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Citations for PerkinElmer :
151 - 197 of 197 citations for Goat Anti Mouse IgG Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and then incubated with anti-FLAG antibody conjugated to APC (αFLAG-APC; PerkinElmer) diluted 1:100 in PBS + 0.1% BSA at a ratio of 30 μL labeled antibody:107 cells ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-DIG-POD signal was amplified with TSA Biotin System Kit (Perkin Elmer) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... The anti-fluorescein antibody was visualized by using the TSA Plus Fluorescein System (PerkinElmer) in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and lastly 20 μg mL−1 Anti-c-Myc AlphaLISA® Acceptor bead (PerkinElmer) completed the total reaction volume of 25 μL ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with an anti-fluorescein horseradish peroxidase conjugate (1:500, PerkinElmer, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary anti-HRP conjugate antibodies were used and immunoreactivity was visualized using Western LightingTM (PerkinElmer). Immunoreactivity was developed on film or using a ChemiDoc™ imaging system (Biorad) ...
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bioRxiv - Developmental Biology 2022Quote: ... Then those sections were stained with anti-MYC antibody with TSA plus fluorescein system (Perkin Elmer) followed by antigen retrieval to remove antibodies ...
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bioRxiv - Physiology 2024Quote: ... Fluorescein was detected with a horseradish peroxidase-conjugated anti-fluorescein antibody (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized by using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... Signals from anti-OAZ and Lmx1a antibodies were amplified using the Tyramide Signal Amplification Kit (Perkin Elmer), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Physiology 2024Quote: ... fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Samples were resuspended in Optimem with a 1:1000 dilution of APC conjugated to anti-Flag (PerkinElmer) and 1:500 dilution Alexa Flour 488 conjugated to anti-HA (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... the GlyT1 probe was detected using anti-DIG-HRP (1:750) and tyramide amplification (Perkin Elmer TSA Cy3). This was followed by the immunohistochemistry protocol to amplify the GFP signal in Dact2-GFP retinas ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense RNA probes labeled with DNP were used in all in situ hybridizations and probe detection was performed using a HRP-coupled anti-DNP antibody (PerkinElmer) and a fluorescein coupled tyramide (Vize et al. ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and the amplification steps were repeated using anti-DNP-HRP Conjugate (PerkinElmer) and TSA Plus Cyanine 3 (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Genomics 2023Quote: ... blood was collected in EDTA anti-coagulant BD Vacutainer tubes and genomic DNA was extracted using the Chemagic MSM I Instrument (PerkinElmer) following the manufacturer’s recommendations and stored at −20°C before PCR 44 ...
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bioRxiv - Cell Biology 2023Quote: ... BMK-dko cells stably expressing a mCerulean3-fused anti-apoptotic protein were seeded at 4000 cells/well into a CellCarrier-384 Ultra Microplate (PerkinElmer). 24 hours later ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with different concentrations of GST-hnRNPA2B1 (0-60 nM) in the presence of streptavidin Donor beads and anti-GST-Acceptor beads (PerkinElmer) (20 µg/ml final concentration) ...
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bioRxiv - Immunology 2022Quote: ... bound cytokines were detected by biotinylated anti-IFN-γ (clone 4s.B3) and quantified using a Victor2 D time-resolved fluorometer (Perkin Elmer). The % activity was calculated by dividing the SI of mutated peptides by the SI of the wild-type peptide ...
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bioRxiv - Immunology 2021Quote: ... Cells were immunostained with DAPI and Alexa488-conjugated anti-N antibody (CR3009; produced in-house) and imaged using an Opera Phenix (Perkin Elmer). The ratio of infected (Alexa488+ ...
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bioRxiv - Biophysics 2022Quote: ... The anti-puromycin fluorescence in the cytoplasm was detected automatically using the Opera Phenix® Plus High Content Screening System (PerkinElmer). The cytoplasm was detected automatically using the HARMONY v4.8 software.
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with an anti-ERα antibody (F-10) overnight followed by Cy3 Tyramide signal amplification (PerkinElmer-TSA-Plus-Cy3) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated overnight at 4ºC with horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:1000 and 1:500 for detection in PMm/PMg and other nuclei, respectively; PerkinElmer, Waltham, MA) and Dylight 549-conjugated goat anti-rabbit IgG (diluted 1:500 ...
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bioRxiv - Microbiology 2020Quote: ... FISH detection was performed using anti-biotin antibody (SA-HRP) and a FITC/Cy5 coupled secondary antibody with the TSA Plus system (Perkin Elmer, USA). Coverslips were stained with Hoechst ...
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bioRxiv - Neuroscience 2019Quote: ... The secondary and Opal™ steps were then repeated using the anti-rabbit secondary and Opal™ 650 reagent (1:1000; PerkinElmer). Sections were then washed 3x for 2 min each in TBS-T and counterstained with DAPI to label nuclei.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:500 with DIG-1, PerkinElmer, Foster City, CA) for 1 h and washed twice with DIG-1 for 5 min each time ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
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bioRxiv - Neuroscience 2020Quote: ... Reaction termination and detection were performed using 1X LANCE detection buffer with 20 mM EDTA and 2 nM LANCE Ultra Europium anti-phospho Glycogen Synthase (Ser641) (Perkin Elmer #TRF0220-M).
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...