC ...
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bioRxiv - Immunology 2021Quote: ... 1 µCi of S-adenosyl [methyl-3H] methionine (0.5 μCi/μl; PerkinElmer) in reaction buffer (500 mM Tris–HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μM S-adenosyl-L-[methyl-3H] methionine (specific activity 18Ci/mmol, PerkinElmer). For unmodified DNA duplex ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.55μM S-adenosyl-L-[methyl-3H] methionine (specific activity 18Ci/mmol, PerkinElmer), 1.9μM AdoMet in 50mM Tris–HCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Histone methyltransferase assays were performed in 96-well half-area optiplates (PerkinElmer) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Live imaging of all alveolar organoid mono- and co-cultures was performed using the Operetta CLS high-content analysis system (Operetta CLS, PerkinElmer) at time points 5 ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μM mononucleosomes and 5.0 μM S-[methyl-14C]-adenosyl-l-methionine (PerkinElmer, #NEC363050UC) in the presence or absence of stimulatory or control peptides in concentrations as indicated in the text ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.9µM (0.5 µCi) S-[Methyl-3H]-Adenosyl-L-methionine (17.2 mCi/mL, Perkin Elmer)) and incubated at 30 °C ...
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bioRxiv - Immunology 2021Quote: ... after incubation for 16 h with 1 µCi/ml [methyl-3H]-thymidine (Perkin Elmer). Data were expressed as counts per min (Cpm ...
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bioRxiv - Genetics 2020Quote: ... 2.5 μM S-adenosyl-L-[methyl-3H]methionine (specific activity 18 Ci/mmol, PerkinElmer) in 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... 0.093% of the total choline was radioactive ([Methyl-3H]-Choline Chloride; Perkin Elmer #NET109001MC)(Choline Chloride ...
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bioRxiv - Biochemistry 2023Quote: ... 0.55 μM S-adenosyl-L-[methyl-3H] methionine (specific activity 58.9 Ci/mmol, PerkinElmer) in 50 mM Tris–HCl (pH 7.2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM DTT and 2 μCi/mL S-adenosyl-L-[methyl-14C] methionine (PerkinElmer). After 3h incubation ...
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bioRxiv - Biochemistry 2020Quote: ... 0.56 µM S-adenosyl-L-[methyl-3H]methionine with a specific activity of 18 Ci/mmol (PerkinElmer), 1.96 µM nonradioactive SAM ...
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bioRxiv - Genetics 2020Quote: ... DNA methylation was measured by the incorporation of tritiated methyl groups from radioactively labeled SAM (Perkin Elmer) into the biotinylated substrate using an avidin–biotin methylation plate assay47 ...
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bioRxiv - Molecular Biology 2020Quote: ... scintillation cocktail and binding quantified using scintillation counting for H3 on an Tri-Carb 2810TR scintillation counter (Perkin Elmer).
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bioRxiv - Genetics 2019Quote: ... PRMT7 wild-type and mutants were incubated with putative substrates in PBS with 2 μCi of S-adenosyl-[methyl-3H]methionine ([3H]AdoMet) (55-85 Ci/mmol, PerkinElmer) for 18 h at 26°C ...
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bioRxiv - Genomics 2023Quote: ... at a total concentration equivalent to 0.8 μM nucleosomes and 5.0 μM 14C-SAM (S- [methyl-14C]-adenosyl-l-methionine (PerkinElmer, no. NEC363050UC)) ...
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bioRxiv - Microbiology 2024Quote: ... Of each sample three replicates and two formaldehyde-fixed (final conc. 1.85 %) adsorption blanks were spiked with [methyl-3H]-thymidine and L-[14C(U)]-leucine (Perkin Elmer) at saturating concentrations of 20 nM and 150 nM ...
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bioRxiv - Microbiology 2019Quote: ... 25µL of the warmed suspension was mixed with 150µL of MOPS-CDM containing 150µCi mL-1 of H3[32P]O4 (2mCi mL-1 in Water; PerkinElmer, Waltham, MA) for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were pulsed with 1 μCi/well of [methyl-3H]TdR (70–90 Ci/mmol; NEN Radiochemicals, PerkinElmer, Akron, OH). After 16 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the SPOT arrays were incubated in methylation buffer containing additionally 50 nM SETD6 and 0.76 µM labelled [methyl-3H]-AdoMet (Perkin Elmer Inc.) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: DNA methylation of replicating sperm or erythrocyte nuclei in egg extract was assayed by the incorporation of 3H-SAM (S-[methyl-3H]-adenosyl-L-methionine; Perkin Elmer, NET155H). Demembranated sperm nuclei were prepared as published previously (86) ...
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bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
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bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
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bioRxiv - Molecular Biology 2021Quote: ... with or without EcoRI hairpin oligo or NAD+ in histone methyltransferase buffer and 6 µM of radiolabeled [3H] AdoMet (Perkin Elmer Life Science # NET155V001MC). Recombinant human histone H4 (New England Biolabs # M2504S ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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bioRxiv - Microbiology 2023Quote: ... or α-rabbit (PerkinElmer) (for CdnL) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... and then with anti-rabbit [125I]-IgG (PerkinElmer) for 2hr at 4° ...